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  • 1
    Electronic Resource
    Electronic Resource
    Homogeneous mono-125I-insulins (1980)
    Springer
    Diabetologia 19 (1980), S. 546-554 
    Details
    ISSN: 1432-0428
    Keywords: 125I-insulin ; insulin ; radioiodination ; iodate ; lactoperoxidase ; monoiodotyrosine ; diiodotyrosine ; A chain ; B chain ; tyrosine-A14 ; tyrosine-A19 ; insulin immunoassay ; radiochemical purity ; radioimmunochemical stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mono-125I-(Tyr A19)-insulin (Mono *A19) was prepared by iodinating MC porcine insulin with 125I in acid medium using iodate (as oxidizing agent), followed by anion-exchange chromatography. Mono-125I-(Tyr A14)-insulin (Mono *A14) was prepared by iodinating MC porcine insulin with 125I, using H2O2/lactoperoxidase at neutral pH, followed by anion-exchange chromatography. The specific radioactivities were in the ranges of 120–200 and 220–300 mCi/mg for Mono *A19 and Mono *A14, respectively. Analyses of the intramolecular distributions of 125I demonstrated that the preparations were 97–98% radiochemically pure. In both preparations, 98–99% of the radioactivity was capable of binding to insulin antibodies for up to 6 months of storage of the tracers. The IRI concentration decreased with the duration of storage. The greatest observed fall in IRI concentration was 70%. The time course could be explained by the assumption that the disintegration of a 125I-nucleus destroys the immunoreactivity of the insulin molecule in which the decay occurs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253183
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Human proinsulin standards (1984)
    Springer
    Diabetologia 27 (1984), S. 414-415 
    Details
    ISSN: 1432-0428
    Keywords: Human proinsulin ; radioimmunoassay ; standards
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two new batches of pancreatic human proinsulin have been compared with biosynthetic human proinsulin. Standards of these three proinsulin preparations were made on the basis of quantitative amino-acid analyses and compared in two proinsulin radioimmunoassays with a proinsulin standard prepared 14 years ago. The curves of the new standards were superimposable. However, they differed considerably from the curve of the old standard which proved to be only one-third of the strength of the new standards, thereby leading to a threefold over-estimation of proinsulin concentrations when the old standard is used. We conclude that the new standards should replace previously used standards.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304860
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    Paper (German National Licenses)
    Fulltext
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