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The Interaction between Beta 2-Microglobulin (ßm) and Purified Class-I Major Histocompatibility (MHC) Antigen (1994)

PEDERSEN, L. Ø. ; HANSEN, A. S. ; OLSEN, A. C. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Scandinavian journal of immunology 39 (1994), S. 0

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ISSN: 1365-3083

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: The function of MHC class-I molecules is to sample peptides from the intracellular environment and present them to CD8+ cytotoxic T lymphocytes. To understand the molecular details of the assembly (and disassembly) of peptide-ß2m-class-I complexes a biochemical peptidc-class-I binding assay has been generated recently and this paper reports on a similar assay for the interaction between ß2m and class I. As a model system human ß2m binding to mouse class I was used. The assay is strictly biochemical using purified reagents which interact in solution and complex formation is determined by size separation. It is specific and highly sensitive. The observed affinity of the interaction, KD, is close to 0.4 nw. The rate of association at 37 C is very fasi (the ka is around 5 × 104/M/s) whereas the dissociation is slow (the kd is around 8 × 10−6/s); the ratio of dissociation to association yields a calculated KD close to the observed value. At 37° C almost all of the purified class I participates in binding of the exogenously offered ß2m showing that a considerable exchange of the endogenous ß2m occurs. Finally, it was demonstrated that exogenous ß2m enhances binding to MHC class-I of short perfectly-matching peplides as well as longer peptides.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-3083.1994.tb03341.x

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Identification of mustard related compounds in aqueous samples by gas chromatography/mass spectrometry (1989)

D'Agostino, P. A. ; Provost, L. R. ; Hansen, A. S. ; [et al.]

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 18 (1989), S. 484-491

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ISSN: 0887-6134

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: Capillary column gas chromatography/mass spectrometry, under electron impact and chemical ionization conditions, was used for the detection and identification of mustard and other sulfur vesicant-related decomposition products in a number of aqueous sample extracts. Interpretation of the mass spectral, infrared, chromatographic and trimethylsilyl derivatization data acquired during this study enabled the identification of nineteen sulfur vesicant-related hydrolysis products. Many of the compounds characterized during these analyses, including a number of ether/thioether macrocycles and vinyl alcohols, have either not been previously reported or have not been previously associated with the decomposition of munitions-grade mustard.

Additional Material: 6 Ill.