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  • 1
    Electronic Resource
    Electronic Resource
    Vasoactive intestinal polypeptide (VIP) containing cells in the developing rat occipital hemisphere (1990)
    Springer
    Anatomy and embryology 182 (1990), S. 69-78 
    Details
    ISSN: 1432-0568
    Keywords: Vasoactive intestinal polypeptide ; Forebrain ; Ontogeny ; Subventricular zone ; Glia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Numerous cells were observed to show intense vasoactive intestinal polypeptide (VIP) immunoreactivity from birth to postnatal day 8 in the subventricular zone of the rat occipital hemisphere. This cell population was markedly reduced by postnatal day 8, but isolated clusters of VIP cells persisted into adulthood. In addition, long, L-shaped VIP fibers were seen in the hemispheric wall up to postnatal day 16, but not in the adult. Parallel to the reduction in number of the subventricular VIP cells an increasing number of VIP cells appeared in the neo- and allocortex, developing by postnatal day 12 all the features of the mature cortical bipolar fusiform neurons. As possible alternatives, the migration of subventricular VIP cells into the cortex, the transient character of the subventricular VIP population or the expression of VIP by radial glia are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00187528
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  • 2
    Electronic Resource
    Electronic Resource
    Radial glia in the developing mouse cerebral cortex and hippocampus (1981)
    Springer
    Anatomy and embryology 163 (1981), S. 331-343 
    Details
    ISSN: 1432-0568
    Keywords: Cerebral cortex ; Development ; Glial fibrillary acidic protein ; Hippocampus ; Immunocytochemistry ; Radial glia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The regional distribution of radial glia in the developing cerebral cortex and the hippocampus of the mouse was studied using silver impregnation and immunocytochemical staining for glial fibrillary acidic protein (GFAP). Whilst the former technique revealed radial fibres at a slightly earlier age, immunocytochemistry gave a better picture of their general distribution and enabled systematic study of the appearance and disappearance of GFAP-positive radial glia throughout the cortex. Although a clear association between migrating neurones and radial glia was evident in the later stages of cortical plate formation, this relationship was not apparent in all cortical regions nor at the very early stages of the formation of the cortical plate. Even after allowing for a delayed appearance of GFAP immunoreactivity in relatively mature radial glia, the uneven distribution of these cells, their appearance after the cortical plate has already been formed, and their regional development in a pattern dissynchronous with that of the cortical plate argue against a general role of these structures in neuronal migration in the mouse, although there are notable phylogenetic differences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315709
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  • 3
    Electronic Resource
    Electronic Resource
    Long-term effects of anterograde degeneration on astroglial reaction in the rat geniculo-cortical system as revealed by computerized image analysis (1993)
    Springer
    Anatomy and embryology 187 (1993), S. 1-7 
    Details
    ISSN: 1432-0568
    Keywords: Glial fibrillary acidic protein ; Astrocyte ; Visual cortex ; Anterograde degeneration ; Image analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Changes in the expression of glial fibrillary acidic protein by astrocytes in the primary visual cortex of adult albino rats were analyzed with immunohisto-chemistry after unilateral destruction of the dorsal lateral geniculate nucleus. An increase in number of glial fibrillary acidic protein-immunoreactive astrocytes could be detected in the visual cortex of the side ipsilateral to the lesion in the short-term survival group (7–11 days post lesion), but this increase was extremely reduced after a postlesional survival time of 150 days. The quantitation of the glial response by image analysis showed, that the initial increase was mainly localized in the cortical layers II–IV, where the geniculo-cortical input terminates. The transient nature of this process was revealed by the measurements in the long-term survival group, where differences between experimental and control sides were substantially reduced. We conclude, that the remote glial response in the visual cortex is transient and that its disappearance indicates the end of a postlesional adaptation period in the neuropil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00208191
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  • 4
    Electronic Resource
    Electronic Resource
    Immunocytochemical demonstration of glial fibrillary acidic protein in mouse tanycytes (1981)
    Springer
    Anatomy and embryology 162 (1981), S. 217-222 
    Details
    ISSN: 1432-0568
    Keywords: Glial cells ; Glial fibrillary acid protein ; Tanycytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immunohistochemical techniques were used to stain for the astrocytespecific glial fibrillary acidic protein (GFAP) in the cells lining the third ventricle of the developing and mature mouse brain. Before birth immunoreactive tanycytes were only observed in the infundibular recess of the median eminence, where they could first be seen at embryonic day 17. They possessed long processes running towards the ventral surface of the brain. During the early postnatal period GFAP-positive tanycytes gradually appeared throughout the third ventricle, although the ependymal cells themselves remained unstained. The tanycytes retained their immunoreactivity for anti-GFAP serum in the adult, and were also evident in the adult rat third ventricle. It is suggested that the presence of GFAP in these specialised cells of the third ventricle indicates that they, the transient radial glia of the developing cerebral cortex, the persistent Bergmann glia of the cerebellum, similar astrocytes with radial processes in the hippocampal dentate gyrus and conventional astroglia are all closely related cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306493
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  • 5
    Electronic Resource
    Electronic Resource
    The morphology of microtubules in incubated synaptosomes. Effect of low temperature and vinblastine (1979)
    Springer
    Experimental brain research 35 (1979), S. 387-393 
    Details
    ISSN: 1432-1106
    Keywords: Synaptosome ; Microtubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A time-dependent rise in the percentage of microtubule-containing synaptosomes was observed reaching a 41–47% peak after 30 min incubation at room temperature (22–25 ° C) in a saline medium. Fixation at low temperature or incubation with vinblastine decreased markedly the number of synaptosomes containing microtubules. It was concluded that incubation with cations and processing at room temperature were prerequisites of optimal preservation of synaptosomal microtubules. Their spatial orientation relative to the synaptic cleft was deduced from statistical examination of profiles in different orientations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236622
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Electron histochemical observation of succinic dehydrogenase activity in various parts of neurons (1969)
    Springer
    Experimental brain research 8 (1969), S. 66-78 
    Details
    ISSN: 1432-1106
    Keywords: Succinic dehydrogenase ; Mitochondria ; Neurons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Distribution of succinic dehydrogenase (SDH) activity in various parts of neurons, perikarya, dendrites, axons and axon terminals was studied at the electron microscope (EM) level by means of a copper-ferrocyanide method recently developed by xxKerpel-Fromus and xxHajos (1967). Mitochondria of the perikarya, both in the CNS and in ganglia, show a marked SDH activity, which is still strong in proximal parts of the dendrites, but which greatly decreases in more distal parts of the dendrites. SDH activity becomes hardly detectable in the axons and appears consequently lacking in the synaptic axon terminals. It is assumed, however, that this is due to a level of activity that is below the sensitivity of the method employed. Cytochrome oxidase activity has been shown in earlier studies by the same authors not to be different in mitochondria of perikarya and axon terminals. The possible significance of this striking difference in somal and synaptic mitochondria of neurons is briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234926
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  • 7
    Electronic Resource
    Electronic Resource
    The effect of pentobarbital, chloralhydrate, ether and protoveratrine on the distribution of synaptic vesicles in rat cortical synaptosomes (1978)
    Springer
    Experimental brain research 33 (1978), S. 91-99 
    Details
    ISSN: 1432-1106
    Keywords: Synaptosome ; Anaesthetics ; Vesicle hypothesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rat cortical synaptosomes were incubated in a saline medium. On the addition of pentobarbital, chloralhydrate, ether and protoveratrine a redistribution of synaptic vesicles was revealed by electron microscopy. The anaesthetics decreased the number of synaptic vesicles attached to the presynaptic membrane while protoveratrine increased them. It is assumed that these agents affect the mechanism of vesicle reformation from the synaptosomal membrane and/or vesicle migration towards the synaptic cleft.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238797
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  • 8
    Electronic Resource
    Electronic Resource
    A rapid procedure for obtaining a preparation of large fragments of the cerebellar glomeruli in high purity (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 24 (1975), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1975.tb03913.x
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  • 9
    Electronic Resource
    Electronic Resource
    A method for the electron microscopic demonstration of cytochrome oxidase in fresh and formalin-prefixed tissues (1967)
    Springer
    Histochemistry and cell biology 10 (1967), S. 216-223 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Burstone's reaction is adapted for electron microscope purposes. Sections of 300–500 μ thickness cut by free hand are incubated for sixty minutes in N-phenyl-p-phenylenediamine at room temperature. No coupling agent is used. The dye polymere gained is postchelated with 0.5% copper sulphate dissolved in 4% buffered formalin solution. After thorough washing a second postchelation is performed with potassium ferrocyanide. Double postchelation reduces the otherwise high lipid solubility of the reaction product, so that it withstands embedding into epoxy resins. Postfixation with osmic acid adds further contrast to the reaction product. Cell structure — even in central nervous tissue — could be preserved satisfactorily without major loss of enzyme activity by the use of a brief formalin perfusion prior to incubation. The reaction product appears in the form of droplets localized in the mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304868
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  • 10
    Electronic Resource
    Electronic Resource
    Localization of α-tubulin immunoreactivity to cerebellar Bergmann glia with the TU 01 antibody (1984)
    Springer
    Histochemistry and cell biology 81 (1984), S. 297-299 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The TU 01 antitubulin antibody specific for α-tubulin was demonstrated, by light-and electron-microscopic immunocytochemistry, to stain selectively the Bergmann glia of rat cerebellum. Within the glial cells and their fibres the microtubules, the smooth endoplasmic reticulum, the outer mitochondrial membranes and the ribosomal rosettes of the cell body were decorated by the antibody. The high selectivity of this antibody is discussed in terms of tubulin microheterogeneity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495643
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