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  • 1
    ISSN: 1432-1106
    Keywords: Cat ; Rat ; Spinal tract neurons ; Retrograde HRP transport ; Procedure and evaluation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Modifications have been made in Mesulam's method for labelling neurons by retrograde transport of horseradish peroxidase, with tetramethylbenzidine as chromogen, with the object of increasing the extent of labelling of dendrites and axons. A procedure was devised specifically for studying spinomedullary and medullospinal tract systems, involving implanting easily-made HRP-agar pellets into areas of controlled damage in particular spinal fascicles, and sealing the site of implant with cyanoacrylate glue. Lesions of other fascicles were often made to limit transport to the implanted fascicle. Fourth-order dendrites were regularly labelled over long (30 cm or more) transport distances: axons were also labelled over this whole distance, often allowing exact study of the initial course of particular axons. Controls in both cat and rat showed that the uptake of HRP under these circumstances occurred almost wholly from the region of axonal damage at the site of implant which can be characterized histologically.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 54 (1984), S. 538-550 
    ISSN: 1432-1106
    Keywords: Medullary nuclei ; Spinally-projecting cells ; Dendritic trees ; Axon trajectories ; Retrograde HRP transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The distribution, dendritic trees and axonal courses of spinally projecting cells in the dorsal column nuclei were studied after labelling by retrograde HRP transport. The region of densest distribution was at the base of the two nuclei and in the area between them, extending for about 2 mm caudally from the obex. Only very few cells were found inside the cell cluster regions of the nuclei, where their dendrites had a free stellate form. The great majority, lying between, deep, or rostral to the cluster regions, also had a stellate form, except where they impinged on the boundaries of the cluster regions or on other nuclear borders; the spread of dendrites was dramatically restricted at such boundaries, often leading to a fusiform appearance in transverse sections which however was not evident in the parasagittal plane. No justification was therefore found for subdividing the population on morphological grounds. Axons of these cells descended ipsilaterally in either the medial part of the dorsolateral fascicle or in the adjacent lateral part of the cuneate fascicle, at cervical levels, and probably in about equal numbers. Most axons destined for the DLF followed a deep caudolateral trajectory, while many destined for the DC had a more dorsal or lateral course. Collateral branches were seen within the nuclei but could not be followed far. The fact that few if any cells lying in the region of maximum distribution of the spinally projecting cells were labelled following injections of HRP into the thalamic ventroposterior nucleus emphasizes that they form a distinctive entity within this medullary nuclear complex, and that any axon branches they give into the contralateral brainstem must have some other destination than the VPL. Two other groups of neurons were labelled by HRP implants into the dorsal columns — one in the ventrolateral medullary reticular formation, and the other in the nucleus of the solitary tract.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1106
    Keywords: Dorsal horn ; Somaesthetic system ; Spinocervical tract ; Dorsolateral fascicle ; Dorsal column nuclei ; Retrograde HRP transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Spinocervical cells were identified by retrograde labelling from implants of HRP in the dorsolateral fascicle after destruction of the dorsal columns. They lay in laminae III and IV throughout the cord in estimated numbers of 700, 450 and 1100 in lumbosacral enlargement, upper lumbar and thoracic cord, and brachial enlargement respectively. In the cord enlargements dendritic trees were mainly or exclusively developed dorsally, with rostrocaudal exceeding mediolateral spread, and a gradient across the dorsal horn, lateral cells showing this contrast most strongly. Dendritic spread was limited at the II/III laminar boundary. Transition occurred at the edge of the enlargements to a shape with extreme rostrocaudal elongation of perikarya and of dendritic trees in upper lumbar and thoracic segments. Axons of Spinocervical cells ascended in the most dorsal part of the fascicle, distinguishable from the larger spinocerebellar bundle lying adjacent and ventral. The initial axonal course was tortuous, with local collateral branching, the axon sometimes travelling briefly in the dorsal column. In other experiments implants were made ipsilaterally in the dorsal column nuclei after destruction of the dorsal columns. Cells were few and relatively poorly labelled, for which the reasons are discussed. Some such cells, lying in lamina IV, were similar to spinocervical tract cells and may have projected to both lateral cervical and dorsal column nuclei. Others, at the extreme lateral edge of the mid-dorsal horn, were quite different, with dendrites greatly extended rostrocaudally and primary and higher order dendrites projecting ventrally from the perikaryon.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 75 (1989), S. 611-620 
    ISSN: 1432-1106
    Keywords: Dorsal column ; Somaesthetic system ; Spinal tract neurons ; Dorsal column ; Retrograde HRP transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The numbers, laminar position, perikaryal and dendritic morphology, and axonal trajectories of postsynaptic cells ascending the dorsal column have been studied after implantation of HRP pellets in either the dorsal columns or dorsal column nuclei after destruction of the dorsolateral fascicle on one side. Observations made throughout the spinal cord gave estimated figures of 800–1000 and 1700–2000 cells in lumbosacral and brachial enlargements respectively on the side of the implant. The commonest type (C), centred on lamina IV, had dendritic trees greatly extended rostrocaudally and restricted mediolaterally in the lateral dorsal horn, the extension and restriction diminishing for more medial cells. Type B cells differed dramatically, with large straight dendrites in the transverse plane and large perikarya in medial lamina V. Type A cells, distinguished by both rostrocaudal and mediolateral restriction in dendritic trees, were only found medially in laminae III and IV. Outside the enlargements, in high lumbar and thoracic cord, many fewer cells were found, corresponding to Type C but with dendrites much elongated rostrocaudally and little mediolateral variation. Many small fusiform cells were found in medial lamina VI in the upper cervical cord, distinct from any of the above. A few cells were found in the cord enlargements in lamina VII of the contralateral ventral horn, with axons crossing through the ventral commissure. The axons of all cell types were tortuous, and some entered the dorsolateral fascicle before crossing into the dorsal column: collaterals were often seen but could not be followed far. A complementary study of cells with axons ascending in the dorsolateral fascicle is reported in the following paper.
    Type of Medium: Electronic Resource
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