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  • 1
    Electronic Resource
    Electronic Resource
    Beziehungen zwischen mechanischer und ventilatorischer Inhomogenität (1967)
    Springer
    Lung 135 (1967), S. 270-276 
    Details
    ISSN: 1432-1750
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02093557
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  • 2
    Electronic Resource
    Electronic Resource
    Book review (1996)
    Springer
    European radiology 6 (1996), S. 209-209 
    Details
    ISSN: 1432-1084
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00181149
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  • 3
    Electronic Resource
    Electronic Resource
    RNA pseudoknots
    Amsterdam : Elsevier
    Current Opinion in Structural Biology 2 (1992), S. 327-333 
    Details
    ISSN: 0959-440X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0959-440X(92)90221-R
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  • 4
    Electronic Resource
    Electronic Resource
    Shape dependences in pulsed laser–matter interaction (1995)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 102 (1995), S. 7124-7130 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: It is shown that the shape dependence of final state probability distributions in pulsed laser–atom or molecule interactions has a simple explanation in the theory of quantum transport of Floquet states. More precisely, the predictions of a quasienergy resonance model—complex poles in the quasienergy plane produced by quantum transport and populated by state-mixing at near-degeneracies—are successfully compared with numerical solutions of the initial value problem of the Schrödinger equation for a Morse-potential model. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.469106
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  • 5
    Electronic Resource
    Electronic Resource
    Molecules interacting with short, intense laser pulses: The dynamical role of quasistationary states (1996)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 104 (1996), S. 8943-8949 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: A theoretical framework for the description of the pulse-induced reaction dynamics of molecules interacting with short, intense laser pulses is derived. Born–Oppenheimer adiabaticity is formulated in terms of quasistationary Floquet states; its violation and the structure of the potential mixing electronic levels, and thus effecting this violation, is discussed; it is shown that, on the basis of quasistationary states, this potential is (to a very good approximation) nonvanishing only around near degeneracies in the quasienergy spectrum where it has a simple two-level structure. Ab initio calculations showing the effectivity of this scheme are performed for the H2 molecule. Particular emphasis is put on bond-softening reactions; a new mechanism is described. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.471628
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  • 6
    Electronic Resource
    Electronic Resource
    A two-site monoclonal antibody ELISA for the quantification of group V allergens in grass extracts (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 24 (1994), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A two-site solid-phase enzymimmunoassay was used for the quantification of group V allergen in grass pollen extracts in mass units. The assay is based on the monoclonal antibodies (MoAbs), 1D11 and 3B2 which recognize different epitopes on the standard Phl p V. The MoAb-ELISA is very sensitive (15 100ng ml Phl p V)and highly specific for group V allergens. Six pollen extracts of different grasses demonstrated parallel binding curves. The group V content ranged between 73 and 673 μg/ml in the extracts. The International Standard of Phleum pratense (IS 82 520) contains 400 μg/nil Phl p V. A good correlation was observed between the group V content and RAST inhibition, with the exception of Poa pratensis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1994.tb00986.x
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  • 7
    Electronic Resource
    Electronic Resource
    Monoclonal antibody immunoassay for quantitative analysis of group V allergens in grass pollen extracts (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A two-site monoclonal antibody (MoAb) ELISA has been developed for the quantification of the Phleun pratense major allergen, Phl p V. The assay is based on two MoAbs which recognize different non-overlapping epitopes on the Phi p V molecule; one antibody (1D11) was immobilized on the solid phase and the other (3B2) was biotinylated. An affinity-purified Phi p V preparation (purity of 95%) was used as standard. The assay has a sensitivity of 10 ng/ml of allergen and is suitable for the detection of group V allergen in aqueous grass extracts. The specificity of the assay was investigated with 14 grass pollen and five non-grass pollen extracts. Different levels of group V allergen were delected in extracts of grasses, but not in non-grasses. The assay gives a good correspondence with allergenic activity of extracts as determined by ELISA inhibition using serum pool of allergic patients. The results indicate that the two-site MoAb ELISA could be very useful in the standardization of allergenic extracts from grass pollen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1993.tb00362.x
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  • 8
    Electronic Resource
    Electronic Resource
    Detection of crossreactive determinants in grass pollen extracts using monoclonal antibodies against group IV and group V allergens (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Monoclonal antibodies (MoAb) to defined allergens of P. pratense were raised. Five of them were selected for detailed studies by means of immunoblotting after SDS–PAGE and IEF of extracts from P. pratense and L. perenne. Three antibodies (1D11, 3B2, 2D1) recognize structures with mol. wt of 29 and 34 kD and pI of 4.4-7.7, corresponding to group V allergens. Two other MoAbs (2D8, 3C4) are directed against strong basic structures with a mol. wt of 50 kD and pI of 7.7-9.9 according to group IV allergens. The specificity of antibodies was supported by direct ELISA with purified group V and IV allergens. The isolated allergens were characterized before by SDS-PAGE and CIE and that allergenicity was detected with sera of patients with allergic rhinitis. Using our selected MoAbs crossreactive epitopes on group V and IV allergens have been excluded. Our antibodies have been used to detect crossreactivity in 14 grass pollen extracts. The evaluation of the pollen extracts has been performed by enzyme immunoassay (EIA) inhibition. One MoAb (3C4) is able to recognize group IV allergens in all grass species analysed whereas the MoAb 2D8 seems to identify group IV structures in selected grasses only. Binding to conserved structures of group V has been proved for MoAb 1D11. Other group V specific MoAbs (2D1, 3B2) identify similar, however incomplete, spectra. These results have been confirmed also by the dot immunobinding assay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1993.tb02484.x
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  • 9
    Electronic Resource
    Electronic Resource
    Quantification of group 5 grass pollen allergens in house dust (2000)
    Oxford BSL : Blackwell Science Ltd
    Clinical & experimental allergy 30 (2000), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: It is widely known and accepted that grass pollen is a major outdoor cause of hay fever. However, it is of virtual importance for grass pollen allergic patients with symptoms all the year round to know the concentration of grass pollen allergens in their homes.〈section xml:id="abs1-2"〉〈title type="main"〉ObjectiveThe main objective of this study was to quantify the amount of grass pollen allergen in mass units (μg Phl p 5) in dust settled indoors and to detect the distribution of allergenic activity in different sampling locations of homes. Furthermore, we studied the seasonal fluctuation of allergen content in dust samples.〈section xml:id="abs1-3"〉〈title type="main"〉MethodsWe adapted the two site binding assay for detection of group 5 grass pollen allergens in samples from randomly selected homes in Hamburg (n = 371), Erfurt (n = 396), Hettstedt (n = 353), Zerbst (n = 289) and Bitterfeld (n = 226), Germany. Dust samples were collected from floor of living room (LR), bedroom (BR) or children's room (CR) and mattress (MA) during period of June 1995 to August 1998. The amount of the major grass group 5 allergens was detected in μg/g dust.〈section xml:id="abs1-4"〉〈title type="main"〉ResultsPhl p 5 was detected in 67% of the samples analysed (n = 4760). The range was between undetectable (〈 0.03 μg/g dust) and 81 μg/g dust. Phl p 5 levels were significantly higher in the dust from LR (geometric mean 0.117 μg/g dust) or BR/CR floors (geometric mean 0.098 μg/g dust) than in mattresses (geometric mean 0.043 μg/g dust). We observed seasonal fluctuation of indoor Phl p 5 levels with peak in June but also annual differences. Thus Phl p 5 content indoors reflects also the different quantities of pollen counts of annual courses. During pollination period we found two times higher Phl p 5 levels (0.172 μg/g dust, P 〈 0.001) than outside of grass pollination season (0.095 μg/g dust). The indoor Phl p 5 levels outside of season seem to be independent of pollination before. We suppose that settled pollen grains or allergenic material from outdoor particles carried indoors via footwear and clothes accumulates in house dust.〈section xml:id="abs1-5"〉〈title type="main"〉ConclusionAlthough we not known how the allergens in settled dust are equilibrated with those in the air, the considerable high level of Phl p 5 in indoor dust even during periods when no grass pollen is present in the atmosphere may be an important cause of pollen-allergy symptoms outside of season.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2000.00926.x
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  • 10
    Electronic Resource
    Electronic Resource
    Monitoring of the Cooperative Unfolding of the sunY Group I Intron of Bacteriophage T4
    Amsterdam : Elsevier
    Journal of Molecular Biology 234 (1993), S. 331-346 
    Details
    ISSN: 0022-2836
    Keywords: RNA folding ; group I intron ; ribozyme activation ; self-splicing ; tertiary interaction
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/jmbi.1993.1590
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