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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 807 (1985), S. 216-219 
    ISSN: 0005-2728
    Keywords: (Spinach chloroplast) ; Cytochrome b"6f complex ; Inhibitor ; Photosystem II ; Stigmatellin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0005-2728
    Keywords: Chlorophyll a fluorescence ; EPR ; Electron transport ; Photoinhibition ; Photosystem I ; Photosystem II
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 1187 (1994), S. 335-346 
    ISSN: 0005-2728
    Keywords: Intact chloroplast ; LHC II ; Phosphorylation ; Photosystem II ; Protein kinase ; Protein phosphatase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 86 (1992), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Long term fumigation of 4-year-old spruce trees with ozone concentrations up to 200 nl l−1 has only minor effects on the photosynthetic activities measured as chlorophyll a fluorescence. Nevertheless, it drastically changes the turnover of the D-1 reaction center polypeptide of photosystem II. During summer, fumigation with ozone for 2 weeks resulted in an almost 4-fold stimulation of the light dependent incorporation of [14C] leucine into the D-1 protein in the exposed trees. The amount of immunodetectable D-1 protein remained constant when based on chlorophyll. This indicates that exposure to ozone stimulates both the synthesis and the degradation of the D-1 protein. When spruce trees were exposed during winter for 4 weeks to 100 and 200 nl l−1 ozone, respectively, an almost 3-fold increase of the amount of immunodetectable D-1 protein per chlorophyll in the exposed trees was observed. This can be explained by a varying stimulation of D-1 protein synthesis and degradation depending on the different physiological conditions. Since so far the D-1 protein has been found only as a component of photosystem II reaction centers, one has to assume that the relative content of photosystem II reaction centers also increases under certain stress conditions. The increased turnover of the D-1 protein in trees exposed to ozone explains the synergistic effects of stress conditions and high light intensities often observed in the field.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 85 (1992), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Long-term fumigation with different mixtures of air pollutants, such as O3, NO2 and SO2, in combination with potassium deficiency in 4-year-old spruce trees shows clearly that the photosynthetic apparatus reacts very sensitively to environmental stress. Even when no change in photosynthetic oxygen evolution of the leaves was measurable, the content of D-1 protein in the reaction center of photosystem II already increased significantly at low stress conditions. With increasing concentrations of pollutants, the content of D-1 protein decreased considerably. This pattern was observed with all combinations of gases, although they differed in effectiveness. The most potent effect on the content of D-1 protein was found with a combined fumigation of SO2 plus NO2. Obviously, the ratio between synthesis and degradation of the D-1 protein is influenced by stress factors. However, the content of D-1 protein was not strongly correlated with photosynthetic oxygen evolution in whole needles nor with electron transport rate in isolated thylakoids. Under controlled potassium deficiency, effects similar to those observed upon ozone fumigation developed. The results suggest unspecific stress answers for the D-1 content and the photosynthetic reactions rather than specific responses.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2285
    Keywords: Element content ; Element distribution ; Forest decline ; Micro-PIXE ; Picea abies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Proton-induced X-ray microanalysis (micro-PIXE) permits the simultaneous determination of the content and the distribution of elements with atomic numbers higher than Z = 13 in biological samples. This method was used to investigate element content and localization in cross-sections of 6-month-old spruce needles. It was possible to detect the elements silicon, phosphorus, sulphur, chlorine, potassium, calcium, manganese, iron and zinc in semithin (10 μm) sections of the needles. The localization of the cationic elements like potassium, calcium and manganese was determined in the one-dimensional line scan mode and in the two-dimensional raster scan mode. To demonstrate the usefulness of this method for forest decline research, element content and localization were compared in needles from two trees, which differed in their degree of damage. We were able to detect differences in the amount of cations and in their distribution inside the needles.
    Type of Medium: Electronic Resource
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