ISSN:
1432-0533
Keywords:
Neuroepithelial differentiation
;
Microcomplement fixation
;
Indirect immunofluorescence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Mouse neonatal brain cell fractions enriched for surface membranes were used as immunogens to produce a heterologous immune serum. Following absorption to remove non-neural anti-mouse activity, this serum demonstrated by microcomplement fixation an anti-brain activity that was completely removed by absorption with neonatal mouse brain or with solid tumors of the mouse transplantable teratoma OTT-6050. Indirect immunofluorescence applied to living monolayer cultures of differentiating teratoma embryoid bodies showed the absorbed serum's reaction with neural cell surfaces only. In material studied with frozen sections, the absorbed serum recognized antigenic sites in all examined areas of both neonatal and adult mouse brain, and only within neuroepithelial cell populations of solid transplants of the teratoma.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00691708
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