Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Stereochemistry of the isoprenylation of tryptophan catalyzed by 4-(.gamma.,.gamma.-dimethylallyl)tryptophan synthase from Claviceps, the first pathway-specific enzyme in ergot alkaloid biosynthesis (1990)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 112 (1990), S. 297-304 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00157a046
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Systemic effects of insulin-like growth factor-II produced and released from Wilms tumour tissue (1993)
    Springer
    European journal of pediatrics 152 (1993), S. 102-106 
    Details
    ISSN: 1432-1076
    Keywords: IGF-II ; Hepatoblastoma ; Nephroblastoma ; Tumour hypoglycaemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The concentration of mRNA of insulin-like growth factor-II is (IGF-II) much elevated in some embryonic tumours such as Wilms tumour (nephroblastoma). In order to prove whether or not IGF-II is produced by the tumour tisue, IGF-II was extracted from freshly frozen tissue of Wilms tumour and hepatoblastoma. Normal adjacent tissue of kidney and liver was used as a control. The total IGF-II in Wilms tumour was 548.4±77.4 ng/g (n=7) compared to 112.8±38.2 ng/g (n=5) in kidney. In two hepatoblastomas, it was 96.1±22.8 ng/g compared to 30.1±14,2ng/g in normal liver. Small pieces of fresh primary tissue of several Wilms tumours were successfully transplanted into immunodeficient nude mice. In serum of tumour-bearing mice IGF-II was elevated compared to normal mice. Liver weight of tumour bearing mice was higher than that of control mice (2.29±0.4g and 2.02±0.06 g;P〈0.005). This was also found for kidney weight (0.58±0.01 g vs. 0.51±0.01 g in controls,P〈0.001). In contrast, serum glucose (9.73±0.29 mmol/l compared to 11.80±0.42 mmol/l in controls,P〈0.0005) was decreased. However, there was no significant difference in nose-tail length of tumour-bearing compared to control mice. These results demonstrate that besides the highly increased IGF-II-mRNA, the synthesis of the peptide IGF-II and its release into circulation are also elevated in Wilms tumour transplanted into nude mice. Elevated circulating IGF-II is likely to stimulate growth of some inner organs and has a glucose lowering effect but does no stimulate skeletal growth. This is further evidence for the importance of IGF-II as a growth factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02072483
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Three cases of neonatal herpes simplex virus infection presenting as fulminant hepatitis (1990)
    Springer
    European journal of pediatrics 149 (1990), S. 555-559 
    Details
    ISSN: 1432-1076
    Keywords: Neonate ; Hepatitis ; Herpes simplex ; Acyclovir
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report three cases of neonatal herpes simplex virus (HSV) infection presenting as fulminant hepatitis. None of the patients had clear risk factors for HSV infection and they all died. Antiviral treatment for HSV is currently available but must be administered early in the course of the disease before irreversible liver tissue damage is present. Since the diagnosis may be difficult to establish, we wish to draw the attention of clinicians to the presentation of neonatal HSV infection and suggest that in such cases viral cultures, including culture of liver tissue, should be obtained early and antiviral treatment administered while awaiting the culture results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01957691
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Occupational UVC-induced exacerbation of atopic dermatitis in a welder (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Contact dermatitis 35 (1996), S. 0 
    Details
    ISSN: 1600-0536
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0536.1996.tb02343.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    The enthalpy of formation of the CdGaZn liquid system
    Amsterdam : Elsevier
    Thermochimica Acta 217 (1993), S. 19-28 
    Details
    ISSN: 0040-6031
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0040-6031(93)85094-P
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    A realistic approach to the sensitivity of PCR-DGGE and its application as a sensitive tool for the detection of clonality in cutaneous T-cell proliferations (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Experimental dermatology 6 (1997), S. 0 
    Details
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract The practical value of the detection of clonality within the T-cell receptor gamma locus by polymerase chain reaction for the diagnosis of cutaneous T-cell lymphomas is well known. However, studies dealing with this subject so far, with special emphasis on the sensitivity of the technique in comparison to, for example, Southern blotting have used mixtures of DNA in various concentrations instead of using mixtures of the cells involved, which would reflect the in vivo situation in a more realistic scope. The purpose of this study was therefore to determine the sensitivity and the limitations of the PCR assay by dilution experiments, using mixtures of cells. Furthermore we studied its applicability to cutaneous T-cell proliferative disorders. Two clonal T-cell lines (MyLa and Jurkat) served as positive control. Dilutions of MyLa cells, cultured normal human keratinocytes and peripheral blood mononuclear cells from lymphoma negative volunteers were used to assess the sensitivity of the PCR-DGGE assay. Skin samples from 4 patients with cutaneous T-cell lymphoma, 1 lesional lymph node, 2 blood samples from a patient with Sézary syndrome and 4 lymphoma-negative tissue samples were analysed. Two samples were uncertain for diagnosis of lymphoma. The PCR-DGGE assay consisted of a 2-round nested PCR with consensus primers within the TCR-gamma locus followed by electrophoretic separation of the product along a denaturing urea/formamide gradient gel. PCR-DGGE sensitivity was, to our knowledge, for the first time investigated for mixtures of lymphocytes (clonal and polyclonal) and keratinocytes. Clonal T-cells were detected in a concentration between 1–0.1% in keratinocytes, whereas the sensitivity was generally lower upon dilution in peripheral blood mononuclear cells or in a mixture of keratinocytes and peripheral blood mononuclear cells. Nevertheless, T-cell clonality was detected in 2 blood samples of a patient with Sézary syndrome, which were negative by Southern blot analysis. The crucial point of this work was the new approach to establish the sensitivity of the PCR-DGGE, in a way which more closely mimics the condition of clinical specimens. Instead of mixing and amplifying DNA extracted from clonal T-cell lines and polyclonal bone marrow cells, we amplified DNA from clonal and polyclonal cells which had been mixed in various ratios before DNA extraction. Polymerase chain reaction in conjunction with denaturing gradient gel electrophoresis is a sensitive and versatile molecular tool for the assessment of clonality of suspect cutaneous lesions. The determination of sensitivity using DNA extracted from premixed cells more closely corresponds to the actual test situation when testing skin samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0625.1997.tb00158.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Nachweis des intermediären Trophoblasten als Beleg einer intrauterinen Schwangerschaft (1994)
    Springer
    Der Pathologe 15 (1994), S. 28-31 
    Details
    ISSN: 1432-1963
    Keywords: Schlüsselwörter: Intermediärer Trophoblast – Zytokeratine – Abort – Extrauteringravidität – Y-Chromosom – In-situ-Hybridisierung ; Key words: Intermediate trophoblast – Abortion – Ectopic pregnancy – Cytokeratin – Y-chromosome – In situ hybridisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract. With spontaneous abortion the conceptus is often expelled and lost right at the beginning, and uterine curettings then contain only endometrial fragments and clotted blood. Even complete embedding of all available material for histologic examination will not reveal any chorionic villi, and ectopic pregnancy can thus not be excluded. In such cases, the intermediate trophoblast can sometimes still be demonstrated within the endometrial tissue. This highly invasive trophoblast is difficult to identify using conventional staining, but cytokeratin antibodies are reliable markers of this cell type. Using immunohistochemistry, these fetal components could be demonstrated in 27 of 95 specimens (28.5%), proving the intrauterine nature of the aborted pregnancy. In some cases the fetal derivation of intermediate trophoblast was demonstrated by using in situ hybridisation to mark repetitive sequences on the Y-chromosome in the interphase nucleus.
    Notes: Zusammenfassung. Wird bei einem Spontanabort zu Beginn die eigentliche Fruchtanlage ausgestoßen und nicht asserviert, sind im Untersuchungsgut häufig nur noch endometriale Schleimhautfragmente und Blutkoagula anzutreffen. Auch bei vollständiger Aufarbeitung des Gewebes sind oft keine Chorionzotten mehr erkennbar. Somit kann das Vorliegen einer Extrauteringravidität nicht ausgeschlossen werden. Fetales Gewebe in Form des intermediären Trophoblasten (IT) ist manchmal aber noch innerhalb der bei der Kürettage geförderten Dezidua nachzuweisen. Diese invasive Trophoblastkomponente ist wegen ihrer Ähnlichkeit zum Deziduagewebe bei konventioneller Färbung kaum sicher zu identifizieren. Sie kann aber in zuverlässiger Weise immunhistochemisch mit Zytokeratinantikörpern dargestellt werden. Auf diese Weise konnten bei 95 intrauterinen Schwangerschaften ohne Chorionzotten im Abrasionsmaterial in 27 Fällen (28,5%) fetale Anteile in Form des intermediären Trophoblasten festgestellt werden und damit der Beweis einer ehemals intrauterin gelegenen Schwangerschaft erbracht werden. Darüber hinaus wurde an einigen Fällen die fetale Abkunft des IT mittels In-situ-Darstellung des Y-Chromosoms am Interphasenkern bewiesen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002920050021
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...