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  • 1
    Electronic Resource
    Electronic Resource
    Inhibition of papain by N-ethylmaleimide (1974)
    s.l. : American Chemical Society
    Biochemistry 13 (1974), S. 915-920 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00702a014
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  • 2
    Electronic Resource
    Electronic Resource
    Integration of exogenous DNA into the genome of Azotobacter vinelandii (1989)
    Springer
    Archives of microbiology 152 (1989), S. 437-440 
    Details
    ISSN: 1432-072X
    Keywords: Genetic transformation ; Plasmid ; Recombination ; pBR322 ; Chromosomal integration ; β-Lactamase ; Azotobacter vinelandii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The soil bacterium Azotobacter vinelandii was genetically transformed by chromosomal integration to ampicillin and/or tetracycline resistance using restriction endonuclease-linearized plasmids. Polyacrylamide gel electrophoresis of protein extracts from three independently isolated ampicillin resistant transformants showed the presence of a 28 Kd band which is the approximate size of the ampicillin resistance gene product (i.e., β-lactamase). Moreover, with nitrocefin, a chromogenic cephalosporin, as a substrate, it was shown that all of the ampicillin resistant transformants produced functional β-lactamase. DNA hybridization showed that the chromosomal DNA from transformed cells contained plasmid DNA sequences at discrete sites. Growth experiments indicated that stable A. vinelandii transformants that carry functional integrated DNA were physiologically impaired.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446925
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  • 3
    Electronic Resource
    Electronic Resource
    Modulation of plant ethylene levels by the bacterial enzyme ACC deaminase (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 251 (2005), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Soil microorganisms that produce the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase promote plant growth by sequestering and cleaving plant-produced ACC, and thereby lowering the level of ethylene in the plant. Decreased ethylene levels allows the plant to be more resistant to a wide variety of environmental stresses. Here, the biochemistry of ACC deaminase; the environmental distribution, regulation, evolution and expression of ACC deaminase genes; and information regarding the effect of this enzyme on different plants is documented and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsle.2005.07.030
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  • 4
    Electronic Resource
    Electronic Resource
    Activities of chlorophyllase, phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase in the primary leaves of soybean during senescence and drought (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effects of senescence and drought on the levels and activities of chlorophyllase (EC 3.1.1.14), phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) and ribulose-1,5-bisphosphate carboxylase (Rubisco, EC 4.1.1.39) in the intact primary leaves of soybean (Glycine max L. cv. Jackson) were monitored. Plants were grown either (1) for 2 to 8 weeks and the primary leaves harvested every week or (2) for 2 weeks and the plants subjected to drought stress and compared to control plants that were watered daily. In the senescence experiment, chlorophyllase activity changed in parallel with water content, leaf chlorophyll and total protein per unit dry weight of leaf tissue, with all factors increasing in concert during expansion of the primary leaves in the first 4 to 5 weeks of seedling development. Thereafter, all factors, including chlorophyllase activity, declined reaching markedly reduced values at weeks 7 and 8 when the primary leaves were yellow and ready to abscise. PEPC and Rubisco activities peaked in the third week, i.e. well before full leaf expansion, and then declined. In contrast to its response during senescence, chlorophyllase activity per unit leaf dry weight did not change during drought stress, but the specific activity of the enzyme rose and showed an inverse relationship to total leaf chlorophyll and protein content. Rubisco activity was highly sensitive to drought, with decrements observed in the activity and in levels of the large subunit within 2 days of withholding water and before significant changes in leaf water content were detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb05087.x
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  • 5
    Electronic Resource
    Electronic Resource
    Biological activity and colonization pattern of the bioluminescence-labeled plant growth-promoting bacterium Kluyvera ascorbata SUD165/26 (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 35 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Kluyvera ascorbata SUD165/26 is a spontaneous siderophore-overproducing mutant of K. ascorbata SUD165, which was previously isolated from nickel-contaminated soil and shown to significantly enhance plant growth in soil contaminated with high levels of heavy metals. To develop a better understanding of the functioning of K. ascorbata SUD165/26 in the environment, and to trace its distribution in the rhizosphere, isolates of this bacterium were labeled with either green fluorescent protein or luciferase. When the plant growth-promoting activities of the labeled strains were assayed and compared with the activities of the unlabeled strain, none of the monitored parameters had changed to any significant extent. When the spatial colonization patterns of the labeled bacteria on canola roots were determined after seed application, it was observed that the bacterium was tightly attached to the surface of both roots and seeds, and formed aggregates. The majority of the bacterial population inhabited the upper two thirds of the roots, with no bacteria detected around the root tips.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00797.x
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  • 6
    Electronic Resource
    Electronic Resource
    Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria (2003)
    Oxford, UK : Blackwell Science Ltd
    Physiologia plantarum 118 (2003), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: One of the major mechanisms utilized by plant growth-promoting rhizobacteria (PGPR) to facilitate plant growth and development is the lowering of ethylene levels by deamination of 1-aminocyclopropane-1-carboxylic acid (ACC) the immediate precursor of ethylene in plants. The enzyme catalysing this reaction, ACC deaminase, hydrolyses ACC to α-ketobutyrate and ammonia. Several bacterial strains that can utilize ACC as a sole source of nitrogen have been isolated from rhizosphere soil samples. All of these strains are considered to be PGPR based on the ability to promote canola seedling root elongation under gnotobiotic conditions. The treatment of plant seeds or roots with these bacteria reduces the amount of ACC in plants, thereby lowering the concentration of ethylene. Here, a rapid procedure for the isolation of ACC deaminase-containing bacteria, a root elongation assay for evaluating the effects of selected bacteria on root growth, and a method of assessing bacterial ACC deaminase activity are described in detail. This should allow researchers to readily isolate new PGPR strains adapted to specific environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2003.00086.x
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  • 7
    Electronic Resource
    Electronic Resource
    Root-surface colonization of black mangrove seedlings by Azospirillum halopraeferens and Azospirillum brasilense in seawater (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 29 (1999), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Inoculation of axenic black mangrove seedlings in seawater for 8 days with either the terrestrial halotolerant plant growth-promoting bacterium Azospirillum halopraeferens or with Azospirillum brasilense produced heavy colonization of the root surface. The colonization pattern was different for the two strains. A. halopraeferens yielded mainly single cells embedded in a thick sheath, whereas A. brasilense produced primarily microaggregates. A. brasilense cells were anchored to the root surfaces and to themselves by a network of fibrillar material. Both bacterial strains survived in seawater (approximately 104 colony forming units per ml) for more than 30 days, for 70 days in saline water (A. brasilense) and colonized mangrove roots at a very high population density. A. halopraeferens was a better root surface colonizer, whereas the A. brasilense population was greater in the entire root. This work is the initial stage of studies designed to assess the feasibility of using terrestrial plant growth-promoting bacteria for the inoculation of marine plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1999.tb00619.x
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  • 8
    Electronic Resource
    Electronic Resource
    Synergism between Phyllobacterium sp. (N2-fixer) and Bacillus licheniformis (P-solubilizer), both from a semiarid mangrove rhizosphere (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 35 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Mangrove seedlings were treated with a mixture of two bacterial species, the slow-growing, N2-fixing bacterium Phyllobacterium sp. and the fast-growing, phosphate-solubilizing bacterium Bacillus licheniformis, both isolated from the rhizosphere from black, white, and red mangroves of a semiarid zone. Nitrogen fixation and phosphate solubilization increased when the mixture was used compared to the effects observed when adding individual cultures, notwithstanding that there was no increase in bacterial multiplication under these conditions. Inoculation of black mangrove seedlings in artificial seawater showed the mixture performed somewhat better than inoculation of the individual bacterium; more leaves were developed and higher levels of 15N were incorporated into the leaves, although the total nitrogen level decreased. This study demonstrates that interactions between individual components of the rhizosphere of mangroves should be considered when evaluating these bacteria as plant growth promoters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00802.x
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  • 9
    Electronic Resource
    Electronic Resource
    Factors affecting the expression of foreign proteins inEscherichia coli (1987)
    Springer
    Journal of industrial microbiology and biotechnology 1 (1987), S. 277-282 
    Details
    ISSN: 1476-5535
    Keywords: Recombinant DNA ; Gene expression ; Genetic engineering ; Biotechnology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A variety of factors affect the expression of foreign proteins inEscherichia coli. These include: promoter strength, efficiency of ribosome binding, stability of the foreign protein inE. coli, location of the foreign protein inE. coli, the codons used to encode the foreign protein, the metabolic state of the cell, and the location, stability and copy number of the foreign gene. This paper contains a critical review of these factors with the idea that a detailed understanding of them is the key to the development of strategies for the efficient large-scale production of foreign proteins inE. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569305
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  • 10
    Electronic Resource
    Electronic Resource
    Teamwork in phytoremediation (2004)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 526-527 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The high cost and technical difficulty of removing many noxious and toxic organic compounds (xenobiotics) from the environment has provided impetus to studies in which plants are used as an inexpensive means of environmental cleanup (phytoremediation). In this issue, Barac et al. describe a new ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0504-526
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