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Notes: Seven cultivars of white clover (Trifolium repens L.) (Kent, S184, Huia, Menna, Donna, Alice and Nesta) and a commercial mixture, ‘Ensign’, were strip-seeded into an upland perennial rye-grass (Lolium perenne L.) sward in late June 1986. Swards were first grazed by sheep, either on 5/6 August (early) or on 19/20 August (late) and then every 14–21 days (frequently) or 28–42 days (infrequently) during 1986, followed by a common grazing regime in 1987. During April to mid-June 1988 the swards received either a moderate amount of nitrogen or none and were cut frequently or once only in mid-June. Growth of individual seedlings was assessed before and after grazing during 1986 and stolon accumulation and distribution and sward colonization were assessed during 1987 and 1988.All cultivars emerged rapidly and satisfactorily and there were no consistent significant differences in the overall dry matter accumulation per seedling during establishment. During the first autumn the proportion of the aboveground biomass removed during grazing was smallest in Kent (c. 20%) and largest in Nesta (c. 40%). Kent and S184 produced most leaves and stolons and the greatest length of stolons per seedling and per individual stolon, and Nesta and Alice the fewest leaves and stolons and shortest stolons. Seedlings grazed early had heavier and longer stolons than those grazed late; those grazed frequently had more leaves, stolons and growing points than those grazed infrequently, especially following early grazing.During 1987 Kent and S184 had consistently the largest number of stolon growing points, and weight and length of stolons per unit area; these two cultivars and Nesta also colonized the sward more rapidly than the other cultivars. All cultivars contributed substantially and similarly to herbage production in late September. There were no residual effects of the 1986 treatments after the summer of 1987.During 1988 additions of nitrogen fertilizer at 100 kg N ha-1 or allowing the herbage to remain undefoliated between mid-April and mid-June both independently halved the number of stolon growing points per unit area; together they reduced it by 80%. Nitrogen also, on average, halved stolon weights but less so in Nesta, Alice and Huia and more so in all other cultivars. Infrequent defoliation greatly decreased stolon weights in Kent and S184 but had no significant effects on the other cultivars. Sward colonization was almost complete by June and entirely so by October for all cultivars in all treatments.Implications of the results for the after-management of strip-seeded white clover are discussed.

Notes: Small plots of a Festuca-Agrostis upland sward on a peaty gley podsol were strip-seeded during late June 1986 with white clover cvs Aberystwyth S184 or Menna at 4 kg ha−1 and defoliated early (20 August) or late (3 September) and then frequently or infrequently (every 2 weeks or 4 weeks) until the end of September. All plots were defoliated in early November, at 3-weekly intervals during the growing season in 1987 and then grazed rotationally during 1988.Satisfactory seedling establishment, representing 46% emergence, was achieved 5 weeks after sowing. The differential defoliation regimes had no persistent significant effects on clover development. S184 soon produced more leaves per seedling than Menna and a smaller proportion of its leaf number and weight were removed at each defoliation. Following large losses of leaves over the 1986–87 winter, SI84 had significantly more leaves per stolon than Menna; subsequently it also colonized the sward at a quicker rate. During 1987 amounts of herbage harvested (6.1 t ha−1) were similar with the two clover cultivars, with S184 contributing 47% and Menna 44% of this respectively. SI84 made a larger contribution to yield during May and June but Menna was more productive during September and October. During 1988 clover populations were maintained with rotational grazing without additional fertilizer inputs.The results show that, despite initial soil and climatic contraints, both small and medium-leaved clovers can be strip-seeded into upland swards with large subsequent benefits to yield and herbage quality. However, they also indicate the need for further experiments to determine the influence of sward morphology and defoliation regime on stolon branching rates and accumulation of growing points which, in turn, govern sward colonization.

Notes: The effects of different defoliation regimes on the growth and development of three contrasting white clover cultivars (S184, Menna and Alice) were assessed in three experiments in the glasshouse. Experiment 1, with clover growing on its own, investigated the effects of three times of onset × two intensities of defoliation. In Experiment 2, clover was grown with grass and there were two times of onset × two heights × two frequencies of defoliation. Experiment 3, also with grass, investigated the effects of changing defoliation frequency at different intervals from sowing.All clover cultivars responded similarly to the various treatments and there were no interactions between time of onset and subsequent defoliation regimes in Experiments 1 and 2. Without competition from grass (Experiment 1), defoliating early at the three leaf-stage of clover decreased the number of growing points by 32% and stolon weights and lengths by 50% compared with delaying defoliation until the nine leaf-stage. Maintaining one compared with two leaves per growing point had similar effects. Over 17 weeks undefoliated seedlings produced ten times more stolon than early defoliated or intensively defoliated seedlings. In competition with grass (Experiment 2) delaying defoliation significantly decreased all aspects of stolon growth. Seedlings growing in swards defoliated frequently and closely had most growing points whereas those defoliated infrequently had least. Stolon lengths and weights were larger for seedlings growing in swards defoliated frequently than for those defoliated infrequently at both heights of cutting. Mean weight of stolon per unit length was greater when swards were defoliated at 6 cm than at 2 cm height. Changing defoliation from every 2 weeks to every week (Experiment 3) decreased stolon growth slightly when the change was made early but increased it when the change was made late, although similar amounts of stolon were produced by seedlings continuously defoliated throughout every week and every 2 weeks.The results are discussed in relation to the seedlings' leaf complements and growth habit; the over-riding influence of grass competition is highlighted. The possibility of devising optimal defoliation strategies and the need to test these in the field are also outlined.

Notes: Seeds of white clover (Trifolium repens) were sown alone or in fluid, or pre-germinated in fluid on to the soil surface of slots cut in an old Agrostis/Festuca sward. Seeds were left uncovered or covered with a thin layer of soil and irrigated from the outset or after 14 days delay.With irrigation from the outset, seedlings established soonest from pre-germinated seeds but final percentage establishment ranged only from 50 to 63% for the three states of seed. With delayed Irrigation, however, few pre-germinated s1 survived and although germination of dry seed was delayed, final percentage establishment was unaffected. Soil covering increased establishment slightly. The number of leaves per seedling was greatest with pre-germination and early irrigation and least with pre-germination and delayed irrigation. Delayed irrigation also decreased the number of leaves per seedling for seedlings grown from dry seed.The effects of early irrigation persisted so that at 84 days it significantly increased total yield of clover from all three states of seed, on average threefold, but much more from pre-germinated than from dry seed. Soil covering greatly increased yield, especially where irrigation had been applied from the outset. The interaction of early irrigation and soil covering was even more pronounced for stolon weights, and stolon growth of Individual seedlings appeared to benefit more from soil covering than from early irrigation.The results showed the importance of early seedling emergence, but no advantages of fluid sowing per se. They also indicated large overall beneficial effects of early irrigation and its additive interaction with soil covering.

Notes: Seedlings of white clover (cv. Grasslands Huia) were introduced as spaced plants into 3-year-old monoculture plots of eight grass species (Agrostis capillaris, Agrostis stolonifera, Dactylis glomerata, Festuca rubra, Holcus lanatus, Lolium perenne, Phleum pratense and Poa pratensis) during June 1984. In mid-April 1985 plots were split for application of propyzamide at the following concentrations: 0, 0·2, 0·4, 0·6 kg a.i. ha-1.During 1984 differences between clover seedling growth in the different grass species became apparent within 2 weeks; growth was greatest in F. rubra, P. pratensis and H. lanatus and smallest in D. glomerata, L. perenne and P. pratense. During 1985, when more N fertilizer was given, H. lanatus and D. glomerata, were equally competitive and clover contributed only 16–18% of the total herbage yield of 10·4 t ha-1 in them, compared with 33–50% of yields ranging from 9·6 to 119 t ha-1 in the other six species. Propyzamide decreased grass growth in mid-season by more than 50% but there was little overall persistent benefit to clover yield, except for A. stolonifera and P. pratense with 0·4 and 0·6 kg ha-1 treatments. Dactylis glomerata and perenne were least and H. lanatus, F. rubra and P. pratensis most suppressed by the herbicide.Possible reasons for the overall large clover contribution from a very sparse seedling population and the relatively small effects of propyzamide are discussed as well as future work required to improve the predictability of effects of grass suppression.

Notes: White clover (cv. Grasslands Huia) was slot-seeded in April 1982 into plots of permanent pasture of differing botanical composition and reserves of soil phosphate. In one investigation the short-term effects of placing different amounts of phosphate (P2O5) in different positions in relation to the slot were investigated. In another, emphasis was given to effects of a grass-suppressing herbicide, propyzamide, on herbage growth and clover contributon during the second year.Fewer seedlings established on plots with a high initial P status and abundant in Holcus lanatus and Alopecurus pratensis than on plots deficient in P2O5 and dominated by Agrostis capillaris and Festuca rubra. P2O5decreased numbers of establishing clover seedlings when applied in the slots but not when placed beneath them. Growth was increased most when placement was beneath the slot. Application of P2O5to plots previously supplied with it resulted in 7.2 g total above ground and 1.7 g stolon dry weight (m row)−1compared to 1.3 and 0.1 g respectively in plots with low reserves and DO applied P2O5.Propyzamide greatly increased the yield and the contribution of clover in the presence of high initial P status and applied P2O5. Without propyzamide, clover made only a small contribution until July, averaging 7% for the whole season with little benefit from residual P. Clover growth in late summer was increased from 41 to 292 kg DM ha−1 where P2O5 was applied to P-deficient plots sprayed with propyzamide in the previous autumn. The large increases in clover contribution following propyzamide application were accompanied by small decreases in total seasonal yield of grass. The proportions of H. lanatus and. F. rubra. were decreased and of A. capillaris, increased.Spread of clover from the slots was slow during the first year but was encouraged by both P2O5 and propyzamide during the second year. Factors requiring further investigation are discussed.

Notes: The effects of delayed emergence of white clover (cv. Grasslands Huia) seedlings, following slot-seeding during spring and early summer 1983, were simulated by sowing seeds at intervals into slots cut in turves of permanent grassland in soil-filled tanks or in the field. The resulting spread and growth of the species were assessed during the following 15–18 months. The effects of propyzamide, a grass-suppressing herbicide (at 0·2 kg ha-1), were also investigated in the field.A delay of 10 or 18 days in the sowing of seeds in turves in the tanks reduced clover dry weight yield from 23 to 11 during 1983 and from 118 to 96 g (0·12 m)-2 during 1984. Spread of stolons from the slots was also greatly delayed and effects persisted for at least a year. Increased clover growth on the earliest sown treatment more than compensated for a slightly smaller yield of grass.In the field, yields of clover from late summer 1983 until spring 1984 closely reflected the order of emergence. Afterwards, differences were less clear-cut but the cumulative yield of clover until August 1984 of the earliest-sown treatment was twice that of a treatment sown 20 d later. Effects of delayed sowing in summer 1983 were more pronounced on stolons than on foliage growth when measured in September 1984, with significant reductions in many stolon attributes even with 8 d delay in sowing. Application of propyzamide in autumn 1983 increased clover yield during 1984 from 1·7 to 3·0 and total herbage yield from 7·1 to 8·6 t ha-1.The results demonstrated some of the benefits of rapid seedling emergence and of the use of a grass-suppressing herbicide, but did not indicate any interaction between the two factors.