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  • 1
    ISSN: 1432-0428
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Il a été démontré que l'albumine peut prévenir mieux que la gélatine la perte d'insuline d'une solution contenant de l'insuline. Ceci a probablement conduit à des rapports erronés de taux d'ILA élevés dans les dosages avec des méthodes biologiques. Des préparations d'albumine humaine cristalline ne montraient ni d'ILA, ni d'insuline décelable par la méthode radio-immunologique. L'albumine humaine préparée après extraction par l'alcool-acide (qui dans des expériences antérieures a démontré des propriétés anti-insuliniques avec l'hémidiaphragme isolé du rat) montre une ILA considérable avec le tissu adipeux isolé du rat et aussi de l'insuline qui peut être démontrée par la méthode radioimmunologique.
    Abstract: Zusammenfassung Es konnte gezeigt werden, daß Albumin stärker als Gelatine den Insulinverlust insulin-haltiger Lösungen verhindern kann. Das hat wahrscheinlich zu falsch erhöhten Spiegeln der insulinähnlichen Aktivität (ILA) geführt, über die bei Bestimmung mit biologischen Methoden berichtet wurde. Präparate kristallinen menschlichen Albumins zeigten keine ILA und auch kein mit der radioimmunologischen Methode bestimmbares Insulin. Menschliches durch Säurealkohol-extraktion gewonnenes Albumin (das nach früheren Untersuchungen insulinantagonistische Eigenschaften am isolierten Rattenhemidiaphragma hat) zeigte am isolierten Rattenfettgewebe eine deutliche ILA und auch mit der radioimmunologischen Methode bestimmbares Insulin.
    Notes: Summary Albumin has been found to be more effective than gelatine in preventing insulin loss from insulin containing solutions. It is probable that this has led to falsely elevated levels of insulin-like activity (ILA) reported with bio-assays. Preparations of crystalline human albumin have been found to be free from ILA and from insulin measured by the radio-immuno-assay. Other human albumin prepared by acid-ethanol extraction (that has been shown previously to contain insulin antagonistic properties on the isolated rat hemidiaphragm) has contained significant ILA on the isolated rat fat pad and also contained insulin demonstrable by radio-immuno-assay.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0428
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of plant growth regulation 14 (1995), S. 9-13 
    ISSN: 1435-8107
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Monosodium methanearsonate (MSMA) is an effective herbicide used for the control of Xanthium strumarium (cocklebur), a serious weed problem in cotton production. Recently, MSMA-resistant cocklebur was reported, which reduced MSMA effectiveness in the cocklebur control strategy. The mode of action of MSMA may involve the reduction of the pentavalent arsenic in MSMA to a trivalent form, which may then form a complex with sulfhydryl-containing enzymes. Dimercaptopro-panesulfonic acid (DMPS) chelates trivalent arsenic, resulting in a nontoxic complex. The objective of this study was to determine the effect of DMPS and MSMA on the growth of in vitro grown cocklebur shoot and callus tissues. Lateral and terminal shoots were grown on basal Murashige and Skoog medium containing 6.25 mg L−1 (0.0428 mm) MSMA alone and with various concentrations of DMPS. Callus was cultured on basal callus medium also containing 6.25 mg L−1 MSMA alone and an equimolar concentration of DMPS. Shoot injury symptoms, dry root weight, and fresh weight of callus were recorded. Susceptible cocklebur shoots showed severe discoloration and death when treated with MSMA. Resistant shoots and cultured shoot tips were not adversely affected. DMPS alone did not inhibit growth of these tissues. Callus induced from susceptible tissue was inhibited by MSMA and DMPS alone. In all combinations of DMPS and MSMA tested, DMPS did not counteract the MSMA response. Thus, the pentavalent form of arsenic in MSMA apparently is not reduced to the trivalent form by the plant and hence is not involved in the mechanism of action
    Type of Medium: Electronic Resource
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