ISSN:
1432-0983
Keywords:
ARS
;
mtDNA
;
Replicon dosage
;
Yeast plasmids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The rep1 region of the yeast mitochondrial genome, a putative replication origin, contains a weak autonomously replicating sequence (ARS). Nucleotidesequence and deletion analyses have identified two 11-base pair ARS consensus sequences, numerous near matches to the ARS core, and a region of curvature that may contribute to ARS function. Based on the amplified nature of petite-derivative mitochondrial DNA encompassing this locus, we have constructed plasmids containing an increasing dosage of ARS elements. The rep1 ARS element can have an additive effect on plasmid stability when present either as a tandem dimer or as an unlinked pair. However, the presence of a third ARS copy does not further enhance plasmid stability. These results indicate that measurable dosage effects can be defined only in circumstances where weak ARS elements are employed, and that plasmid maintenance within yeast cells is saturable and varies among the different sequences promoting replication.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00352013
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