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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Biochemical and Biophysical Methods 7 (1983), S. 255-261 
    ISSN: 0165-022X
    Keywords: Fluorescence Correlation Spectroscopy ; instrumental distortions ; photon statistics
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    European journal of soil science 47 (1996), S. 0 
    ISSN: 1365-2389
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Both P and Al MAS NMR spectra of samples of excessively fertilized sandy soil provided information about the P and Al speciation. Peak deconvolution was used to interpret reliably and quantitatively the 31P NMR spectra recorded. Most of the P was found to be associated with Al. Part of the P exhibited a chemical shift that could be attributed to octocalcium phosphate, amorphous calcium phosphate or apatite. Apatite has, however, never been reported to occur in sandy soils of temperate climates. A dithionite extraction used to remove interfering Fe from the samples also removed most of the octahedral Al-P phase. After oxalate extraction more than 99% of the original P signal disappeared. About 7.5 to 11 % of the total oxalate extractable P of the excessively fertilized soil was present as a Ca-P phase, even though these soils are slightly acid to acid. The estimated size of the Ca-P phase roughly corresponds to the size of the labile P pool of these soils, as assessed in long-term batch desorption experiments. It still remains unclear whether the labile P pool should be attributed solely to such a Ca-P phase.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Cell Biology International Reports 14 (1990), S. 38 
    ISSN: 0309-1651
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1572-879X
    Keywords: VPI-5 ; isomorphic substitution ; decane test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Si-VPI-5 crystals containing Si atoms in Si(4Al) environments arc synthesized. The crystals contain occluded amine. It is protonated and neutralizes the residual negative lattice charges associated with the Si(4A1) sites. Calcined Si-VPI-5 is active in the isomerisation of decane and exhibits extra-large pore characteristics.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of statistical physics 28 (1982), S. 173-176 
    ISSN: 1572-9613
    Keywords: Fluorescence correlation spectroscopy ; Smoluchowski process ; diffusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Fluorescence Correlation Spectroscopy (hereafter called FCS) is a technique used to determine the diffusion coefficientD by following the number of fluorescent molecules contained in a given spot at various times. We derive an expression, relating the variance of the estimatedD to several experimental parameters. The main results give a qualitative description of how the statistical uncertainty of this inherently stochastic technique depends on adjustable variables, such as duration of the experiment, maximal autocorrelation lag, beam size, etc.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 9 (1988), S. 30-47 
    ISSN: 0886-1544
    Keywords: video microscopy ; colloidal gold ; microtubules ; saltatory movement ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Transferrin receptors labeled with the B3/25 monoclonal antibody-gold complexes were followed in living A431 cells by using video-enhanced contrast microscopy. Initially, the antibody-gold complexes bind to receptors which are freely mobile on the upper cell surface; they then become trapped at the inner margins of the peripheral lamellae and internalize. During endocytosis discrete gold-loaded vesicular elements first appear, and then, as they fuse, a heterogenous peripheral endosomal compartment forms. The endosomes from this compartment then begin to migrate centripetally through the cytoplasm in a saltatory way so that within 15 min gold label accumulates in a juxtanuclear endosome compartment. This compartment, which consists mainly of multivesicular bodies, is thus formed by the influx and retention of peripheral endosomal elements and their continued fusion in the juxtanuclear area. Although their overall migration is inward, saltating endosomes frequently reverse their direction of movement. As label builds up in the juxtanuclear area, small vesicles containing gold label continuously pinch off from the larger elements and migrate toward the cell periphery.Experiments with nocodazole and sodium azide show that the saltatory movements, the accumulation and retention of endosomes in the juxtanuclear area, and the separation of vesicles from endosomes are driven by a microtubule-associated, ATP-dependent, motility-generating mechanism.Analysis of the movements shows that although each individual vesicle saltation can occur unpredictably toward the centre or the periphery of the cell, a net centripetal flux is observed. Moreover, it is evident that the probability of migration toward and maintenance in the juxtanuclear area is related to the diameter of the vesicles. We propose a mechanism by which bidirectional saltation along microtubules forming a radial network may be instrumental in the selective concentration of large endosomes in the juxtanuclear area while small vesicles are left free to return to the periphery. This process may be responsible for the sorting of receptors and ligands destined either for intracellular degradation in juxtanuclear lysosomes or, alternatively, for recycling to the plasma membrane.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-675X
    Keywords: Apoptosis ; Bcl-2 ; mitosis ; PC12 ; taxol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Taxol-induced peripheral neuropathy is a commonly-occurring side-effect in the treatment of cancer patients with taxoteres or taxanes. Taxol is known to induce apoptosis in a number of tumor cells. This report documents that, similar to proliferating cells, taxol induces apoptosis in NGF-differentiated PC12 cells, as assessed by exogenous FITC-annexin-V binding and nuclear fragmentation. It is shown that PC12 cells that stably overexpress Bcl-2 are protected against the toxic effect of taxol, as evidenced by the XTT assay and by a decreased fraction of propididum iodide positive cells in a dye exclusion test. Also the number of annexin-V-positive cells and the number of fragmented nuclei are lower in the Bcl-2 transfected cells. The effect is similar to the protective effect of Bcl-2 against NGF deprivation in differentiated PC12 cells. Although taxol forced both wild-type and Bcl-2-overexpressing cells into a mitotic state, only in Bcl-2-overexpressing cells did this lead to the appearance of metabolically active, multi-nucleated cells. This suggests that Bcl-2 is able to induce an alternative escape pathway, downstream of the G2/M block, in taxol-treated differentiated PC12 cells.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 185 (1989), S. 282-295 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: This contribution describes a new microscopic technique aimed at visualizing colloidal gold particles of 20-40 nm diameter as dynamic markers at the light microscopic level. The technique, called nanovid microscopy, is based on the use of contrast enhancement by video techniques and digital image processing. Two applications on living cells are discussed. The first is the receptor-mediated endocytosis of the transferrin receptor, which for the first time can be followed in real time. A second application documents the motion of cell-surface glycoproteins on PTK2-cells.In addition, nanovid microscopy allows the number of gold particles in immunogold staining to be quantified easily. With this tool, the translocation of specific molecules in living cells becomes one of the most enjoyable activities to observe.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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