ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Pectinesterase was purified from the culture medium of Aspergillus japonicus completely free from pectin depolymerizing enzymes. The purified enzyme was able to convert high-methoxyl pectin into low-methoxyl pectin capable of forming strong gels with calcium ion. The maximum gel strength of enzyme-demethylated pectin was obtained at methoxyl content of 6.3%, calcium concentration of 41.8 mg/g of pectin and pH 3.5. The low-methoxyl pectin, however, formed weaker gels at low concentrations of calcium ion and low pH. DEAE-cellulose column chromatography indicated that the enzyme-demethylated pectin was homogeneous with respect to methoxyl groups of the molecules. It was suggested that this homogeneity may be probably responsible for high gel strength of the low-methoxyl pectin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1979.tb03847.x
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