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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 64 (1995), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: We have investigated basic fibroblast growth factor (FGF-2) localization in and release from isolated bovine adrenal chromaffin cells. In contrast to previous reports, we found no evidence of fibroblast growth factor (FGF) storage in catecholamine-containing chromaffin granules. Subcellular fractionation studies did not show enrichment of FGF-2 immunoreactivity in granules, and cholinergic stimulation failed to release FGF-2 into the medium. Our results suggest that adrenal chromaffin cells resemble other FGF-2-synthesizing cell types with respect to FGF storage and secretion.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Cell Biology International Reports 13 (1989), S. 993-1006 
    ISSN: 0309-1651
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1433-0407
    Keywords: Schlüsselwörter Epilepsiechirurgie ; Prächirurgische Diagnostik ; Nichtinvasives Protokoll ; Key words Epilepsy surgery ; Presurgical evaluation ; Noninvasive protocol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary We present a non-invasive epilepsy surgery protocol, which includes EEG-video-monitoring, magnetic resonance imaging (MRI), interictal positron emission tomography (PET) and ictal single photon emission computerized tomography (SPECT). According to this non-invasive protocol 50 of 173 patients with medically intractable focal epilepsy underwent resective surgery. The localization of the epileptogenic zone was based on the congruence of the localizing results of EEG-video-montoring, MRI, interictal PET and ictal SPECT. 46 (92%) of the patients had temporal and 4 (8%) had extratemporal epilepsies. 78% (n=39) of all patients operated according to our non-invasive protocol were postoperatively completely or almost seizure free. Extramesiotemporal resections could be carried out without invasive EEG-recording if the epileptogenic zone was not adjacent to the eloquent cortex. We conclude from our results that in a considerable number of patients with medically intractable particularly temporal focal epilepsies, resective epilepsy surgery can be based on non-invasive EEG-evaluations and the risk of invasive recordings can be avoided.
    Notes: Zusammenfassung Wir stellen ein nichtinvasives Protokoll für die epilepiechirurgische Diagnostik vor, demzufolge bei 50 von 173 Patienten mit pharmakoresistenten fokalen Epilepsien ein resektiver epilepsiechirurgischer Eingriff durchgeführt wurde. Die lokalisatorische Übereinstimmung von Befunden des nichtinvasiven EEG-Video-Monitorings, der Magnet-Resonanz-Tomographie (MRT), der interiktalen Positronen-Emissions-Tomographie (PET) und der iktalen SPECT (Single-Photon-Emissions-Computer-Tomographie) bildete die Grundlage der Fokuslokalisation. Bei 46 (92%) der Patienten lagen temporale und bei 4 (8%) extratemporale Epilepsien vor. 78% (n=39) aller 50 Patienten wurden anfallsfrei oder hatten nur noch sehr selten Anfälle. Extratemporale Resektionen konnten ohne invasive EEG-Untersuchungen erfolgen, wenn umschriebene MRT-Läsionen vorlagen und die Resektionen in ausreichendem Abstand vom eloquenten Kortex durchgeführt werden konnten. Anfallsfreiheit konnte so bei insgesamt 3 der 4 extratemporalen Patienten erreicht werden. Wir folgern aus unseren Ergebnissen, daß bei einer Reihe von ausgewählten Patienten mit pharmakoresistenten fokalen, insbesondere temporalen Epilepsien nichtinvasive EEG-Ableitungen in der prächirurgischen Diagnostik ausreichen und so das Risiko invasiver EEG-Untersuchungen vermieden werden kann.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 296 (1999), S. 229-233 
    ISSN: 1432-0878
    Keywords: Key words Black widow spider venom ; Calcium-independent receptor for α-latrotoxin ; Exocytosis ; G-proteins ; Latrophilin ; Neurexins ; Chromaffin cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The major component of black widow spider venom, α-latrotoxin, triggers massive exocytosis in a variety of neurosecretory cells. An important trigger for exocytosis is the calcium influx via α-latrotoxin-induced channels in biological membranes. However, this mechanism fails to explain exocytosis which occurred in the complete absence of extracellular calcium. Recently, sophisticated biochemical and molecular techniques have led to the discovery of novel α-latrotoxin-binding membrane receptors: neurexins and latrophilin/CIRL (calcium-independent receptor for α-latrotoxin). Neurexins are single transmembrane proteins which bind to α-latrotoxin in a calcium-dependent manner and also interact with the synaptic vesicle protein, synaptotagmin. On the other hand, latrophilin is a seven-transmembrane protein and belongs to the family of G-protein-coupled receptors. The multitude of effects of α-latrotoxin on exocytosis in different cell systems and the nature of its membrane targets are discussed in this article. The molecular details of how α-latrotoxin binding is transduced eventually to exocytosis remain to be elucidated.
    Type of Medium: Electronic Resource
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