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  • 1
    ISSN: 1432-0428
    Keywords: Key words Glutathione ; γ-glutamylcysteine synthetase ; thiol transport ; erythrocytes ; cytotoxicity ; non-insulin-dependent diabetes mellitus ; K562 cells.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glutathione functions to scavenge oxidants or xenobiotics by covalently binding them and transporting the resulting metabolites through an adenosine 5′-triphosphate-dependent transport system. It has been reported that the intracellular concentration of glutathione decreases in diabetes mellitus. In order to elucidate the physiological significance and the regulation of anti-oxidants in diabetic patients, changes in the activity of the glutathione-synthesizing enzyme, γ-glutamylcysteine synthetase, and transport of thiol [S-(2,4-dinitrophenyl)gluta- thione] were studied in erythrocytes from patients with non-insulin-dependent diabetes and K562 cells cultured with 27 mmol/l glucose for 7 days. The activity of γ-glutamylcysteine synthetase, the concentration of glutathione, and the thiol transport were 77 %, 77 % and 69 %, respectively in erythrocytes from diabetic patients compared to normal control subjects. Treatment of patients with an antidiabetic agent for 6 months resulted in the restoration of γ-glutamylcysteine synthetase activity, the concentration of glutathione, and the thiol transport. A similar impairment of glutathione metabolism was observed in K562 cells with high glucose levels. The cytotoxicity by a xenobiotic (1-chloro-2,4-dinitrobenzene) was higher in K562 cells with high glucose than in con- trol subjects (50 % of inhibitory concentration; 300 ± 24 μmol/l vs 840 ± 29 μmol/l, p 〈 0.01). Expression of γ-glutamylcysteine synthetase protein was augmented in K562 cells with high glucose, while enzymatic activity and expression of mRNA were lower than those in the control subjects. These results suggest that inactivation of glutathione synthesis and thiol transport in diabetic patients increases the sensitivity of the cells to oxidative stresses, and these changes may lead to the development of some complications in diabetes mellitus. [Diabetologia (1995) 38: 201–210]
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0428
    Keywords: Glutathione ; γ-glutamylcysteine synthetase ; thiol transport ; erythrocytes ; cytotoxicity ; non-insulin-dependent diabetes mellitus ; K562 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glutathione functions to scavenge oxidants or xenobiotics by covalently binding them and transporting the resulting metabolites through an adenosine 5′-triphosphate-dependent transport system. It has been reported that the intracellular concentration of glutathione decreases in diabetes mellitus. In order to elucidate the physiological significance and the regulation of anti-oxidants in diabetic patients, changes in the activity of the glutathione-synthesizing enzyme, γ-glutamylcysteine synthetase, and transport of thiol [S-(2,4-dinitrophenyl)glutathione] were studied in erythrocytes from patients with non-insulin-dependent diabetes and K562 cells cultured with 27 mmol/l glucose for 7 days. The activity of γ-glutamylcysteine synthetase, the concentration of glutathione, and the thiol transport were 77%, 77% and 69%, respectively in erythrocytes from diabetic patients compared to normal control subjects. Treatment of patients with an antidiabetic agent for 6 months resulted in the restoration of γ-glutamylcysteine synthetase activity, the concentration of glutathione, and the thiol transport. A similar impairment of glutathione metabolism was observed in K562 cells with high glucose levels. The cytotoxicity by a xenobiotic (1-chloro-2,4-dinitrobenzene) was higher in K562 cells with high glucose than in control subjects (50% of inhibitory concentration. 300±24 Μmol/l vs 840±29 Μmol/l, p〈0.01). Expression of γ-glutamylcysteine synthetase protein was augmented in K562 cells with high glucose, while enzymatic activity and expression of mRNA were lower than those in the control subjects. These results suggest that inactivation of glutathione synthesis and thiol transport in diabetic patients increases the sensitivity of the cells to oxidative stresses, and these changes may lead to the development of some complications in diabetes mellitus.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Chemical Physics Letters 176 (1991), S. 526-530 
    ISSN: 0009-2614
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Chemical Physics Letters 204 (1993), S. 219-225 
    ISSN: 0009-2614
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Chemical Physics Letters 171 (1990), S. 433-438 
    ISSN: 0009-2614
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 99 (1995), S. 1655-1659 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Totals of 2.67 x 105 and 7.56 x 105 juvenile red sea bream of three size groups (10, 20 and 40 mm t.l.) marked with a fluorescent substance in the otolith were released in News Bay, Oita Prefecture, Japan, in July 1987 and June 1988, respectively; the aim was to estimate growth and mortality of different developmental stages.Of fish released in 1987 and 1988, 10 618 and 4413, respectively were recaptured during those two years. Released fish remained in the bay until the end of summer, and afterwards migrated out towards open waters. Fish of the 40-mm group released in 1987 grew to over 200 mm t.l. in one year. Mean growth rate for 19 days after release was higher in the 40-mm group (0.87 mm day−1) than in the 20-mm group (0.74mm day−1). Survival rates over 19 days were 59.0 and 10.1 % for 40-mm and 20-mm fish, respectively, in 1987, and those over 30 days were 69.2, 3.3 and 0.0% for 40-mm, 20-mm and 10-mm fish, respectively, in 1988.Cannibalism was indicated by the presence of marked otoliths for 20-mm fish in the stomachs of a few 40-mm individuals recaptured 2 days after release. Size-dependent growth and size-selective mortality were both noted in juvenile red sea bream, i.e. the relative size differential between larger and smaller individuals was maintained in the period between marking and recapture, and mortality was inversely proportional to size.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Tetrahedron Letters 33 (1992), S. 6647-6650 
    ISSN: 0040-4039
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 26 (1993), S. 223-225 
    ISSN: 1434-6079
    Keywords: 36.40 ; 34.90
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Collision-induced reactions of size-selected cluster anions, (CO2) n − and (N2O)nO− with He and Kr atoms were studied at collision energies from 0.1 to 2.0 eV (center-of mass) by means of a tandem mass-spectrometer equipped with a pair of octapole ion guides. The dominant process was evaporation of the constituent molecules from the parent cluster ion. The absolute cross section for the evaporation was measured as functions of the size of the parent cluster ion and the collision energy. The reaction was explained by collisional excitation of the parent cluster ion followed by its unimolecular dissociation. The observed cross sections which correspond to those for the collisional excitation agree with those calculated in terms of charge-induced dipole and induced dipole-induced dipole interactions between the parent cluster ion and the target atom. The distributions of the product ions resulting from the unimolecular dissociation were reproduced by a simple calculation based on RRK theory. In the collision of (CO2) n − , the cross sections for (CO2) 10 − and (CO2) 14 − were significantly small and their abundances in the product ion distributions were particularly large. These findings indicate that (CO2) 10 − and (CO2) 14 − are stable species. On the other hand, stable species in (N2O)nO− was found to be (N2O)5O−.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 31 (1994), S. 187-189 
    ISSN: 1434-6079
    Keywords: 36.40.+d
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Collision of Ar cluster ions, Ar n + (n=3−16), with He and Ne atoms was investigated by use of mass spectroscopic techniques. The cross sections for the production of Ar n′ + (n′〈n) were measured as functions of the size of the parent cluster ion and the collision energy (0.1–10 eV in the center-of-mass frame). These results were analyzed in the scheme of hard-sphere spectator collision with RRK theory. It was concluded that the reaction proceeds via collisional excitation of the parent cluster ion and following sequential loss of the constituent Ar atoms.
    Type of Medium: Electronic Resource
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