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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Chemical research in toxicology 6 (1993), S. 117-124 
    ISSN: 1520-5010
    Source: ACS Legacy Archives
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The microbial biomass and community structure of eight Chinese red soils with different fertility and land use history was investigated. Two community based microbiological measurements, namely, community level physiological profiling (CLPP) using Biolog sole C source utilization tests and phospholipid fatty acid (PLFA) profiles, were used to investigate the microbial ecology of these soils and to determine how land use alters microbial community structure. Microbial biomass-C and total PLFAs were closely correlated to organic carbon and total nitrogen, indicating that these soil microbial measures are potentially good indices of soil fertility in these highly weathered soils. Metabolic quotients and C source utilization were not correlated with organic carbon or microbial biomass. Multivariate analysis of sole carbon source utilization patterns and PLFAs demonstrated that land use history and plant cover type had a significant impact on microbial community structure. PLFAs showed these differences more than CLPP methods. Consequently, PLFA analysis was a better method for assessing broad-spectrum community differences and at the same time attempting to correlate changes with soil fertility. Soils from tea orchards were particularly distinctive in their CLPP. A modified CLPP method, using absorbance readings at 405 nm and different culture media at pH values of 4.7 and 7.0, showed that the discrimination obtained can be influenced by the culture conditions. This method was used to show that the distinctive microbial community structure in tea orchard soils was not, however, due to differences in pH alone.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 156 (1997), S. 149 -156 
    ISSN: 1432-1424
    Keywords: Key words: Na+-Ca2+ exchanger — Sarcolemma — Inhibitory peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. The Na+-Ca2+ exchanger plays an important role in cardiac contractility by moving Ca2+ across the plasma membrane during excitation-contraction coupling. A 20 amino acid peptide, XIP, synthesized to mimic a region of the exchanger, inhibits exchange activity. We identify here amino acid residues important for inhibitory function. Effects of modified peptides on Na+-Ca2+ exchange activity were determined. Exchange activity was assessed as 45Ca2+ uptake into Na+-loaded cardiac sarcolemmal vesicles. We find that the entire length of XIP is important for maximal potency, though the major inhibitory components are between residues 5 and 16. Basic and aromatic residues are most important for the inhibitory function of XIP. Substitutions of arginine 12 and arginine 14 with alanine or glutamine dramatically decrease the potency of XIP, suggesting that these residues play a key role in possible charge-charge interactions. Substitutions of other basic residues with alanines or glutamines had less effect on the potency of XIP. All aromatic residues participate in binding with the exchanger, probably via hydrophobic interactions as indicated by tryptophan fluorescence. A tyrosine is required at position 6 for maximal inhibition and phenylalanine 5 and tyrosine 8 can only be replaced by other aromatic residues. Tyrosine 10 and tyrosine 13 can be replaced with other bulky residues. A specific conformation of XIP, with structural constrains provided by all parts of the molecule, is required for optimal inhibitory function.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' journal of analytical chemistry 364 (1999), S. 782-785 
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A direct chemiluminescence method for the determination of nucleic acids has been developed based on the enhancement of nucleic acids on the chemiluminescence light emission of the reaction between Ru(phen)3 2+(phen = 1,10-phenanthroline) and Ce(IV). Under the optimum conditions, the calibration graphs are linear in the range of 5.0 × 10–8–5.0 × 10–5 g/mL for calf thymus DNA, 8.0 × 10–8–5.0 × 10–5 g/mL for fish sperm DNA and 1.0 × 10–7–3.0 × 10–5 g/mL for yeast RNA, respectively. The limits of detection are 1.0 × 10–8 g/mL for calf thymus DNA, 3.1 × 10–8 g/mL for fish sperm DNA and 5.2 × 10–8 g/mL for yeast RNA, respectively. The final procedure allows the successful determination of calf thymus DNA, fish sperm DNA and yeast RNA in six synthetic samples. This method is simple, rapid and specific.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0789
    Keywords: Key words Acidic forest soil ; Phosphorus ; Coal combustion by-product ; Carbon cycling ; Cellulose ; Microbial biomass ; Liming
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Phosphate rock (PR), limestone, coal combustion by-product (CCBP) high in Ca and high organic manures are potential amendments for increasing agricultural production in the acidic soils of the Appalachian region. The objective of this study was to examine effects of PR, CCBP and cellulose addition on soil microbial biomass in an acidic soil based on the measurement of soil microbial biomass P (P mic) and on the mineralization of organic matter. Application of PR alone or in combination with CCBP increased P mic. The P mic was far less when the soil received PR in combination with limestone than with PR application alone or PR in combination with CCBP. Either CCBP or limestone application alone considerably decreased P mic in the soil due to reduced P solubility. Cellulose addition alone did not increase P mic, but P mic was significantly increased when the soil was amended with cellulose in combination with PR. The decomposition of added cellulose was very slow in the soil without PR amendment. However, mineralization of both native organic matter and added cellulose was enhanced by PR application. Mineralization of organic matter was less when the soil was amended with PR in combination with high rates of CCBP (〉 2.5%) because PR dissolution varied inversely with amount of CCBP addition. Overall, CCBP had no detrimental effect on soil microbial biomass at low application rates, although, like limestone, CCBP at a high rate may decrease P mic in P-deficient soils through its influence on increased soil pH and decreased P bioavailability in the soil. Application of PR to an acidic soil considerably enhanced the microbial activity, thereby promoting the cycling of carbon and other nutrients.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 24 (1997), S. 421-428 
    ISSN: 1432-0789
    Keywords: Key words Seasonal responses ; Microbial biomass C ; Microbial biomass P ; Microbial biomass S ; Nutrient cycling ; Pasture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The response of the soil microbial biomass to seasonal changes was investigated in the field under pastures. These studies showed that over a 9-month period, microbial biomass carbon, phosphorus and sulphur (biomass C, P, S), and their ratios (C:P, C:S, and P:S) responded differently to changes in soil moisture and to the input of fresh organic materials. From October to December (1993), when plant residues were largely incorporated into the soils, biomass C and S increased by 150–210%. Biomass P did not increase over this time, having decreased by 22–64% over the dry summer (July to September). There was no obvious correlation between biomass C, P, and S and air temperature. The largest amounts of biomass C and P (2100–2300μg and 150–190μgg–1 soil, respectively) were found in those soils receiving farmyard manure (FYM or FYM+NPK) and P fertilizer, whereas the use of ammonium sulphate decreased biomass C and P. The C:P, C:S, and P:S ratios of the biomass varied considerably (9–276:1; 50–149:1; and 0.3–14:1, respectively) with season and fertilizer regime. This reflected the potential for the biomass to release (when ratios were narrow) or to immobilize (wide ratios) P and S at different times of the year. Thus, seasonal responses in biomass C, P, and S are important in controlling the cycling of C, P, and S in pasture and ultimately in regulating plant availability of P and S. The uptake of P in the pasture was well correlated with the sum of P in the biomass and soil available pools. Thus, the simultaneous measurement of microbial biomass P and available P provide useful information on the potential plant availability of P.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 32 (2000), S. 500-507 
    ISSN: 1432-0789
    Keywords: Keywords Fumigation-extraction techniques ; Microbial biomass P ; Acid soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  This study aimed to validate the fumigation-extraction method for measuring microbial biomass P in acid soils. Extractions with the Olsen (0.5 M NaHCO3, pH 8.5) and Bray-1 (0.03 M NH4F–0.025 M HCl) extractants at two soil:solution ratios (1 : 20 and 1 : 4, w/v) were compared using eight acid soils (pH 3.6–5.9). The data indicated that the flushes (increases following CHCl3-fumigation) of total P (Pt) and inorganic P (Pi) determined by Olsen extraction provided little useful information for estimating the amount of microbial biomass P in the soils. Using the Bray-1 extractant at a soil:solution ratio of 1 : 4, and analysing Pi instead of Pt, improves the reproducibility (statistical significance and CV) of the P flush in these soils. In all the approaches studied, the Pi flush determined using the Bray-1 extractant at 1 : 4 provided the best estimate of soil microbial biomass P. Furthermore, the recovery of cultured bacterial and fungal biomass P added to the soils and extracted using the Bray-1 extractant at 1 : 4 was relatively constant (24.1–36.7% and 15.7–25.7%, respectively) with only one exception, and showed no relationship with soil pH, indicating that it behaved differently from added Pi (recovery decreased from 86% at pH 4.6 to 13% at pH 3.6). Thus, correcting for the incomplete recovery of biomass P using added Pi is inappropriate for acid soils. Although microbial biomass P in soil is generally estimated using the Pi flush and a conversion factor (k P) of 0.4, more reliable estimates require that k P values are best determined independently for each soil.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 67 (1987), S. 16-26 
    ISSN: 1432-1106
    Keywords: Lateral geniculate nucleus ; Single neurons ; Receptive field ; Patterned background ; Summation ; Disinhibition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the lateral geniculate nucleus (LGN) of paralyzed cats under urethane anaesthesia, an extensive disinhibitory region (DIR) outside the inhibitory surround of the receptive field (RF) was found in both sustained and transient cells. Its extent is comparable to that of McIlwain's periphery effect (1964). The responses to a light spot, flashed into different parts of the RF center, were used to assess the effect of different background patterns, located over the DIR, on responsiveness and receptive field organization. A straight line background cutting across the RF center led to a marked shrinkage in RF size and to a suppression of the center response. In sustained cells, these influences were not due to the light flux of the background, but were mainly due to the spatial property of the line itself. This was demonstrated by comparing the effect of a straight line background with that of a zigzag line or of distributed dots. The light flux for the different patterns and their spatial weighting was the same, so that they differed from each other solely in their form. A straight line background elicited much stronger suppression of the center response and more marked shrinkage of the RF than if the component dots are dispersed over a wider area, but keeping the radial distances of the individual dots from the RF-center constant. The data suggest that the dispersion of the component dots in different backgrounds plays an important role as response amplitude and RF diameter increase proportional to the dispersive area of the background patterns. For transient cells, all backgrounds used showed similar effects on center responses and RF diameter, indicating that for them it was the light flux of the backgrounds rather than their spatial property that caused the effects.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Applied mechanics and materials Vol. 10-12 (Dec. 2007), p. 225-229 
    ISSN: 1662-7482
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Aiming at the characteristics of the product design team under internet environment, theintelligent resource selecting method through price is put forward. Based on this, the evaluationmodel of the product design team under internet environment is established, and meanwhile, thepractical case is introduced to provide an effective method for the optimization of the intelligentresources
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 204-206 (Mar. 1996), p. 641-646 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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