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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 206 (1998), S. 308-310 
    ISSN: 1431-4630
    Keywords: Key words Galician chorizos ; Sensory ; Olive oil ; Sunflower oil ; Pork fat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Changes in the physicochemical and sensory properties of Galician chorizos stored in olive oil, sunflower oil or pork fat were monitored over a 19-week period. The results of analysis of variance showed that the storage medium had a significant effect on some variables. Specifically, moisture content, water activity, acid value and cohesiveness were lowest in chorizos stored in sunflower oil; acid value and pH were highest in chorizos stored in olive oil; external smoky and paprika odours were lowest, and after-taste intensity highest, in chorizos stored in pork fat. Acid value, peroxide index, hardness, colour, external odour intensity, perceived hardness and acid flavour all increased significantly during storage, while pH and water activity declined. In all three storage media, external rancid odour and rancid after-taste were first detected at week 17, when the peroxide index peaked. The overall rating was best for chorizos stored in sunflower oil.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 207 (1998), S. 18-21 
    ISSN: 1431-4630
    Keywords: Key words Galician chorizo sausage ; Colour ; CIE Lab.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Galician chorizo sausage is a smoked-meat product containing chilli pepper. This study investigated changes in its colour (CIE colour coordinates L*, a* and b*, together with redness index, hue, chroma and perceived colour) during the curing process. Samples were obtained from three manufacturers (A, B and C) whose smoking and curing procedures differ markedly. Most of the colour measurement showed clear changes during curing. All measurement except external L* varied significantly among samples obtained from the different manufacturers. Pairwise comparisons indicated that in most cases there were significant differences between samples from manufacturer A (who smokes the product for only 6 h) and manufacturers B and C (who smoke the product for a longer period), but not between samples from manufacturers B and C. The pH, water activity and moisture content were also monitored; again, these variables differed significantly between A and B and between A and C, but not between B and C.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 83 (1992), S. 543-546 
    ISSN: 1432-0533
    Keywords: Giant axonal neuropathy ; Intermediate filaments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We report on a 5 1/2 year-old boy with chronic progressive polynèuropathy, ataxia, and pyramidal signs. His hair was not curled. Sural nerve biopsy disclosed many axons enlarged by accumulation of 10-nm neurofilaments and a marked variability in the number of myelinated fibers as well as in the amount of axonal enlargements among different fascicles. These findings and the electrophysiological data were consistent with a giant axonal polyneuropathy with a multifocal fiber loss.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 129 (1997), S. 399-406 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The storage of nitrate by phytoplankton cells during the early phases of upwelling was studied in coastal stations off northern Spain (southern Bay of Biscay) between 1990 and 1994. In this region, a persistent upwelling during summer is characterised by intermittent pulses of variable intensity, and increased nutrient concentrations in the surface layer. The main effect of an upwelling pulse on phytoplankton distribution is the shifting of the chlorophyll a and primary production maxima to near the surface. When the upwelling relaxes, thermal stratification of the water column occurs, and a distinct subsurface chlorophyll maximum develops below the production maximum. An accumulation of intracellular nitrate characterized the early phases of upwelling (mean = 2.73 μmol N m−3), maximum concentrations being attained at depths where biomass and production values were moderate. In contrast, phytoplankton cells from non-upwelling situations contained significantly lower concentrations of intracellular nitrate (mean = 0.17 μmol N m−3). The variations in the intracellular pool of nitrate may result from the differential allocation of resources within the cell as a result of variations in the energy available, since the uptake and assimilation of nitrate is a relatively expensive process involving several enzymatic systems. We hypothesize that nitrate storage by phytoplankton cells is characteristic of early phases of upwelling and is linked to patterns of carbon fixation. Average nitrogen budgets for upwelling and non-upwelling situations indicate that intracellular nitrate reserves are not responsible for maintaining high phytoplankton growth rates, since they only account for 〈2% of daily primary production during upwelling events.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Particle-size distributions and several biochemical components of seston were studied from October 1986 to December 1987 in surface waters of the Bay of Biscay. Variance partitioning of hydrographic and seston parameters indicates that, although seasonal variability related to the thermal cycle was important, most of the differences in seston concentration were caused by spatial components of variance. Changes in the vertical structure of the water column appeared to be the principal source of variability. Phytoplankton blooms in spring and fall and the effects of the coastal upwelling and the thermocline during summer were traced using seston concentration and biochemical composition. The different seston measurements were scaled according to their correlations with total concentration and particle size. Photosynthetic pigment biomass was related to both large and small partieles. However, concentrations of particulate proteins, lipids and carbohydrates were more related to small particles. Although not specifically analysed, low concentrations of inorganic particles and detritus can be expected in the study area, thus most of the particulate organic matter was associated with small, non-pigmented organisms, particularly in surface waters during the period of thermal stratification. The variability in concentrations of total seston and particulate organic matter was mainly due to variations in particulate proteins, lipids and carbohydrates; pigment concentrations were of secondary importance. Expressed as the ratio chlorophyll a: particulate protein-nitrogen, phytoplankton constituted an important fraction of the microplankton biomass only during spring blooms, when it averaged 75% of the particulate protein-nitrogen. In constrast, 〈30% of protein-nitrogen was related to chlorophyll a during the summer. These results suggest that an oligotrophic structure based on the “microbial-loop” prevails in microplankton assemblages of surface waters for most of the year.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We investigated the influence of bacteria and metazooplankton on the production of dimethylsulfoniopropionate (DMSP) and dimethylsulfide (DMS) during blooms of Emiliania huxleyi (Lohmann) Hay and Mohler in seawater mesocosms. The phytoplankton succession was marked by the rapid collapse of an initial Skeletonema costatum (Greville) Cleve bloom followed by a small E. huxleyi bloom. The collapse of the diatom bloom was accompanied by an increase in concentrations of dissolved DMSP (DMSPd) and bacterial abundance and activity (as determined by the thymidine incorporation technique). The increase in bacterial activity was followed by a rapid decrease in DMSPd concentrations which remained low for the rest of the experiment, even during the subsequent collapse of the E. huxleyi blooms. The absence of DMSPd and DMS peaks during the declining phase of the E. huxleyi blooms was attributed to the high bacterial activity prevailing at that time. The influence of metazooplankton grazing on DMSP and DMS production was investigated by adding moderate (24 mg dry weight m-3) and high (520 mg dry weight m-3) concentrations of Copepodite Stage V and adults of Calanus finmarchicus to two of four filtered (200 μm mesh net) enclosures during the E. huxleyi blooms. The addition of C. finmarchicus, even in high concentrations, had no apparent effect on the dynamics of E. huxleyi, suggesting that the copepods were not grazing significantly on nanophytoplankton. The addition of copepods in high concentrations favored an accumulation of chlorophyll a and particulate DMSP. These results suggest that copepods were preying on the herbivorous microzooplankton which, in turn, was controlling the biomass of nanophytoplankton. DMS production was also enhanced in the enclosure with maximum metazooplankton biomass, suggesting that the grazing of C. finmarchicus on microzooplankton containing DMSP may contribute to DMS production. These results provide strong support to the emerging idea that bacteria and metazooplankton grazing play a dominant role in determining the timing and magnitude of DMS pulses following phytoplankton blooms.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Huntington's disease (HD) is a neurodegenerative and hereditary disease characterized by progressive movement disorders and mental and behavioral abnormalities. The HD gene is an expanding and unstable trinucleotide repeat (CAG repeat sequences). We studied 77 individuals from 38 families with HD in an attempt to obtain information for genetic counselling and differential diagnosis. Our results indicate that individuals with more than 40 repeats will be affected by the disease, whereas those with fewer than 30 will be healthy. There can be some overlap between 30 and 40 repeats, and one should be careful when interpreting these results.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Spanish cystic fibrosis (CF) families (n = 194) have been analysed for the ΔF508 mutation, and for closely linked DNA markers. The ΔF508 mutation accounts for 50% of CF chromosomes. Four haplotypes are associated with the deletion, and at least seven haplotypes carry other mutations. The second major CF mutation is associated with pancreatic insufficiency and occurred in the same haplotype in which the ΔF508 arose. Only 31% of Spanish CF patients with no family history of the disease can be accurately diagnosed; about 50% of CF carriers can be detected in the Spanish population.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-1238
    Keywords: Key words Severe trauma ; Computed tomography ; Cost minimization study ; Abdominal injury ; Chest trauma ; Trauma evaluation ; Trauma costs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objective: To compare contrast computed tomography (CT) for evaluating abdominal and vascular chest injuries after emergency room resuscitation with multidisciplinary management based on bedside procedure (BP), e.g., peritoneal lavage, abdomen ultrasonography urography and, if indicated, CT and/or aortography or transesophageal echocardiography. Design: Randomized study. Setting: Emergency, critical care and radiology departments in a trauma center. Patients: The study was performed in 103 severe blunt trauma patients with a revised trauma index 〈8, admitted over a 16 month period and divided into group 1 (G1, n=52, CT management) and group 2 (G2, n=51, BP management). Interventions: A relative direct cost scale used in our trauma center was applied, and cost units (U) were assigned to each diagnostic test for cost-minimization analysis (abdomen ultrasonograph=7.5 U, peritoneal lavage=8 U, urography=9 U, computed tomography=9 U, transesophageal echocardiography=13.5 U, and aortography=15 U). One unit is approximately equivalent to $ 43.7. Results: Injury severity score (ISS) was 31.7±15.4 in G1 and 33.8±18.3 in G2. Sensitivity for CT was 90.4% (G1) vs 72.5% for BP (G2) in abdomen (P〈0.01) and 60% in chest for evaluating mediastinal hematoma etiology (G1). As Table 2 shows, G1 needed 59 tests for evaluating injuries (1.1±0.3 tests patient) while G2 required 81 tests (1.68±0.8 tests/patient) (P〈0.01). The total relative cost was 538 U for G1, 7.04±2.2 U cost/injury and 10.3±3.3 U/evaluation of trauma vs 698 U for G2, 9.84±5.03 U cost/injury and 13.68±8.5 U/evaluation (P〈0.05). Conclusions: This cost-minimization study suggests that CT is a more cost-effective method for the post-emergency room resuscitation evaluation of severe abdominal blunt trauma than the multidisciplinary BP. Chest CT is a screening method for mediastinal hematoma but not for etiology.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1238
    Keywords: Severe trauma ; Computed tomography ; Cost minimization study ; Abdominal injury ; Chest trauma ; Trauma evaluation ; Trauma costs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objective To compare contrast computed tomography (CT) for evaluating abdominal and vascular chest injuries after emergency room resuscitation with multidisciplinary management based on bedside procedure (BP), e.g., peritoneal lavage, abdomen ultrasonography urography and, if indicated, CT and/or aortography or transesophageal echocardiography. Design Randomized study.Setting: Emergency, critical care and radiology departments in a trauma center. Patients The study was performed in 103 severe blunt trauma patients with a revised trauma index〈8, admitted over a 16 month period and divided into group 1 (G 1,n=52, CT management) and group 2 (G 2,n=51, BP management). Interventions A relative direct cost scale used in our trauma center was applied, and cost units (U) were assigned to each diagnostic test for cost-minimization analysis (abdomen ultrasonograph=7.5 U, peritoneal lavage=8 U, urography=9 U, computed tomography=9 U, transesophageal echocardiography=13.5 U, and aortography=15 U). One unit is approximately equivalent to $ 43.7. Results Injury severity score (ISS) was 31.7±15.4 in G1 and 33.8±18.3 in G2. Sensitivity for CT was 90.4% (G1) vs 72.5% for BP (G2) in abdomen (P〈0.01) and 60% in chest for evaluating mediastinal hematoma etiology (G1). As Table 2 shows, G1 needed 59 tests for evaluating injuries (1.1±0.3 tests patient) while G2 required 81 tests (1.68±0.8 tests/patient) (P〈0.01). The total relative cost was 538 U for G1, 7.04±2.2 U cost/injury and 10.3±3.3 U/evaluation of trauma vs 698 U for G2, 9.84±5.03 U cost/injury and 13.68±8.5 U/evaluation (P〈0.05). Conclusions This cost-minimization study suggests that CT is a more cost-effective method for the post-emergency room resuscitation evaluation of severe abdominal blunt trauma than the multidisciplinary BP. Chest CT is a screening method for mediastinal hematoma but not for etiology.
    Type of Medium: Electronic Resource
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