ISSN:
1524-475X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Percutaneous devices play a central role in modern medicine, but are associated with significant risk of infection. One approach to circumvent this risk is to heal the wound around the device by creating a seal at the skin/device interface. We previously showed, using cell adhesion assay and organ culture model, that surface modification of poly(2-hydroxyethyl methacrylate)[poly(HEMA)] with 1,1′-carbonyldiimidazole (CDI) dramatically enhanced keratinocyte attachment to poly(HEMA). Although the organ culture model addresses epithelial response to an implanted biomaterial, to fully evaluate the cutaneous interaction with biomaterials, we tested implants in a mouse model. Porous poly(HEMA) rods with 40 μm pore size were implanted into two different sites on dorsal skin of forty eight, 6–8 week old male C57BL/10 J mice. Porous poly(HEMA) rods were treated with 1) PBS, 2) CDI, and 3) CDI plus laminin 5. Samples were harvested at 7, 14, and 28 days after surgery and analyzed morphologically using H&E and immunohistochemistry. Implanted rods remained intact and infection free at all time points. Morphological analysis showed diffuse dermal cellular infiltration in all samples and evidence of epidermal integration into the pores of CDI treated poly(HEMA) and CDI plus laminin 5 treated poly(HEMA). Immunohistochemistry showed laminin 5 expression at the skin/poly(HEMA) interface resembling the junctional epithelium of the tooth. Engineering this type of attachment to percutaneous devices is attractive since the tooth is a natural example of percutaneous material, where bacterial invasion of bone is prevented by epithelial attachment.Acknowledgments: NSF EEC 9529161 [University of Washington Engineered Biomaterials (UWEB)], NIH DK 59221, the George F. Odland Endowed Research Fund, and the Marvin and Judy Young Research Fund
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1067-1927.2005.130215k.x
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