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  • 1
    Electronic Resource
    Electronic Resource
    Validation of immunolocalization of the urokinase receptor expression in ductal carcinoma in situ of the breast: comparison with detection by non-isotopic in-situ hybridization (2000)
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Histopathology 36 (2000), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aims: Ductal carcinoma in situ (DCIS) is a pre-invasive form of mammary carcinoma with no microscopic evidence of cancer cell invasion through the basement membrane. However, for initiation of invasion, tumour cells have to acquire and focus proteolytic activity on to the cell surface in order to infiltrate the surrounding extracellular matrix. The receptor (uPA-R or CD87) for the serine protease urokinase-type plasminogen activator (uPA) plays a central role in invasion and metastasis. This study was performed to determine and localize m-RNA and protein of uPA-R in ductal carcinoma in situ of the breast.〈section xml:id="abs1-1"〉〈title type="main"〉Methods and resultsWe analysed uPA-R mRNA and protein expression by in-situ hybridization and immunohistochemistry, respectively, in 50 formalin-fixed, paraffin-embedded specimens of DCIS. Three different antibodies were used to stain cell-associated uPA-R; chicken polyclonal antibody (pAb) HU277 and monoclonal antibodies (mAb) IID7 and 3936. In all cases, myoepithelial and stromal cells reacted with either antibody. Especially, reaction of macrophage-like cells with mAb 3936 resulted in a well-marked and bright staining. Applying mAb IID7, in 46 of the 50 breast specimens tumour cells showed a positive immunoreaction. Likewise pAb HU277 stained tumour cells in 40 of the 50 cases, whereas mAb 3936 reacted with only 24 of the 50 tissue sections. Endothelial cells were marked by both mAb IID7 and pAb HU277 (46/50 and 35/50, respectively); mAb 3936 did not label at all. All of the cell types stained by mAb IID7 and pAb HU277 also displayed reactivity with uPA-R mRNA-specific antisense oligonucleotides in in-situ hybridization.〈section xml:id="abs1-2"〉〈title type="main"〉ConclusionsOur results reveal the presence of the tumour invasion-related receptor for the protease uPA not only in invasive ductal breast carcinoma but also in different types of DCIS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.2000.00899.x
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  • 2
    Electronic Resource
    Electronic Resource
    Panniculitis in a patient with chronic myelogenous leukaemia treated with imatinib (2003)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 149 (2003), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2003.05594.x
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  • 3
    Electronic Resource
    Electronic Resource
    Cutaneous large B-cell lymphoma of the leg masquerading 
as a chronic venous ulcer (2002)
    Oxford, UK : Blackwell Science, Ltd
    British journal of dermatology 146 (2002), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We report on a 74-year-old female patient with a primary cutaneous CD20+, diffuse large cell B-cell lymphoma of the lower leg resembling a chronic non-healing leg ulcer. There was no evidence of systemic involvement on computed tomography (CT) scans of the chest, abdomen and pelvis; a slightly enlarged lymph node in the right groin showed dermatopathic lymphadenopathy on histology and immunohistochemistry. Involvement of the bone marrow and peripheral blood was ruled out by punch biopsy and fluorescent activated cell sorter (FACS) analysis of the blood, respectively. Therapeutic anti-CD20 monoclonal antibody rituximab was given at 375 mg m−2 i.v. once weekly for 7 weeks, without adverse effects, resulting in a minor improvement in the centre of the ulcerated tumour. Unfortunately, the response was not maintained, and after 7 weeks of treatment the patient started to develop new tumour lesions at the border of the ulcer. Local radiotherapy was started and combined photon and electron beam irradiation induced complete remission of the B-cell lymphoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.0007-0963.2001.04520.x
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  • 4
    Electronic Resource
    Electronic Resource
    Plötzlicher Herztod bei infantiler Systemvaskulitis (1998)
    Springer
    Monatsschrift Kinderheilkunde 146 (1998), S. 969-971 
    Details
    ISSN: 1433-0474
    Keywords: Schlüsselwörter Kawasaki-Syndrom ; Koronarthrombose ; Herzinfarkt ; Key words Kawasaki-syndrome ; Coronary thrombosis ; Myocardial infarction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A 23 month old boy was admitted to hospital because of anemia, mainly truncal rash and unclear pain. Previously he had shown fever, conjunctivitis and desquamations of hands and feet. Laboratory findings were moderate leucocytosis, thrombocytosis, anemia, and an elevation of ESR and gamma-globulins. No bacterial infection was found. The ECG showed signs of a myocardial ischemia. The sonography of the heart detected an aneurysm of the left coronary artery. The boy died of a sudden cardiac arrest. The autopsy verified the diagnosis of Kawasaki-syndrome and revealed two coronary aneurysms blocked by thrombosis and a necrotic panarteriitis. Discussion: The main cause of death in Kawasaki disease is myocardial infarction resulting from thrombotic occlusion in a coronary aneurysm. This case is of interest because the infantile systemic vasculitis is associated with an acute Paravo B 19 virus infection, which so far has been reported in only one case.
    Notes: Zusammenfassung Ein 23 Monate alter Junge wurde wegen ausgeprägter Anämie, stammbetontem Exanthem und unklarer Schmerzsymptomatik stationär aufgenommen. Aus der Vorgeschichte waren Fieber, Konjunktivitis und eine grob lamelläre Schuppung der Hände und Füße bekannt. Laborchemisch fielen eine mäßige Leukozytose, eine Thrombozytose, eine Anämie, eine stark beschleunigte BKS und eine Erhöhung der γ-Globuline auf. Ein bakterieller Infekt konnte ausgeschlossen werden. Das EKG wies Zeichen einer Myokardischämie auf. Die Sonographie des Herzens ergab ein Koronararterienaneurysma der linken Kranzarterie. Der Junge verstarb unter den Zeichen des Sekundenherztods. Die Obduktion sicherte die Diagnose eines Kawasaki-Syndroms mit beidseits thrombotisch verschlossenen Koronararterienaneurysmen bei nekrotisierender Panarteriitis. Diskussion: Der Myokardinfarkt, ausgelöst durch einen thrombotischen Verschluß eines Koronararterienaneurysmas, ist die Haupttodesursache der Kawasaki-Erkrankung. Die Besonderheit dieses Falls liegt in einer Assoziation der infantilen Systemvaskulitis und einer frischen Parvo-B-19-Virus-Infektion, die bisher nur 1mal beschrieben wurde.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001120050353
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  • 5
    Electronic Resource
    Electronic Resource
    Urokinase receptor localization in breast cancer and benign lesions assessed by in situ hybridization and immunohistochemistry (1998)
    Springer
    Histochemistry and cell biology 110 (1998), S. 27-32 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The serine protease urokinase-type plasminogen activator (uPA) mediates cancer invasion and metastasis by binding to a cell surface receptor (uPA-R, CD87) on both tumor and stromal cells. In the present study we assessed uPA-R distribution in formalin-fixed, paraffin-embedded breast cancer specimens (n=50) and benign lesions (n=10) by immunohistochemistry employing a newly developed polyclonal chicken antibody to uPA-R (pAb HU277) in parallel with established monoclonal antibody (mAb) 3936 to uPA-R. In addition, uPA-R mRNA synthesis was investigated by in situ hybridization. In all of the sections analyzed, macrophage-like cells reacted with either antibody type. In 22 of the 50 cancer specimens, tumor cells reacted with pAb HU277 in contrast to mAb 3936 which only stained 9 of the 22 positive cases. Nevertheless, in 49 of the 50 cases, uPA-R mRNA was detected in cancer and in stromal cells by in situ hybridization suggesting posttranscriptional regulation of uPA-R expression in breast cancer cells. In 18 of 50 cases, uPA-R mRNA was also visualized in blood vessel lining endothelial cells by in situ hybridization and applying pAb HU277 in 14 of these 18 cases by immunohistochemistry. mAb 3936 did not stain any endothelial cells. pAb HU277 reacted with the breast gland epithelial cells of benign lesions as well, in contrast to mAb 3936 which did not. As for the cancer tissue, in benign lesions, endothelial cells were sporadically stained by pAb HU277. This antibody, but not mAb 3936, also stained myoepithelial cells in intraductal areas of invasive breast carcinoma. The results presented demonstrate the usefulness of pAb HU277 in locating uPA-R in tumor and normal cells with high sensitivity in formalin-fixed, paraffin-embedded breast tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050261
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  • 6
    Electronic Resource
    Electronic Resource
    Großes Brunner-Adenom im Bulbus duodeni – ein Fallbeispiel (1998)
    Springer
    Der Chirurg 69 (1998), S. 1388-1390 
    Details
    ISSN: 1433-0385
    Keywords: Key words: Brunner's gland adenoma ; Duodenum ; Diagnosis ; Surgical treatment. ; Schlüsselwörter: Brunner-Adenom ; Duodenum ; Diagnose ; chirurgische Therapie.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Benigne proliferative Veränderungen der Brunner-Drüsen machen bis zu 10 % der gutartigen Tumoren des Duodenums aus. Große Polypen bzw. Adenome der Brunner-Drüsen sind jedoch sehr selten. Es wird der Fall eines ungewöhnlich großen Adenoms der Brunner-Drüsen (Größe: 12 × 5 × 2,5 cm) vorgestellt. Die klinische Symptomatik bestand aus isolierten, epigastrischen Beschwerden und postprandialem Völlegefühl. In der bildgebenden Diagnostik wurde der Verdacht auf einen Duodenalwandtumor oder Pankreaskopftumor geäußert. Das Adenom wurde durch eine Duodenotomie und Polypektomie operativ entfernt. Proliferative Veränderungen der Brunner-Drüsen des Duodenums manifestieren sich entweder als diffuse oder als lokalisierte noduläre Hyperplasie oder als Brunner-Adenom. Aufgrund ihrer submucösen Lage entgehen sie häufig der Diagnostik durch endoskopische Biopsien. In der Literatur sind bisher nur 14 Fälle maligner Entartung der Brunner-Drüsen beschrieben worden. Da es sich in der Regel um sehr kleine Tumoren handelt, können die meisten Fälle durch eine endoskopische Polypektomie sowohl diagnostiziert als auch behandelt werden. Größere Tumoren können, wie in diesem Fallbeispiel, eine chirurgische Excision erforderlich machen.
    Notes: Summary. Benign, proliferative changes of the Brunner's gland are very rare and account for about 10 % of duodenal bulb neoplasias. The authors present a case of Brunner's gland adenoma of unusual dimensions (12 × 5 × 2.5 cm). The patient presented with vague epigastric discomfort as isolated symptoms. In this case we performed surgical treatment, including a duodenotomy and polypectomy. Because they are localized in the submucosa small, superficial endoscopic biopsies may fail to confirm the diagnosis. Malignancy seems to occur only very rarely, with only 14 cases reported in the literature. As the majority of Brunner's adenomas are quite small, endoscopic polypectomy will confirm the diagnosis and cure the condition in most cases. Large symptomatic adenomas may require surgical resection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001040050591
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