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  • 1
    Electronic Resource
    Electronic Resource
    Purification and characterization of RNA polymerase II resistant to .alpha.-amanitin from the mushroom Agaricus bisporus (1979)
    s.l. : American Chemical Society
    Biochemistry 18 (1979), S. 795-803 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00572a009
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  • 2
    Electronic Resource
    Electronic Resource
    The insensitivity of mushroom nuclear RNA polymerase activity to inhibiton by amatoxins (1978)
    Springer
    Archives of microbiology 118 (1978), S. 317-319 
    Details
    ISSN: 1432-072X
    Keywords: RNA polymerase ; Transcription ; Amatoxins ; Nuclei ; Poisonous mushrooms ; In vitro RNA synthesis ; Agricus bisporus ; Amanita phalloides ; Amanita muscaria ; Achlya ambisexualis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nuclear fractions isolated from Amanita phalloides, Amanita muscaria and Agaricus bisporus were subjected to in vitro RNA synthesis assays in the presence of various concentrations of amatoxins. The mushroom nuclei were highly insensitive to inhibition by amatoxin when compared to assays of nuclear fractions isolated from the Oömycete fungus, Achlya ambisexualis and from rabbit brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429124
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  • 3
    Electronic Resource
    Electronic Resource
    The developmental patterns of lysosomal enzyme activities during Ca2+-induced sporangium formation in Achlya bisexualis (1976)
    Springer
    Archives of microbiology 109 (1976), S. 289-294 
    Details
    ISSN: 1432-072X
    Keywords: Achlya ; Ribonuclease ; Development ; Sporangium ; Lysogome ; Inhibitors poly(A)+RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The present paper describes intracellular changes in ribonuclease specific activity during Ca2+-induced sporangium formation in the water mold Achlya bisexualis. The enzymes undergo a decrease in activity prior to crosswall formation followed by an increase in activity during spore cleavage. As spore discharge occurs the RNase activity again decreases. A large percentage of the nuclease activity is associated with a lysosomal-like fraction of the cell, but there is also considerably activity associated with nuclear and microsomal fractions. Addition of cycloheximide or actinomycin D at various times during development prevents further decrease or increase in the enzyme activity. Mixing of cell extracts from different developmental stages provides evidence that inhibitors or activators of the enzyme activity are not responsible for the activity levels evident at the different stages. There is a change in the total levels of presumptive mRNA during Ca2+-induced sporangial formation which appears to be associated with the patterns of RNase activity. Utilizing total cellular RNA and Poly(A)+ RNA with the crude ribonuclease preparations, no substrate specificity could be ascertained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446640
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  • 4
    Electronic Resource
    Electronic Resource
    The developmental patterns of lysosomal enzyme activities during Ca++-induced sporangium formation in Achlya bisexualis (1975)
    Springer
    Archives of microbiology 102 (1975), S. 9-12 
    Details
    ISSN: 1432-072X
    Keywords: Lysosomes ; α-Mannosidase ; Sporangium ; Achlya ; Cycloheximide ; Actinomycin ; Differentiation ; Morphogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The present paper describes intracellular changes in α-mannosidase specific activity during Ca++-induced sporangium formation in the water mold Achlya bisexualis. The enzyme, which is concentrated in a cellular fraction with lysosome-like characteristics, undergoes a four-fold increase during sporangium differentiation. Addition of cycloheximide (100 μg/ml) or actinomycin D (10 μg/ml) at any time during the developmental sequence prevents further increase in the enzyme activity. These data suggest that coincident RNA and protein synthesis are essential for the accumulation of enzyme activity. Mixing of cell extracts from different developmental stages provides evidence that activators or inhibitors of the enzyme activity are not responsible for the enzyme activity evident at the different stages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428338
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  • 5
    Electronic Resource
    Electronic Resource
    A comparison of the nucleotide sequence of the cerato-ulmin gene and the rDNA ITS between aggressive and non-aggressive isolates of Ophiostoma ulmi sensu lato, the causal agent of Dutch elm disease (1996)
    Springer
    Current genetics 29 (1996), S. 168-173 
    Details
    ISSN: 1432-0983
    Keywords: Key words  Cerato-ulmin ; Gene structure ; Ophiostoma ulmi ; rDNA ITS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Little genetic information exists comparing aggressive and non-aggressive isolates of the causal agent of Dutch elm disease, Ophiostoma ulmi. Two genetic elements were compared between the subgroups. The cerato-ulmin cu gene product has been associated with disease symptoms. Nucleotide-sequence analysis of cu and the internal transcribed spacer (ITS) region of the rDNA were made from three aggressive and three non-aggressive isolates of the pathogen. Our results suggested uniformity within, and unique differences between, subgroups. Differences were detected for cu in the promoter, coding, and transcription termination regions. Sequence data for the ITS clearly distinguish the subgroups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050032
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  • 6
    Electronic Resource
    Electronic Resource
    A comparison of the nucleotide sequence of the cerato-ulmin gene and the rDNA ITS between aggressive and non-aggressive isolates ofOphiostoma ulmi sensu lato, the causal agent of Dutch elm disease (1996)
    Springer
    Current genetics 29 (1996), S. 168-173 
    Details
    ISSN: 1432-0983
    Keywords: Cerato-ulmin ; Gene structure ; Ophiostoma ulmi ; rDNA ITS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Little genetic information exists comparing aggressive and non-aggressive isolates of the causal agent of Dutch elm disease,Ophiostoma ulmi. Two genetic elements were compared between the subgroups. The ceratoulmincu gene product has been associated with disease symptoms. Nucleotide-sequence analysis ofcu and the internal transcribed spacer (ITS) region of the rDNA were made from three aggressive and three non-aggressive isolates of the pathogen. Our results suggested uniformity within, and unique differences between, subgroups. Differences were detected forcu in the promoter, coding, and transcription termination regions. Sequence data for the ITS clearly distinguish the subgroups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221581
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Plasmid RNA polymerase-like mitochondrial sequences inAgaricus bitorquis (1996)
    Springer
    Current genetics 29 (1996), S. 370-376 
    Details
    ISSN: 1432-0983
    Keywords: Plasmid ; RNA polymerase ; Mitochondrion ; Agaricus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A linear mitochondrial plasmid, pEM, found in certain isolates of the basidiomyceteAgaricus bitorquis potentially encodes virus-like DNA and RNA polymerases. Mitochondrial DNA fromAgaricus bisporus that hybridizes to an internal region of pEM contains a fragmented and potentially non-functional version of the carboxy terminal end of the plasmid RNA polymerase. In this study, we present the sequence of the corresponding region of mitochondrial DNA fromA. bitorquis. This sequence contained the same region of the plasmid RNA polymerase gene as was reported for the mitochondrial DNA ofA. bisporus, and the level of similarity between theA. bisporus andA. bitorquis mitochondrial sequences was much higher than the level of similarity between either mitochondrial sequence and the plasmid. We propose that this plasmid RNA polymerase-like sequence was present in theAgaricus mitochondrial genome before the divergence ofA. bisporus andA. bitorquis, and thus is unlikely to be a recent derivative of the plasmid pEM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02208618
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  • 8
    Electronic Resource
    Electronic Resource
    A gene associated with filamentous growth in Ophiostoma novo-ulmi has RNA-binding motifs and is similar to a yeast gene involved in mRNA splicing (2000)
    Springer
    Current genetics 37 (2000), S. 94-103 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsDimorphism ; Insertional mutagenesis ; Ophiostoma novo-ulmi ; RNA-binding proteins (RNPs)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The COL1 gene was isolated from Ophiostoma novo-ulmi using the techniques of insertional mutagenesis and plasmid rescue. Sequence analyses suggest that the COL1 gene encodes a unique protein of 826 amino acids with consensus-type RNA-binding domains, most similar to a putative protein from Schizosaccharomyces pombe which resembles the C-terminus of the Saccharomyces cerevisiae U4/U6 splicing factor PRP24. Disruption of the COL1 gene produced the yeast-like col1 mutant. The inability of the mutant to synthesize the COL1 gene product was confirmed by transcript analysis. Transformation of the col1 mutant with the COL1 gene restored the wild phenotype and production of the 4.0-kb mRNA. The results from this study demonstrate that the COL1 RNA-binding protein is associated with filamentous growth in O. novo-ulmi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050015
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  • 9
    Electronic Resource
    Electronic Resource
    Plasmid-like DNAs in the commercially important mushroom genus Agaricus (1984)
    Springer
    Current genetics 8 (1984), S. 615-619 
    Details
    ISSN: 1432-0983
    Keywords: Agaricus ; Plasmid-like DNAs ; Mitochondrial DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two unique plasmid-like DNA components were localized in isolated mitochondria of the commercially important mushroom genus Agaricus: pEM (7.35 ± 0.15 kilobases) and pMPJ (3.65 ± 0.15 kilobases). These DNA moieties were linear; pEM possessed regions of terminal inverted repeated sequences. No homology was detected between pEM or pMPJ DNA and the nuclear or mitochondrial genomes. No homology existed between pEM and pMPJ. This suggests independent replication of pEM and pMPJ. Restriction endonuclease digests indicated that pEM consisted of two components (pEM1 and pEM2) with uniquely different restriction sites and copy number.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395707
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  • 10
    Electronic Resource
    Electronic Resource
    The mitochondrial DNAs of Agaricus: heterogeneity in A. bitorquis and homogeneity in A. brunnescens (1985)
    Springer
    Current genetics 9 (1985), S. 127-132 
    Details
    ISSN: 1432-0983
    Keywords: Agaricus ; Mitochondrial DNA ; Restriction pattern polymorphism ; Restriction endonuclease analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNAs were isolated from four cultivated strains of the commercial two-spored mushroom Agaricus brunnescens (bisporus) and from ten isolates of the four spored mushroom Agaricus bitorquis. Digestion of the fungal mitochondrial DNA with restriction endonucleases yielded numerous fragments. Summation of the fragment sizes gave a mitochondrial DNA size of 98.3 ± 2.4 kilobases (kb) (64.9 x 106 daltons) for A. brunnescens. The size of the mitochondrial DNA ranged from 148.5 ± 10.8 kb (98.0 x 106 daltons) to 176.3 ± 12.0 kb (116.4 x 106 daltons) for A. bitorquis. The restriction patterns, produced by a variety of endonucleases, were identical for all four isolates of A. brunnescens. The ten isolates of A. bitorquis demonstrated extensive restriction pattern heterogeneity and have been tentatively assigned into four groups. Approximately 60% of the A. bitorquis mitochondrial DNA restriction fragments show sequence homology with A. brunnescens mitochondrial DNA based on DNA — DNA hybridizations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436960
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