ISSN:
1420-908X
Keywords:
Key words: Gelatinase — Matrix metalloproteinase — Neutrophil — Inflammation — Guinea pig
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract. Objective: Matrix metalloproteinase (MMP)-9 plays an important role in neutrophil extravasation and migration by its ability to degrade the major components of basement membrane. To evaluate the expression of neutrophil MMP-9 under inflammatory conditions, we examined the levels of MMP-9 and its mRNA in neutrophils of glycogen-induced peritoneal inflammation.¶Materials and Methods: Male Hartley guinea pigs weighing 250—300 g were intraperitoneally injected with 0.17% glycogen solution, and 13—15 h after the injection, blood and peritoneal neutrophils were isolated. The levels of MMP-9 and its mRNA were analyzed by gelatin zymography and Northern blotting, respectively. Furthermore, MMP-9 activities in the peritoneal supernatants were measured.¶Results: MMP-9 level in peritoneal neutrophils was essentially the same as that in blood neutrophils, although peritoneal neutrophils were assumed to have extracellularly released MMP-9 from the granules during infiltration into the peritoneal cavity. Interestingly, MMP-9 mRNA was expressed more abundantly in peritoneal neutrophils than in blood neutrophils (p〈0.01). Moreover, MMP-9 levels in blood and peritoneal neutrophils were reduced to 30—45% of non-treated controls by actinomycin D (500 μg/kg) or cycloheximide (10 mg/kg)-treatment (p〈0.05). In contrast, MMP-9 activity increased in the peritoneal supernatants of glycogen-injected animals was not significantly affected by actinomycin D- or cycloheximide-treatment. In addition, when blood neutrophils of non-injected animals were stimulated with 10-7 M N-formyl-Met-Leu-Phe, 10 μg/ml lipopolysaccharide, 10 ng/ml phorbol 12-myristate 13-acetate, 10-8 M IL-8 or 100 U/ml tumor necrosis factor-α, expression of MMP-9 mRNA was markedly increased (p〈0.05).¶Conclusions: The present observations indicate that MMP-9 gene is transcribed, and MMP-9 protein is synthesized in neutrophils during glycogen-injected peritoneal inflammation. Moreover, it is likely that MMP-9 protein in the peritoneal supernatants is mostly derived from preformed MMP-9 which is stored in the neutrophil granules and extracellularly released during infiltration of neutrophils into the peritoneal cavity. Finally, the transcription of MMP-9 gene can be upregulated in neutrophils by stimulation with inflammatory mediators, even after neutrophils have been matured.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s000110050559
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