ISSN:
1432-2013
Keywords:
Renal tight cultured cell line (A6)
;
Specific membrane conductances
;
Barium
;
Microelectrodes
;
Circuit analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Confluent monolayers of the cultured renal distal tubule cell line (A6) were impaled with microelectrodes under short-circuit conditions. Specific membrane conductances were calculated from equivalent circuit equations. Transport properties of the apical and basolateral membranes were investigated during control conditions and short-term increases in basolateral potassium concentration [K+] from 2.5 to 20 mmol/l, with or without 0.5 mmol/l Ba2+ at the basolateral side. As in most other epithelia, the apical membrane represents the major resistive barrier. Transcellular, apical and basolateral membrane conductances (g c, g o and g i respectively), obtained from 22 acceptable microelectrode studies, averaged 61, 80 and 292 μS/cm2, respectively. There was a highly significant correlation between short-circuit current (I sc) and g o, whereas g i was unrelated to I sc. The I sc, which averaged 4.1 μA/cm2, was almost completely blocked by amiloride. This was associated with fast hyperpolarization; the intracellular potential (V sc) increased from −69 to −83 mV and the fractional apical resistance rose to nearly 100%. Using the values of V sc during amiloride at normal and high [K+], an apparent transference number for K+ at the basolateral membrane of 0.72 can be calculated. This value corresponds with the decrease in g i to about 25% of the control values after blocking the K+ channels with Ba2+. The nature of the remaining conductance is presently unclear. The cellular current decreased during high [K+] and Ba2+, in part resulting from reduction of the electrochemical gradient for apical Na+ uptake due to the depolarization. In addition, g o decreased to less than 40%, which is considerably lower than predicted by the constant-field equation; this might indicate voltage sensitivity of the apical Na+ permeability.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00370940
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