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  • 1
    Electronic Resource
    Electronic Resource
    Reciprocal biological activities of the cyclic tetrapeptides chlamydocin and HC-toxin (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 348-350 
    Details
    ISSN: 1420-9071
    Keywords: Chlamydocin ; HC-toxin ; phytotoxin ; cytostatic agent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Chlamydocin, a potent cytostatic agent against cultured mammalian cells, and HC-toxin, a host-specific phytotoxin, are cyclic tetrapeptides containing the same epoxide α-amino acid. We show here that these compounds have reciprocal biological activity; HC-toxin is cytostatic against cultured mastocytoma cells, and chlamydocin has host-specific toxin activity against maize. Chlamydocin and another related cyclic peptide, Cyl-2, are less host-specific than HC-toxin because maize tolerant to HC-toxin is more sensitive to chlamydocin and Cyl-2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02004498
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  • 2
    Electronic Resource
    Electronic Resource
    Organelle assortment and mitochondrial DNA rearrangements in Brassica somatic hybrids and cybrids (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 85 (1992), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Protoplast fusion permits manipulations of organelle genomes not readily achieved by other in vitro procedures or sexual crosses. Although considerable information is now available about the fate of chloroplasts and mitochondria in fusion products of various genera, many additional questions about factors affecting organelles after fusion remain to be answered. Brassica species are particularly favorable materials for such studies. Organelle assortment, mitochondrial DNA (mtDNA) recombination, and plant phenotypes observed after fusion of protoplasts from cytoplasmic male sterile B. oleracea with protoplasts from B. campestris, B. oleracea or B. napus are described. The somatic hybrids and cybrids obtained at Cornell have been used for detailed studies of recombinant mtDNA, including correlation of a specific mtDNA region with the ogura type of cytoplasmic male sterility, and have provided plant materials for possible use in hybrid breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1992.tb04742.x
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  • 3
    Electronic Resource
    Electronic Resource
    Resynthesis of rapeseed (Brassica napus L.): A comparison of sexual versus somatic hybridization (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 115 (1996), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sexual and somatic Brassica napus hybrids produced from the same parental plants were compared. Sexual crosses between a white-flowered, self-compatible broccoli selection (B. oleracea var. italica, cc genome) as the maternal parent and a flowering pak choi accession (B. chinensis, aa genome) yielded one unique spontaneous hybrid and four hybrids through embryo rescue. Thirty-nine somatic hybrids were recovered from a protoplast fusion experiment. Hybridity was confirmed by morphology, isozyme expression, flow cytometry, and DNA hybridization. Sexual and somatic hybrids exhibited differences in leaf morphology, flower colour, flowering habit, and organellar inheritance. Sexual hybrids were all fertile amphidiploids (2n = 38, aacc) following spontaneous chromosome doubling. All somatic hybrids had high nuclear DNA contents; most were probably hexaploids (aaaacc or aacccc) from the fusion of three portoplasts. Two initially sterile hexaploid (aaaacc) regenerates eventually set selfed seed after the loss of the putative extra aa genome following regrowth from axillary buds. A bias toward inheritance of B. chinensis chloroplasts was observed with somatic hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1996.tb00940.x
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  • 4
    Electronic Resource
    Electronic Resource
    High resolution physical mapping of 45S (5.8S, 18S and 25S) rDNA gene loci in the tomato genome using a combination of karyotyping and FISH of pachytene chromosomes (1996)
    Springer
    Chromosoma 104 (1996), S. 545-550 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Karyotyping in combination with fluorescence in situ hybridization (FISH) on tomato pachytene chromosomes allowed identification and mapping of a major 45S (5.8S, 18S and 25S) rDNA site on the satellite of 2S and four minor loci, each at a proximal knob on 2L, 6S, 9S and 11S. Thus, the 45S rDNA loci are all located in heterochromatic regions. The five 45S sites are all transcriptionally active as evidenced by a maximum of ten nucleoli in meiotic cells at telophase or interphase. The 45S rDNA loci, as well as the 5S rDNA locus on 1S, were highlighted by chromomycin A3, a GC-specific DNA ligand; this result is consistent with the high GC content of the rDNA genes. Satellite size varied dramatically between genotypes. Enzymatic maceration of tomato anthers followed by squashing in acetocarmine produced high quality chromosomal preparations and subsequent FISH images by reducing the strong autofluorescence inherent in the nucleolus and cytoplasm of tomato meiotic cells. Our protocol has potential in the construction of an integrated cytological, classical and molecular map of tomato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352294
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  • 5
    Electronic Resource
    Electronic Resource
    High resolution physical mapping of 45S (5.8S, 18S and 25S) rDNA gene loci in the tomato genome using a combination of karyotyping and FISH of pachytene chromosomes (1996)
    Springer
    Chromosoma 104 (1996), S. 545-550 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Karyotyping in combination with fluorescence in situ hybridization (FISH) on tomato pachytene chromosomes allowed identification and mapping of a major 45S (5.8S, 18S and 25S) rDNA site on the satellite of 2S and four minor loci, each at a proximal knob on 2L, 6S, 9S and 11S. Thus, the 45S rDNA loci are all located in heterochromatic regions. The five 45S sites are all transcriptionally active as evidenced by a maximum of ten nucleoli in meiotic cells at telophase or interphase. The 45S rDNA loci, as well as the 5S rDNA locus on 1S, were highlighted by chromomycin A3, a GC-specific DNA ligand; this result is consistent with the high GC content of the rDNA genes. Satellite size varied dramatically between genotypes. Enzymatic maceration of tomato anthers followed by squashing in acetocarmine produced high quality chromosomal preparations and subsequent FISH images by reducing the strong autofluorescence inherent in the nucleolus and cytoplasm of tomato meiotic cells. Our protocol has potential in the construction of an integrated cytological, classical and molecular map of tomato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352294
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  • 6
    Electronic Resource
    Electronic Resource
    Novel mitochondrial genomes in Brassica napus somatic hybrids (1992)
    Springer
    Current genetics 22 (1992), S. 243-249 
    Details
    ISSN: 1432-0983
    Keywords: Brassica ; Somatic hybrids ; Cytoplasmic male sterility ; Mitochondrial DNA rearrangement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial genomes of nine male-fertile and two Ogura cytoplasmic male-sterile (cms) Brassica napus somatic hybrids were probed with 46 mitochondrial DNA fragments. The distribution of information obtained from each fusion partner was not random. Several regions, including the coxI gene and a major recombination repeat sequence, were always derived from the Brassica campestris fusion partner, and some regions were always derived from the Ogura mitochondrial genome. Novel fragments occurred in seven distinct regions. Some of the rearrangement breakpoints were located near the evolutionary breakpoints relating the mitochondrial genomes of the Brassica species. The sizes of the mitochondrial genomes in the somatic hybrids ranged from 224.8 to 285.3 kb. A direct correlation between a specific gene and the cms phenotype was not observed; however, a possible cms-associated region was identified. It corresponds to a region that was identified through analysis of fertile revertants from a cms B. napus cybrid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351732
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  • 7
    Electronic Resource
    Electronic Resource
    Plant regeneration from leaf protoplasts of Brassica oleracea var. italica CV Green Comet broccoli (1986)
    Springer
    Plant cell reports 5 (1986), S. 61-64 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure is described for regeneration of plants from leaf protoplasts of the hybrid broccoli cultivar, Green Comet (Brassica oleracea var italica). The totipotency of protoplasts isolated from plants regenerated from hypocotyl explants (GCR) was greater than that of protoplasts from plants grown directly from seed (GC). Using medium B developed by Pelletier et al (1983), division efficiencies greater than 70% were obtained in leaf protoplasts isolated from GCR. Approximately 1% of these protoplasts formed calli on solidified medium; 77% of the calli regenerated shoots. In contrast, protoplasts from seed-grown material showed a lower division efficiency (15–22%) and fewer protoplast-derived calli produced shoots. Some of the 178 protoplast-derived plants grown to maturity had variant phenotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269720
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  • 8
    Electronic Resource
    Electronic Resource
    Regeneration of plants from protoplasts of Capsella bursa-pastoris and somatic hybridization with rapid cycling Brassica oleracea (1999)
    Springer
    Plant cell reports 18 (1999), S. 412-417 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsCapsella bursa-pastoris ; Rapid-cycling Brassica oleracea ; Protoplasts ; Intertribal somatic hybrids ; Alternaria brassicicola
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fertile rooted plantlets were recovered from leaf mesophyll protoplasts of Capsella bursa-pastoris. Protoplasts cultured over a feeder layer of Brassica napus cells produced 221 colonies, 7 of which regenerated multiple plantlets. The nuclear DNA content of most regenerates varied from 0.89 to 1.0 pg/nucleus, close to the value for seed-grown C. bursa-pastoris (0.94±0.03 pg/nucleus). Two regenerants had a tetraploid DNA content (1.8– 2.0 pg). Plants with a DNA content close to Capsella produced seeds, both in vitro and in soil. Intertribal somatic hybrids were obtained by polyethylene glycol-mediated fusion of untreated C. bursa-pastoris protoplasts with iodoacetate-treated protoplasts of rapid-cycling B. oleracea. Plants were confirmed as somatic hybrids by isozyme and RAPD analysis. The nuclear DNA content of the hybrids ranged from 3.2 to 6.4 pg, higher than the sum of the parental genomes. One of two hybrids tested was resistant to Alternaria brassicicola, like the Capsella fusion partner. Hybrids rooted easily and produced sterile flowers when transplanted to soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050595
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  • 9
    Electronic Resource
    Electronic Resource
    Inheritance of callus formation ability in anther cultures of rice, Oryza sativa L. (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 113-116 
    Details
    ISSN: 1432-2242
    Keywords: Microspore ; Anther culture ; Callus formation ; Diallel analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inheritance of ability to form callus in rice anther culture was studied using the diallel technique. Anthers containing uninucleate microspores from two japonica cultivais (‘Minehikari’ and ‘Taipei 309’), two indica cultivars (‘Mingolo’ and ‘Suweon 290’), and 12 F1's of the diallel crosses involving these four parents were cultured on Chaleffs R2 medium and evaluated for callus induction. The parents showed significant differences in anther callus formation, from 41.9% (‘Taipei 309’) to 0% (‘Suweon 290’). Callus induction ability was inherited as a recessive character conditioned by a single block of genes. Additive gene effects were predominant. The japonica types seemed to be good combiners for callus induction. The order of dominance among the four parents was ‘Suweon 290’, ‘Mingolo’, ‘Minehikari’ and ‘Taipei 309’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275308
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  • 10
    Electronic Resource
    Electronic Resource
    Chromosome doubling and mode of reproduction of induced tetraploids of eastern gamagrass (Tripsacum dactyloides L.) (1998)
    Springer
    Plant cell reports 17 (1998), S. 881-885 
    Details
    ISSN: 1432-203X
    Keywords: Key words Eastern gamagrass ; Tripsacum ; Chromosome doubling ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eastern gamagrass, (Tripsacum dactyloides L.) is a perennial, warm-season grass that is being developed as a forage plant. Shoots were derived from callus initiated from immature embryos and immature inflorescences of diploid (2n=2x=36) gynomonoecious eastern gamagrass. These shoots were induced to microtiller in the presence of 3 mg/l benzyladenine. Amiprophosmethyl (10, 15, or 20 μm) was applied to 27 microtillers for 3–5 days to induce chromosome doubling. All 14 surviving plants were tetraploid, (2n=4x=72), as determined by flow cytometry or chromosome counts. These plants were morphologically normal and produced seed. Test crosses were made with a known diploid. Flow cytometry and chromosome counts showed that the progeny were triploid, proving that the induced tetraploids reproduce sexually.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050502
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