Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Comparative radioautographic study of the effects of L-azetidine-2-carboxylic acid on matrix secretion and golgi of the mouse incisor (1984)
    Springer
    Calcified tissue international 36 (1984), S. 409-420 
    Details
    ISSN: 1432-0827
    Keywords: LACA ; Odontoblasts and ameloblasts ; 3H-glycine ; Collagen secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The effect of a proline analog,l-azetidine-2-carboxylic acid (LACA), on protein matrix secretion by odontoblasts and ameloblasts was compared by light and electron microscopic radioautography after injection of3H-glycine in young mice. LACA inhibited the secretion of dentin matrix with consequent accumulation of3H-glycine labeled procollagen in the cisternae of the rough endoplasmic reticulum. In contrast, LACA had no apparent effect on ameloblasts as enamel matrix continued to be packaged in the Golgi apparatus and secreted from Tomes' process within 30 min after injection of the radioprecursor. Electron microscopy revealed that LACA did not cause any change in ameloblast ultrastructure but produced a marked alteration of the odontoblast Golgi complex. All odontoblast Golgi saccules and collagen secretion granules disappeared within 2 h after LACA administration. Odontoblast Golgi cisternae, however, appeared not to be affected. These observations confirm previous studies conducted in this laboratory showing that Golgi saccules in collagen-secreting cells are the initial staging areas for the formation of secretory granules. These results also indicate that a close correlation exists between form and function in the Golgi apparatus of collagen-secreting cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405353
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural evidence of directed cell migration during initial cementoblast differentiation in root formation (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 23 (1988), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Root formation in 14-day-old Sprague-Dawley rats was studied by light and electron microscopy. Special attention was focused on initial cementoblast differentiation. Disruption of the epithelial root sheath appears to be a consequence of directed cell migration by cells of the dental follicle proper which undergo differentiation into precementoblasts. Precementoblasts rapidly develop polarity towards the dentin, exhibiting major cytoplasmic processes rich in cytoplasmic filaments. These processes grow toward and eventually contact the dentin matrix. It is suggested that the cells of the dental follicle proper are cementoblast precursors which respond to chemoattractant substances released from newly deposited dentin matrix- and/or basal lamina-associated material of root sheath origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1988.tb01371.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The effect of beta-aminoproprionitrile on the periodontal ligament (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 19 (1984), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Administration of Beta-aminoproprionitrile (B-APN) to young mice produced a characteristic lesion, the lathyritic body, in the middle region of the PDL. Within these lesions the fibroblasts aggregated to form cell clusters of 10 to 20 cells. The aggregated fibroblasts bordered cell-free regions of collagenous matrix. Individual fibroblasts maintained cytoplasmic polarity and provided a good model for studying collagen secretion from immobilized fibroblasts.Collagenous matrix appeared to be resorbed in the immediate vicinity of the clustered fibroblasts. 3H-proline radioautography indicated that labelled collagenous matrix was preferentially lost from the middle region of the PDL of both control and experimental mice. However, the decrease in collagen in the middle region was accentuated by B-APN treatment and the aggregation of fibroblasts in that zone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1984.tb00816.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Cytoplasmic polarization of periodontal ligament fibroblasts (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 14 (1979), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The fibroblasts of the periodontal ligament and especially those of the transseptal ligament are characteristically elongated with their long axes parallel to the principal fiber bundles. Their cytoplasmic orgamelles are polarized suggesting that secretory products are released from one end of the cell. The nucleus occupies the anterior pole of the cell. Cytoplasmic processes or lobopodia, containing numerous cytoplasmic filaments, extend from the anterior pole of the cell. The Golgi complex is situated in a large area of the cell between the nucleus and the distal end of the cell. Granular endoplasmic reticulum, mitochondria and numerous small vesicles are dispersed throughout most part of the cell. Intracellular collagen, akthough present in many areas of the cell, appears to be most abundant in the distal parts of the cell. Administration of colchicine in vivo, blocked collagen secretion and dereased cell elongation and polartity.Results of and analysis of the orientation and polarization of the fibroblasts in the periodontal tissues appear to support the concept that these cells undergo active migration in vivo along existing substrata and that migration may be coupled to collagen secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1979.tb00778.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Effects of colchicine on periodontal ligament fibroblasts of the mouse (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 17 (1982), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effects of colchicine administration on the ultrastructure aad function of periodontal ligament fibroblasts was studied in young mice as a further attempt to document the microtubule-dependent nature of collagen secretory granule translocation.Disruption of the normal appearance of the Golgi complex was an early response to colchicine administration. This change was visible at the light microscopic level. At the electron microscopic level it was observed that Golgi saccules were dispersed and that Golgi cisternae were shorter and reduced in number. Microtubules were absent. Dense granules accumulated in a juxtanuclear region of cytoplasm previously occupied by the normal Golgi complex. Lipid droplets and autophagosomes were increased in number. Intermediate filaments (10 nm diameter) formed perinuclear fascicles several hours after colchicine treatment.These results as well as the recently published report (Cho & Garant 1981d) support the concept that microtubules play an important role in the normal organization of the Golgi complex and in the translocation of secretory granules to the fibroblast cell surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1982.tb01170.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Multinucleated fibroblastic cells in the periodontal ligaments of aged rats (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 28 (1993), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Using 12- to 18-month -old rats, we examined the ultrastructural and cytochemical features of multinucleated fibroblastic cells (MFCs) in the periodontal ligament (PDL) of molars. In aged rats, the MFCs were distributed randomly in the PDL and exhibted cytoplasmic structural variations which were not dependent on the number of nuclei. There was a tendency for the MFCs to cluster in the PDL. The MFCs, rich in cytoplasmic organelles involved with procollagen synthesis such a rought endoplasmic reticulum and the Golgi apparatus, incorporated and secreated 3H-prolince-labled products. The MFcs also possessed many phagosomes containing intact collagen fibrils.n These MFCs were apparently involved in phagocytosis and intracellular degradation of incorporated collagen fibrils. Phagosome-rich MFCs contain acid phosphatase activity in primary and secondary lysosomes, similar ro stronger in intensity to that which can be demonstrated in mononuclear fibroblasts. However, unlike mononuclear fibroblasts, the MFcs did not exhibit alkaline phosphatase acitivity along their plasma membranes. These results suggest that MFcs demonstrate a range of fibroblastic cellular activity, including collagen phagocytosis, and that they may lack certain plasma membrane glycoproteins, which might explain the occurrence of multinucleation in these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1993.tb01052.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of extracellular matrix components in beagle periodontium: I. Collagen types I and III in healthy gingival connective tissue (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 22 (1987), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The localization and identification of types I and III collagen in healthy gingival connective tissue of the beagle dog were investigated using indirect immunofluore-scent and immunoferritin electron microscopic techniques with affinity purified antibody or IgG. Type I collagen was evenly distributed throughout the lamina propria and the underlying connective tissue in the form of thick collagen fibers. In the lamina propria, these fibers appeared to run from the underlying connective tissue in a perpendicular orientation to the overlying gingival epithelium. Localization of type III collagen was restricted to the lamina propria and the connective tissue around blood vessels. Immunoferritin electron microscopy clearly revealed that type I collagen fibrils have a diameter ranging from 40 to 100 nm and a clear 640 Å cross-banding pattern. Thin and short collagen fibrils, 18 to 32 nm in diameter, were labelled with antitype III collagen IgG. A 640 Å cross-banding pattern was rarely observed in these fibrils. Type III collagen fibrils coexisted with type I collagen fibrils in a mixed pattern within the lamina propria and the perivascular space.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1987.tb01591.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Autoradiographic evidence of the coordinaion of the genesis of Sharpey'a fibers with new bone formation in the periodontium of the mouse (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 14 (1979), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Multiple injections of 3H-proline over a period of ten days produced a series of radiolabelled appositional bands at sites of new bone formation. Appositional surfaces of periodontal alveolar bone not only contained parallel appositional bands but also a second series of bands oriented perpendicular to the appositional bands. The perpendicular bands were continuous with Sharpey's fibers of the adjacent periodontal ligament (PDL). Labelling over Sharpey's fibers frequently extended beyond the osteoblasts and into the PDL. This pattern of 3H-Proline labelling suggests that new Sharpey's fiber are secreted simultaneouly with new bone deposition. The shape and polarity of adjacent PDL fibroblasts suggests that they may migrate away from the bone surface as they engage in Sharpey's fiber formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1979.tb00779.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    A freeze-fracture study of the periodontal ligament of the rat (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 18 (1983), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Fibroblasts of the rat periodontal ligament were studied in situ by the freeze-fracture technique. Large surfaces of the protoplasmic and extracellular faces of the cell membrane as well as, the membranes of the RER, Golgi apparatus, and other cytoplasmic organelles were viewed at the ultrastructural level. Intramembrane particles representing membrane proteins were visualized, and their concentration in various membranes measured.Features of the fibroblast cell surface appearing as rough patches were interpreted to represent areas of possible cell-to-matrix interaction. The use of the freeze-fracture technique in further studies of PDL fibroblast-matrix contact is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1983.tb00359.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Attachment of periodontal ligament fibroblasts to the extracellular matrix in the squirrel monkey (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 17 (1982), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Fibroblasts of the transseptal fiber region of the periodontal ligament contain well developed bundles of microfilaments or stress fibers. The stress fibers are made up of numerous parallel and closely packed cytoplasmic filaments approximately 6 nm wide. These intra-cellular stress fibers appear to be connected to and colinear with extracellular non-striated microfibrils. The junction occurs at a juxta-membrane dense patch called the fibronexus (Singer 1979).Similar associations of cytoplasmic microfilament bundles and the attachment protein fibronectin have been shown by other investigators to be necessary for cell attachment to substrata and oriented cell migration in fibroblasts cultured in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1982.tb01132.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...