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  • 1
    Electronic Resource
    Electronic Resource
    Postpolio syndrome: poliovirus persistence is involved in the pathogenesis (1999)
    Springer
    Journal of neurology 246 (1999), S. 472-476 
    Details
    ISSN: 1432-1459
    Keywords: Key words Postpolio syndrome ; Reverse transcriptase ; polymerase ; chain reaction ; Poliovirus ; persistence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The pathogenesis of the postpolio syndrome (PPS) remains unclear. In this study we looked for poliovirus (PV) persistence in the CSF of 20 patients with PPS, in a control group including 20 patients with unrelated neurological diseases, and in 7 patients with stable poliomyelitis sequelae. CSF samples and sera were examined using reverse transcriptase–polymerase chain reaction (RT-PCR) for the detection of PV or other enterovirus genomes; this assay allows the detection from as little as 1 fg viral RNA. Sequencing of amplified products from 5 patients was performed. PV genomic sequences were detected in the CSF of 11 of 20 patients with PPS and in none of the control group. Sequencing in the 5′ untranslated region confirmed the presence of mutated PV sequences. These findings suggest that PPS is related to the persistence of PV in the central nervous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004150050386
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  • 2
    Electronic Resource
    Electronic Resource
    ARS sequences in homologous and heterologous ADE2 loci are capable of promoting autonomous replication of plasmids in Schwanniomyces occidentalis (2000)
    Springer
    Current genetics 37 (2000), S. 298-303 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsSchwanniomyces occidentalis ; ADE2 gene ; ARS element ; 2 μm ori ; ARS1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have analyzed ARS elements linked to homologous and heterologous ADE2 loci functioning in Schwanniomyces occidentalis. We have identified a region of the ADE2 locus of S. occidentalis which promotes autonomous replication of plasmids in S. occidentalis cells. This region is within 385 bp preceding the ATG codon of the S. occidentalis ADE2 gene. It contains sequences similar to ARS core consensus sequences, ARS boxes, and a potential transcription activator binding site characterized in Saccharomyces cerevisiae. The ADE2 gene of S. cerevisiae was found to complement the ade2 mutation in S. occidentalis cells and the 5′ UTR region of this gene is capable of supporting autonomous replication of plasmids in S. occidentalis. Furthermore, we confirmed that the origin of replication of the 2 μm plasmid and the ARS1 sequence of S. cerevisiae are also functional in S. occidentalis cells. Plasmids carrying either ARS, the SwARSA element of S. occidentalis, the ARS linked to the ADE2 gene of S. cerevisiae, and the ARS1 sequence or the 2 μm ori, were found to be maintained in S. occidentalis cells as episomal monomers or oligomers. However, their stability was low as already reported for the ARS in S. occidentalis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050531
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  • 3
    Electronic Resource
    Electronic Resource
    Secretion of invertase from Schwanniomyces occidentalis (1997)
    Springer
    Biotechnology letters 19 (1997), S. 623-627 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Invertase synthesis in Schwanniomyces occidentalis is regulated by catabolite repression and is derepressed by raffinose and low concentrations of glucose. Efficiency of a carbon source in derepression of invertase is dependent upon the type of culture medium: either raffinose in a rich medium or a low concentration of glucose in a yeast minimal medium. The kinetics of derepression can be modulated by changing the carbon source. When cells are grown in a rich medium with 0.5% raffinose as the sole carbon source, Schwanniomyces occidentalis secretes 80 times more invertase than Saccharomyces cerevisiae grown in the same conditions. About 50% of the total amount of invertase produced by Schwanniomyces occidentalis is secreted in the extracellular medium in contrast to Saccharomyces cerevisiae where only 6 to 15% of the protein is secreted in the medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018326512461
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation of auxotrophic mutants of the methylotrophic yeastCandida boidinii and determination of its ploidy (1992)
    Springer
    Antonie van Leeuwenhoek 62 (1992), S. 167-171 
    Details
    ISSN: 1572-9699
    Keywords: Candida boidinii ; mutagenesis ; auxotrophic mutant ; ploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Auxotrophic mutations in the methylotrophic yeast strainCandida boidinii 11Bh were induced by different mutagens and their combinations (nitrosoguanidine, UV light, HNO2+UV). Majority of the mutants obtained carried defects in histidine, arginine, proline and/or adenine biosynthetic pathways. His- mutants were distributed into four complementation groups using the protoplasts fusion technique. Ploidy determination ofCandida boidinii 11Bh was performed by measuring its DNA content and by following its survival after chemical mutagens treatment. The DNA content of this strain was found to be similar to that of aSaccharomyces cerevisiae diploid strain. Also the kinetics of survival ofCandida boidinii cells indicate thatCandida boidinii 11Bh is a diploid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582576
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  • 5
    Electronic Resource
    Electronic Resource
    Yeast sequencing reports. Sequence analysis of the ADE2 gene coding for phosphoribosylaminoimidazole carboxylase in schwanniomyces occidentalis (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 1289-1293 
    Details
    ISSN: 0749-503X
    Keywords: Schwanniomyces occidentalis ; gene ADE2 ; sequencing ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have determined the nucleotide sequence of a 3·3 kb fragment containing the gene (ADE2) encoding phosphoribosylaminoimidazole carboxylase (AIRC) from the yeast Schwanniomyces occidentalis. Translation of a 1671 bp open reading frame predicts a protein of 557 amino acids which has significant homology to AIRC from Saccharomyces cerevisiae and Schizosaccharomyces pombe. The 5′ untranslated region of the S. occidentalis gene contains a sequence corresponding to the consensus binding site of the S. cerevisiae transcription regulatory proteins GCN4, BAS1 and BAS2. The ADE2 gene nucleotide sequence has received the GenBank Accession Number U23210.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320111309
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    Paper (German National Licenses)
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