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  • 1
    Electronic Resource
    Electronic Resource
    Polymorphism of insertion sites of Ty1-copia class retrotransposons and its use for linkage and diversity analysis in pea (1998)
    Springer
    Molecular genetics and genomics 260 (1998), S. 9-19 
    Details
    ISSN: 1617-4623
    Keywords: Key words Pea (Pisum) ; Ty1-copia retroelements ; Genetic diversity ; Linkage map ; Anchored PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A sample of 15 cultivars and 56 Pisum accessions from the JIC germplasm core collection has been studied using a modification of the SSAP (sequence-specific amplification polymorphisms) technique; the specific primer was designed to correspond to the polypurine tract (PPT) of PDR1, a Ty1-copia group retrotransposon of pea. Most of these SSAP products were shown to be PDR1 derived. The PDR1 SSAP markers are more informative than previously studied AFLP or RFLP markers and are distributed throughout the genome. Their pattern of variation makes them ideal for integrating genetic maps derived from related crosses. Data sets obtained with AFLP and PDR1 SSAP markers were used to construct neighbour-joining trees and for principal component analysis. These data sets give greater resolution than hitherto available for the characterisation of variation within Pisum, showing that the genus has three main groups: P. fulvum, P. abyssinicum and all other Pisum spp. P. abyssinicum is not a subgroup of cultivated P. sativum, as was previously thought, but has probably been domesticated independently. Modern cultivars are shown to form a single group within Pisum as a whole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008630
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic mapping and functional analysis of a nodulation-defective mutant (sym19) of pea (Pisum sativum L.) (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 1-11 
    Details
    ISSN: 1617-4623
    Keywords: Key words Root nodule ; ENOD12 ; Rhizobium ; Sym19 ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pea mutant line P55 is defective in root nodule formation, and this phenotype is controlled by a single recessive gene. Complementation analysis revealed that the mutation in P55 is allelic to sym19, which has previously been mapped to linkage group I. Detailed mapping revealed that the sym19 and ENOD40 loci are separated by 2.7 cM. We identified four recombination events, demonstrating that the nodulation defect caused by mutation of the sym19 locus cannot be due to mutation of ENOD40. RT-PCR experiments showed that P55 expresses ENOD12A, but there was little or no increase in the level of its transcript in response to Nod factor or infection with Rhizobium. To investigate this expression pattern further, transgenic peas carrying a pENOD12A-GUS reporter construct were made. One transgenic line was crossed with line P55, to generate F2 progeny homozygous for sym19 and carrying pENOD12A-GUS. In both WT and sym19 mutant lines, ENOD12A-GUS expression was induced at sites of lateral root emergence in uninoculated plants. In Nod+ plants pENOD12A-GUS was induced in response to Rhizobium leguminosarum bv. viciae, but no such induction was seen in the Nod− (sym19) mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051053
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  • 3
    Electronic Resource
    Electronic Resource
    Pea Ty1-copia group retrotransposons: transpositional activity and use as markers to study genetic diversity in Pisum (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 898-907 
    Details
    ISSN: 1617-4623
    Keywords: Key words Retrotransposon ; Genetic diversity ; Molecular taxonomy ; Pea ; DNA markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The variation in transposition history of different Ty1-copia group LTR retrotransposons in the species lineages of the Pisum genus has been investigated. A heterogeneous population of Ty1-copia elements was isolated by degenerate PCR and two of these (Tps12 and Tps19) were selected on the basis of their copy number and sequence conservation between closely related species for further in-depth study of their transpositional history in Pisum species. The insertional polymorphism of these elements and the previously characterised PDR1 element was studied by sequence-specific amplification polymorphism (SSAP). Each of these elements reveals a unique transpositional history within 55 diverse Pisum accessions. Phylogenetic trees based on the SSAP data show that SSAP markers for individual elements are able to resolve different species lineages within the Pisum genus. Finally, the SSAP data from all of these retrotransposon markers were combined to reveal a detailed picture of the intra and inter-species relationships within Pisum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380000257
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  • 4
    Electronic Resource
    Electronic Resource
    Heterogeneity of the internal structure of PDR1, a family of Ty1/copia-like retrotransposons in pea (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 703-713 
    Details
    ISSN: 1617-4623
    Keywords: Key words Retrotransposon rearrangements ; Pea ; Structural heterogeneity ; Deletion hot spot ; Biodiversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We characterised the extent of heterogeneity among PDR1 elements, a Ty1/copia-like retrotransposon family in pea, by restriction mapping and PCR with primers designed to amplify four functional domains. The data suggest that two main subfamilies of PDR1 differ in the size of their 5′-region. There are also sequence variants and rearranged copies which include a wide range of deletions of different sizes and deletions combined with insertions of host DNA, or inversions of various regions of the retrotransposon. A deletion hot-spot has been found at nucleotide position 394, where buffer sequences of 26 bp and 38 bp containing microsatellite motifs have been generated. There is more heterogeneity in the gag domain of PDR1 than in other functional domains, and the extent and pattern of this diversity was assessed among 56 Pisum accessions. We found a higher rate of rearrangement and sequence variation within the gag domain of PDR1 in P. fulvum and P. abyssinicum accessions than would be expected from the degree of insertion site polymorphism. A neighbour-joining phylogenetic tree constructed for gag sequences has a similar branching pattern to the equivalent insertion site tree, implying that the PDR1 family and its gag domain have coevolved with the pea genome. Combining both trees revealed clear and distinct subgroups among the Pisum ssp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051132
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  • 5
    Electronic Resource
    Electronic Resource
    The organization and genetics of rDNA length variants in peas (1984)
    Springer
    Chromosoma 91 (1984), S. 74-81 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract There is extensive repeat length variation in pea rDNA. Many lines of pea have more than one repeat length class, and those with two rDNA repeat length classes are the most common. No interspersion of rDNA repeat length classes has been found. The rDNA length classes show simple Mendelian inheritance. Length variation of pea rDNA is organized between long tandem arrays of rDNA repeat. Studies of trisomies have shown that rDNA length class dosage correlates with chromosome dosage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286487
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  • 6
    Electronic Resource
    Electronic Resource
    Restriction fragment length polymorphism markers in relation to quantitative characters (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 1-2 
    Details
    ISSN: 1432-2242
    Keywords: Quantitative characters ; Polymorphism ; Restriction fragment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple consideration of the potential of restriction fragment length polymorphism mapping, as a method of analysing the inheritance of quantitative characters, suggests that there are severe limitations to the utility of this approach.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261445
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  • 7
    Electronic Resource
    Electronic Resource
    An RFLP marker for rb in pea (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 362-365 
    Details
    ISSN: 1432-2242
    Keywords: Pea ; r b ; RFLP ; Vicilin gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have located an RFLP marker, corresponding to the locus Vc-5, which is linked to the r b locus. We also show that the heterogeneity at the Vc-5 locus is less among r brb lines than among pea genotypes as a whole. The relevance of this RFLP is discussed in relation to the construction of the double recessive rr r brb genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303978
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  • 8
    Electronic Resource
    Electronic Resource
    Comparative analysis of genetic diversity in pea assessed by RFLP- and PCR-based methods (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1103-1111 
    Details
    ISSN: 1432-2242
    Keywords: Key words Diversity ; Molecular-markers ; Pea (Pisum) ; Relatedness-trees ; Mantel's test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA-based molecular-marker techniques have been proven powerful in genetic diversity estimations. Among them, RFLP was the first and is still the most commonly used in the estimation of genetic diversity of eukaryotic species. The recently developed PCR-based multiple-loci marker techniques, which include RAPD, AFLP, Microsatellite-AFLP and inter-SSR PCR, are playing increasingly important roles in this type of research. Despite the wide application of these techniques, no direct comparison of these methods in the estimation of genetic diversity has been carried out. Here we report a direct comparison of DNA-based RFLP with various PCR-based techniques regarding their informativeness and applicability for genetic diversity analysis. Among ten pea genotypes studied, all the PCR-based methods were much more informative than cDNA-RFLP. Genetic diversity trees were derived from each marker technique, and compared using Mantel's test. By this criterion, all trees derived from the various molecular marker techniques, except for the tree derived from inter-SSR PCR, were significantly correlated, suggesting that these PCR-based techniques could replace RFLP in the estimation of genetic diversity. On the basis of this result, AFLP analysis was applied to assess the genetic diversity of a sample of accessions representing the various species and subspecies within the genus Pisum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050342
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Comparative analysis of genetic diversity in pea assessed by RFLP- and PCR-based methods (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1103-1111 
    Details
    ISSN: 1432-2242
    Keywords: Diversity ; Molecular-markers ; Pea (Pisum) ; Relatedness-trees ; Mantel's test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA-based molecular-marker techniques have been proven powerful in genetic diversity estimations. Among them, RFLP was the first and is still the most commonly used in the estimation of genetic diversity of eukaryotic species. The recently developed PCR-based multiple-loci marker techniques, which include RAPD, AFLP, Microsatellite-AFLP and inter-SSR PCR, are playing increasingly important roles in this type of research. Despite the wide application of these techniques, no direct comparison of these methods in the estimation of genetic diversity has been carried out. Here we report a direct comparison of DNA-based RFLP with various PCR-based techniques regarding their informativeness and applicability for genetic diversity analysis. Among ten pea genotypes studied, all the PCR-based methods were much more informative than cDNA-RFLP. Genetic diversity trees were derived from each marker technique, and compared using Mantel's test. By this criterion, all trees derived from the various molecular marker techniques, except for the tree derived from inter-SSR PCR, were significantly correlated, suggesting that these PCR-based techniques could replace RFLP in the estimation of genetic diversity. On the basis of this result, AFLP analysis was applied to assess the genetic diversity of a sample of accessions representing the various species and subspecies within the genus Pisum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230132
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  • 10
    Electronic Resource
    Electronic Resource
    AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 751-758 
    Details
    ISSN: 1432-2242
    Keywords: Lens ; AFLP ; RAPD ; Phylogeny ; Diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract AFLP and RAPD marker techniques have been used to evaluate and study the diversity and phylogeny of 54 lentil accessions representing six populations of cultivated lentil and its wild relatives. Four AFLP primer combinations revealed 23, 25, 52 and 48 AFLPs respectively, which were used to partition variation within and among Lens taxa. The results of AFLP analysis is compared to previous RAPD analysis of the same material. The two methods provide similar conclusions as far as the phylogeny of Lens is concerned. The AFLP technique detected a much higher level of polymorphyism than the RAPD analysis. The use of 148 AFLPs arising from four primer combinations was able to discriminate between genotypes which could not be distinguished using 88 RAPDs. The level of variation detected within the cultivated lentil with AFLP analysis indicates that it may be a more efficient marker technology than RAPD analysis for the construction of genetic linkage maps between carefully chosen cultivated lentil accessions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224072
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