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  • 1
    Electronic Resource
    Electronic Resource
    Attempts to overcome postfertilization barrier in interspecific crosses of the genus Lens (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Wild relatives are a potential source of genetic diversity to lentil (Lens culinaris Medik). The objective of this research was to obtain viable interspecific hybrids between the domesticated lentil and its wild relatives.The paper details the results of a number of interspecific crosses among L. culinaris, L. orientalis, L. odemensis, L. ervoides and L. nigricans. Viable hybrids were produced between L. culinaris × L. orientalis, L. culinaris × L. nigricans, L. culinaris × L. ervoides and between L. culinaris × L. odemensis. Further viable hybrids were obtained between L. culinaris and L. ervoides, which have the potential to be a ‘bridge’ in hybridization to L. culinaris for specific L. nicrigans lines which proved recalcitrant in L. culinaris × L. nigricans crosses. This is the first time that four wild species of lentils have been used successfully in hybridization with cultivated lentils, and viable hybrids produced. This paper also suggests that the artificial supplement of GA3, hormone is needed after fertilization for the normal growth of the hybrid embryo, possibly as the natural GA3 production is restricted with alien pollinations in cultivated lentils in both F1 and backcross hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00859.x
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  • 2
    Electronic Resource
    Electronic Resource
    Morphological and molecular analysis of androgenetic, selfed and backcrossed plants produced from a Hordeum vulgare x H, bulbosum hybrid (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 116 (1997), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Anther culture (AC) was carried out on a fertile triploid hybrid between Hordeum vulgare L. (cultivated barley) and H. bulbosum L, (bulbous barley grass) to determine whether AC-derived regenerants differed from progeny obtained through selfing and backcrossing. Chromosome counts were carried out on all plants and DNA was extracted from them to prepare Southern blots for molecular analysis. To identify true recombinants, the blots were probed with rye repetitive sequence probes (pSc119.1 and pScl19.2). which hybridize strongly and specifically to H. bulbosum DNA. Twenty probes that detect single- or low-copy sequences were hybridized with Southern blots containing restricted DNA extracted from 25 AC-derived plants, 11 selfed and six backcrossed progeny that showed hybridizations with pScll9. Although restriction fragment length polymorphisms (RFLPs) were only observed using probes that map to four of the possible 14 chromosome arms, an introgression associated with chromosome 6HS was frequently observed among plants derived from AC. selfing and backcrossing. Plants from AC differed from selfed and backcrossed progeny in their chromosome number; unique RFLP bands that were occasionally observed may indicate chromosomal rearrangements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1997.tb02181.x
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  • 3
    Electronic Resource
    Electronic Resource
    Anther Culture-Derived Regenerants from Hordeum vulgare × H. bulbosum Crosses (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 110 (1993), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Hordeum bulbosum has several desirable attributes, including disease resistance, which would be worthwhile transferring to H. vulgare. Despite homoeologous chromosome pairing in the interspecific hybrids, there have been few reports of successful gene introgression between the two species. A possible explanation for this is that recombinant male gametes are at a competitive disadvantage with normal balanced gametes during post-pollination events. To circumvent this problem, the possibility of obtaining plants directly from immature pollen grains was investigated. Anthers from diploid, triploid and tetraploid H. vulgare × H. bulbosum hybrids were cultured on defined media. Only hybrids with dehiscent anthers in vivo responded in culture, and after transfer of calli and embryoids to regeneration medium, 36 albino and 12 green plants were obtained. Seven of the green regenerants survived, one of which contained 15 H. vulgare chromosomes (including one acrocentric chromosome) and one H. bulbosum chromosome. Another regenerant (Ac166) resembled a diploid H. vulgare × H. bulbosum hybrid but had partial anther dehiscence and a slightly modified chromosome constitution. Mostly normal H. vulgare progeny were obtained from crosses between H. vulgare cv.‘Emir’ and Ac166, but three plants involved chromosome additions and substitutions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1993.tb00567.x
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic relationships in Lens species and parentage determination of their interspecific hybrids using RAPD markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 1091-1098 
    Details
    ISSN: 1432-2242
    Keywords: Lens culinaris ; Wild species ; RAPD ; Intra- and interspecific variation ; Genetic distance ; Split decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Broadening of the genetic base and systematic exploitation of heterosis in cultivated lentils requires reliable information on genetic diversity in the germplasm. The ability of random amplified polymorphic DNA (RAPD) to distinguish among different taxa of Lens was evaluated for several geographically dispersed accessions/cultivars of four diploid Lens species. This study was carried out to assess whether RAPD data can provide additional evidence about the origin of the cultivated lentil and to measure genetic variability in lentil germplasm. Three cultivars of Lens culinaris ssp. culinaris, including one microsperma, and two macrosperma types, and four wild species (L. culinaris ssp. orientalis, L. odemensis and L. nigricans) were evaluated for genetic variability using a set of 1 11-mer and 14 random 10-mer primers. One hundred and fifty-eight reproducible and scorable DNA bands were observed from these primers. Genetic distances between each of the accessions were calculated from simple matching coefficients. Split decomposition analysis of the RAPD data allowed construction of an unrooted tree. This study revealed that (1) the level of intraspecific genetic variation in cultivated lentils is narrower than that in some wild species. (2) L. culinaris ssp. orientalis is the most likely candidate as a progenitor of the cultivated species, (3) L. nigricans accession W6 3222 (unknown) and L. c. ssp. orientalis W6 3244 (Turkey) can be reclassified as species of L. odemensis and (4) transmission of genetic material in Lens interspecific hybrids is genotypically specific, as identified by the RAPD markers in our study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224054
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  • 5
    Electronic Resource
    Electronic Resource
    IBPGR morphological descriptors — their relevance in determining patterns within a diverse spring barley germplasm collection (1992)
    Springer
    Theoretical and applied genetics 85 (1992), S. 489-495 
    Details
    ISSN: 1432-2242
    Keywords: IBPGR ; Morphological descriptors ; Genetic diversity ; Barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The International Board for Plant Genetic Resources (IBPGR) promotes a minimum set of morphological characters thought satisfactory for the custodial management of crop germplasm collections. The purpose of such conserved germplasm is as a genetic resource for future plant breeding programmes. Because future plant breeding requirements are not always known, the curator's strategy in maintaining an adequate germplasm resource is to conserve as wide a range of genetic diversity as possible. How is diversity measured to ensure a wide range of conserved germplasm? The IBPGR minimum descriptors detail genetic diversity at particular points in a genome corresponding to the observed characters. The purpose of the present study was to investigate whether diversity as identified by the IBPGR minimum set of descriptors could yield satisfactory measures of diversity in a contrasting set of genomic markers. A diverse spring-sown barley collection of 1379 cultivars was evaluated for the 12 IBPGR minimum taxonomic characters. An additional nine phenotypic characters and six biochemical markers were evaluated to enable diversity comparisons. Cluster analysis of the various sets of data revealed groups of accessions for each of the three data sets. A poor level of agreement (congruence) between data sets was observed in all comparisons indicating that, for cultivated barley at least, diverse collections according to the IBPGR minimum descriptors is not necessarily related to equivalent levels of diversity in other genetic characters. Implications of the relevance of the IBPGR descriptor list and appropriate collection strategies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222331
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic relationships in Lens species and parentage determination of their interspecific hybrids using RAPD markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 1091-1098 
    Details
    ISSN: 1432-2242
    Keywords: Key words Lens culinaris ; Wild species ; RAPD ; Intra- and interspecific variation ; Genetic distance ; Split decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Broadening of the genetic base and systematic exploitation of heterosis in cultivated lentils requires reliable information on genetic diversity in the germ-plasm. The ability of random amplified polymorphic DNA (RAPD) to distinguish among different taxa of Lens was evaluated for several geographically dispersed accessions/cultivars of four diploid Lens species. This study was carried out to assess whether RAPD data can provide additional evidence about the origin of the cultivated lentil and to measure genetic variability in lentil germplasm. Three cultivars of Lens culinaris ssp. culinaris, including one microsperma, and two macrosperma types, and four wild species (L. culinaris ssp. orientalis, L. odemensis and L. nigricans) were evaluated for genetic variability using a set of 1 11-mer and 14 random 10-mer primers. One hundred and fifty-eight reproducible and scorable DNA bands were observed from these primers. Genetic distances between each of the accessions were calculated from simple matching coefficients. Split decomposition analysis of the RAPD data allowed construction of an unrooted tree. This study revealed that (1) the level of intraspecific genetic variation in cultivated lentils is narrower than that in some wild species. (2) L. culinaris ssp. orientalis is the most likely candidate as a progenitor of the cultivated species, (3) L. nigricans accession W6 3222 (unknown) and L. c. ssp. orientalis W6 3244 (Turkey) can be reclassified as species of L. odemensis and (4) transmission of genetic material in Lens interspecific hybrids is genotypically specific, as identified by the RAPD markers in our study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050235
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    In vitro propagation ofLens species and their F1 interspecific hybrids (1997)
    Springer
    Plant cell, tissue and organ culture 47 (1997), S. 169-176 
    Details
    ISSN: 1573-5044
    Keywords: Lentils ; wild relatives ; micropropagation ; media optimization ; growth regulators ; nodal segments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract As an initial step in establishing interspecific hybridization to broaden the genetic basis of lentils [Lens culinaris ssp.culinaris (Medikus) Williams], a set of experiments was carried out to produce an efficient in vitro protocol for propagation of lentil and two of its wild relatives (Lens ervoides andLens culinaris ssp.orientalis). The objective of the experiments was to optimize the media (Murashige and Skoog) to regenerate shootsin vitro from nodal segments without a callogenic phase. The number of shoots per explant, the number of nodes per shoot and shoot length showed that species differences, gibberellic acid and benzyladenine levels had the largest effects, with only minor interaction effects. The experiments therefore identified a standard protocol which gave the optimum levels of growth regulators, Murashige and Skoog (MS) salts and sucrose concentrations for maximum plant regeneration from the nodal segment of these species. The medium recommended for optimal shoot regeneration without a callogenic stage contained 2.89 μM GA3 in combination with 1.11 μM BA in MS medium lacking sucrose. The optimal medium for root induction on these shoots had the MS medium supplemented with 5.37 μM NAA. Final successful establishment of regenerated plants was completed by the transfer to a third medium containing half-strength MS salts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02318954
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