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  • 1
    Electronic Resource
    Electronic Resource
    Biochemical characterization of 1,25(OH)2D3 receptors in chick embryonal duodenal cytosol (1982)
    Springer
    Calcified tissue international 34 (1982), S. 265-269 
    Details
    ISSN: 1432-0827
    Keywords: 1,25(OH)2D3 Receptor ; Chicken Duodenal Cytosol ; Chicken Embryo ; Affinity ; 1,25(OH)2D3 Concentration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary This study presents measurements of serum vitamin D metabolites, calcium and phosphorus as well as measurements of the equilibrium dissociation constant for duodenal 1,25(OH)2D3 receptor in 15-, 18-, 19-, and 20-day chick embryos in comparison to that in 1- and 118-day-old chicks and to vitamin D-deficient chicks. The present results showed that: (a) serum 1,25(OH)2D and 24,25(OH)2D levels rise from 15 and 18 to days 19 and 20 of embryonic development while serum phosphate levels are stable; (b) serum calcium levels rise at hatching to adult levels; (c) the duodenal 1,25(OH)2D3 receptor is detectable in 15-day-old embryo and has a Kd similar to that of 118-day-old vitamin D-replete chicks; and (d) the activity of 1,25(OH)2D3 receptor in chick duodenal cytosol is maximal at hatching.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02411248
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  • 2
    Electronic Resource
    Electronic Resource
    A synthesis of (+)-nonactic acid by means of the sulfur-ylide rearrangement
    Amsterdam : Elsevier
    Tetrahedron 48 (1992), S. 79-88 
    Details
    ISSN: 0040-4020
    Keywords: (+)-methyl nonactate ; (+)-nonactic acid ; dimethyl α-diazomalonate ; rhodium acetate ; tetrahydro-2-furanthione
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0040-4020(01)80580-9
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro and in vivo studies of the production of placental proteins (HCG, SP1, PPs) in human choriocarcinoma cell lines (1987)
    Springer
    Archives of gynecology and obstetrics 241 (1987), S. 111-120 
    Details
    ISSN: 1432-0711
    Keywords: Placental proteins ; Human choriocarcinoma cells ; Production in vitro and in vivo ; Radioimmunoassay and immunoperoxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The production of human chorionic gonadotropin (hCG), pregnancy-specific beta-1 glycoprotein (SP1), and the various placental tissue proteins (PP4, PP5, PP10, PP11, PP12, MP1) was examined in human choriocarcinoma cell lines using radioimmunoassay (RIA) and an immunoperoxidase (PAP) staining method. In 6 gestational and 2 nongestational choriocarcinoma cell lines, hCG was produced in vitro. A significant heterogeneity of hCG production was noted among the cell lines. Production of hCG in the xenograft tumors was consistent with the hCG production of the same cell lines in vitro. SP1 was demonstrated both in the culture supermatants and the xenograft tumors of 3 cell lines, and the production was found to be inversely correlated with the hCG production of the cell lines. Positive staining for PP4 and MP1, and weakly positive staining for PP5, PP10, and PP12 was found in the xenograft tumors by immunoperoxidase (PAP) stain, but the staining for PP11 was negative. These placental proteins showed positive staining in portions of choriocarcinoma cells. These results indicate that placental proteins are not equally produced in heterogeneous choriocarcinoma cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00931231
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    Paper (German National Licenses)
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