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  • 1
    Electronic Resource
    Electronic Resource
    PO Box 1354, 9600 Garsington Road , Oxford OX4 2XG , UK . : Blackwell Science Ltd
    Geophysical prospecting 52 (2004), S. 0 
    ISSN: 1365-2478
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Physics
    Notes: An integrated multiscale seismic imaging flow is applied to dense onshore wide-aperture seismic data recorded in a complex geological setting (thrust belt).An initial P-wave velocity macromodel is first developed by first-arrival traveltime tomography. This model is used as an initial guess for subsequent full-waveform tomography, which leads to greatly improved spatial resolution of the P-wave velocity model. However, the application of full-waveform tomography to the high-frequency part of the source bandwidth is difficult, due to the non-linearity of this kind of method. Moreover, it is computationally expensive at high frequencies since a finite-difference method is used to model the wave propagation. Hence, full-waveform tomography was complemented by asymptotic prestack depth migration to process the full-source bandwidth and develop a sharp image of the short wavelengths. The final traveltime tomography model and two smoothed versions of the final full-waveform tomography model were used as a macromodel for the prestack depth migration.In this study, wide-aperture multifold seismic data are used. After specific preprocessing of the data, 16 frequency components ranging from 5.4 Hz to 20 Hz were inverted in cascade by the full-waveform tomography algorithm. The full-waveform tomography successfully imaged SW-dipping structures previously identified as high-resistivity bodies. The relevance of the full-waveform tomography models is demonstrated locally by comparison with a coincident vertical seismic profiling (VSP) log available on the profile. The prestack depth-migrated images, inferred from the traveltime, and the smoothed full-waveform tomography macromodels are shown to be, on the whole, consistent with the final full-waveform tomography model. A more detailed analysis, based on common-image gather computations, and local comparison with the VSP log revealed that the most accurate migrated sections are those obtained from the full-waveform tomography macromodels. A resolution analysis suggests that the asymptotic prestack depth migration successfully migrated the wide-aperture components of the data, allowing medium wavelengths in addition to the short wavelengths of the structure to be imaged.The processing flow that we applied to dense wide-aperture seismic data is shown to provide a promising approach, complementary to more classical seismic reflection data processing, to quantitative imaging of complex geological structures.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Arginine auxotrophs of the dinitrogen-fixing cyanobacterium Anabaena species strain PCC 7120 were isolated after ultraviolet light mutagenesis and penicillin enrichment. Two of these auxotrophs were complemented by a cosmid gene library of the wild-type strain established in Escherichia coli that was transferred en masse to the mutants by conjugation. The gene complementing one of those mutants was found to complement an E. coli argC mutant. Sequencing analysis of the gene showed that it encodes a 322-residue polypeptide that is homologous to the ArgC protein of E. coli, Bacillus subtilis and Streptomyces clavuligerus and to the C-terminal moiety of the Sac-charomyces cerevisiae ARG5,6 gene product, N-acetylglutamate semialdehyde dehydrogenase. A cysteine residue present in a highly conserved domain in the five proteins is probably located in the active site of the enzyme. Conserved among the ArgC proteins, sequences resembling the primary structure of nucleotide-binding domains are also found. Downstream of the Anabaena argC gene seven nearly perfect repeats of a heptanucleotide (consensus sequence: 5′-CTAATGA-3′) are found.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A nuclease that could be recovered from the supernatant of cultures, as well as from cell-free extracts, of the cyanobacterium Anabaena sp. PCC 7120 was identified as a 29 kDa polypeptide by its ability to degrade DNA after electrophoresis in DNA-containing SDS-polyacrylamide gels. Some clones of a gene library of strain PCC 7120 established in Escherichia coli were found to produce the 29 kDa nuclease. The nucA gene encoding this nuclease was subcloned and sequenced. The deduced polypeptide, NucA, had a molecular weight of 29650, presented a presumptive signal peptide in its N-terminal region and showed homology to the products of the nuc gene from Serratia marcescens and the NUC1 gene from Saccharomyces cerevisiae. The NucA protein from Anabaena itself, or from the cloned nucA gene expressed in E. coli, catalysed the degradation of both RNA and DNA, had the potential to act as an endonuclease, and functioned best in the presence of Mn2+ or Mg2+. An Anabaena nucA insertional mutant was generated which failed to produce the 29 kDa nuclease.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The gene ntcA is required for full expression of proteins subject to ammonium repression in the cyanobacterium Synechococcus. A 3.1 kb DNA fragment able to complement an ntcA mutant was digested with exonuclease III, and deleted fragments of different sizes were tested for complementation of that mutant, allowing the localization of its mutation within a BamHI-HindIII genomic fragment of c. 0.4 kb. Insertion of a chloramphenicol-resistance-encoding gene cassette into both the BamHI and the HindiIII sites of wild-type Synechococcus resulted in a pleiotropic, nitrogen-assimilation-minus phenotype, corroborating the presence of the ntcA gene in that genomic region. Sequencing of DNA in this region showed the presence of an open reading frame that included both the BamHI and the HindIII sites. The ntcA gene product, NtcA, is a protein of 24817 Da which belongs to a family of bacterial transcriptional activators that, among others, includes Crp and Fnr from Escherichia coli. Of special biological significance, it appears, is the presence of a conserved helix-turn-helix motif in the sequence close to the C-terminal end of all the proteins in the family. The gene ntcA is proposed to encode a transcriptional activator of genes subject to nitrogen control in Synechococcus.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : We report on the Raman spectra of white spots formed during frozen storage of shrimp on the shell in Pleoticus muelleri argentine, measured in situ. From Raman analysis this study determined that ikaite is the predominant calcium carbonate crystal formed on the inner shell side, while ikaite crystals are accompanied by significant amounts of calcite on the outer side. Calcite could be a dehydration compound appearing during sample preparation. On the basis of data from Raman spectroscopy, there seem to be no significant protein and/ or chitin conformational changes accompanying the precipitation of ikaite.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Raman spectroscopy and electron microscopy were used to examine the structural properties of sodium dodecyl sulfate-mercaptoethanol-unextractable aggregates formed during the frozen storage of hake muscle. Our results showed that: (a) the unextractable residue consisted of thick filaments, which appeared connected and aggregated, forming a network; (b) the protein backbone adopted a conformational structure rich in β-sheets; and (c) Raman spectroscopy revealed for the 1st time the presence of symmetrical stretching vCH2 bands, whose frequencies are consistent with the presence of lysine-arginine and/or lysine-glutamine/asparagine bridges in the aggregates.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 69 (2004), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : The possibilities of using the stress relaxation test as a nondestructive method to monitor post-mortem textural changes of ice-stored cod have been evaluated. The stress relaxation tests were performed in gutted cod. Fish were compressed by 5% and deformation was kept constant for 60 s. The relaxation curves obtained were fitted to nonlinear and linear regression models. Nonlinear regressions with 2 or 3 exponential terms and the linear regression model could be fitted to the relaxation curve of ice-stored cod, although the three-terms exponential models gave the best results for assessing the quality of ice-stored cod (maximum relative difference ≦ 2% and R 2 〉 0.999). The highest differences were found between pre-rigor and in-rigor states.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0003-2670
    Keywords: Anodic stripping voltammetry ; Copper ; Differential pulse voltammetry ; Multivariate calibration ; Partial least squares ; Standard addition ; Trace metals ; Wine
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 896 (1987), S. 103-108 
    ISSN: 0005-2736
    Keywords: (A. nidulans) ; Nitrite transport ; Nitrogen control
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Archives of Biochemistry and Biophysics 234 (1984), S. 454-459 
    ISSN: 0003-9861
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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