ISSN:
1432-1440
Keywords:
Key words Porcine islets of Langerhans
;
Purification
;
Islet xenograft rejection
;
Islet transplantation
;
Islet culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Highly purified porcine islets were prepared by isokinetic gradients performed subsequently to isopycnic gradients. This additional purification step separates ductal, vascular, and lymphoid tissue effectively from endocrine tissue. Although ductal, vascular, and lymphoid tissue comprises only a minor contamination of the islet suspensions, a significant prolongation of the survival of porcine islets xenografted into streptozotocin diabetic C57BL/6 mice can be achieved by the elimination of the non-endocrine tissue. Rejection after islet transplantation is delayed from 2.2±0.4 days (n=27) to 13.1±2.1 days (n=36), respectiveley, when conventionally purified and highly purified islets are compared. Irrespective of the purification state, pretreatment of islets by low temperature culture had no effect on xenograft survival.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001090050331
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