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  • 1
    Electronic Resource
    Electronic Resource
    Glucose oxidase biosynthesis using immobilized mycelium of Aspergillus niger (1991)
    Springer
    World journal of microbiology and biotechnology 7 (1991), S. 379-384 
    Details
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A simple method is described for the immobilization of Aspergillus niger GIV-10 which produces an extracellular glucose oxidase. A. niger conidia were immobilized on sintered glass Raschig rings, pumice stones or polyurethane foam. Mycella growing out from the spores produced extracellular glucose oxidase: the highest production was with the pumice stone carrlers. This technique facilitates the growth of the filamentous cultures in the spongy structure of a support with continuous accumulation of biomass. After 24 to 36 h, a culture liquid with 2.7 to 3.1 U of glucose oxidase/ml was obtained. This procedure also made possible repeated batch enzyme production and as many as 25 subsequent 24-h batches could be fermented by using the same carrier with only a small loss of glucose oxidase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329406
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Intensification of glucose oxidase synthesis with aspergillus niger by the way of multistage mutagenization (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 371-376 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Conidia of glucose oxidase active Aspergillus niger G 13 strain were subjected to the fourth-stage mutagenization using different combinations of mutagens in each stage (stage I - UV radiation + nitrosomethylurea, stage II - UV radiation + ethyleneimine, Stage III - acryflavine, stage IV - UV radiation + N-methyl-N′-nitrosoguanidine). In all, 400 strains which showed higher glucose oxidase activity with our own diffusion data method (were isolated out of 5208 colonies after mutagenization). Nine most active mutants were tested in the submerged culture determining the activity of glucose oxidase in the post-culture liquid which increased from 10.7 to nearly 30%.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100412
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Influence of the mycelium growth conditions on the production of amylolytic, proteolytic and pectinolytic enzymes by Aspergillus niger C (1989)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 9 (1989), S. 355-361 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Various nitrogen and carbon sources, as well as natural products, were examined as inducers of the production of amylases, proteases and pectinases by A. niger C. A. niger C grown on wheat bran extract medium provided culture supernatants with the highest enzymatic activities. Some culture conditions, e.g. pH, medium temperature and time period of cultivation, were optimalized to improve the growth and enzymes biosynthesis by A. niger C.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370090411
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    Paper (German National Licenses)
    Fulltext
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