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  • 1
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; fusion proteins ; nasopharyngeal carcinoma ; pUC plasmid vectors ; prediction of antigenic sites
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Gene 44 (1986), S. 353-359 
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; dihydrofolate reductase gene ; eukaryotic expression vector ; nasopharyngeal carcinoma
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Der Chirurg 71 (2000), S. 374-380 
    ISSN: 1433-0385
    Keywords: Keywords: Viral hepatitis – Hepatitis A – Hepatitis B – Hepatitis C – Hepatitis D – Hepatitis E – Epidemiology – Serological diagnosis – Vaccination. ; Schlüsselwörter: Virushepatitis – Hepatitis A – Hepatitis B – Hepatitis C – Hepatitis D – Hepatitis E – Epidemiologie – Serodiagnostik – Impfung.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Die Virushepatitiden gehören weltweit zu den wichtigsten Infektionskrankheiten. Sie sind vor allem in tropischen und subtropischen Gebieten weit verbreitet, spielen aber auch in den Industrienationen Nord- und Westeuropas und den USA immer noch eine wichtige Rolle. In Deutschland liegt die Zahl der pro Jahr mit Virushepatitiserregern infizierten Personen über 100000. Während Hepatitis A und E faecal-oral übertragen werden, erfolgt die Übertragung der Hepatitis B, C und D parenteral. Die Mehrzahl aller in Deutschland registrierten Hepatitis-A-Infektionen sind im Ausland erworben. Die Hepatitis B kommt immer noch als nosokomiale Infektion vor; der größere Anteil aller Hepatitis-B-Infektionen in Deutschland wird aber wohl sexuell übertragen. Zur serologischen Abklärung einer Virushepatitis stehen uns eine Reihe von empfindlichen Verfahren zum Nachweis spezifischer Antikörper bzw. Antigene zur Verfügung, die eine sichere Diagnose meist aus einer einzigen Serumprobe erlauben. Eine zuverlässige Prophylaxe einer Hepatitis A und B ist durch Totimpfstoffe möglich.
    Notes: Abstract. Viral hepatitis is one of the most important infectious diseases worldwide. It is frequent in tropical and subtropical areas, but also plays still a significant role in the industrialized countries of Northern and Western Europe and the USA. In Germany more than 100 000 people are infected by viral hepatitis each year. Whereas hepatitis A and E are transmitted fecal-orally, hepatitis B, C and D are transmitted by the parenteral route. Most hepatitis A infections seen in Germany are acquired abroad. Hepatitis B is still a nosocomial disease; the majority of infections in Germany, however, seems to be transmitted sexually. For the serological diagnosis of viral hepatitis a battery of sensitive tests for specific antibodies and antigens is available; usually testing of one serum sample allows an unambiguous diagnosis. Prophylaxis of hepatitis A and B is possible using safe and efficient vaccines.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 52 (1996), S. 299-300 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Conclusions Our results indicate that the choice of the probe for ELOSA is of major concern. In our panel we had seven sera which contained about 100 molecules/ml and further five sera which contained less than 1000 molecules/ml. Most of them were detected by the long probe but not by the short probe. When PCR for the S-or PreS-gene was included it was possible to detec all 24 HBV-positive sera (not shown) by ELOSA. The reliable lower quantification limit for the long probe is 250 molecules/ml and for the short probe 2500 molecules/ml. Surprisingly, chemiluminescence did not produce better qualitative or quantitative results. The data suggest that the usage of several replicates allows relative quantification in most cases. One possible drawback we see is the hybridization efficiency. Six of our positive samples showed great differences between the number of target molecules suggested by agarose gel electrophoreses or by hybridization (Southern blot or ELOSA). All of them contained more than 106 molecules/ml. For these cases and for the samples where the short probe and the long probe gave discordant result (2 cases) we think that competitive PCR will be the method of choice, but in most cases ELOSA with the long probe gives reliable results and is highly sensitive.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Infection 17 (1989), S. 70-76 
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 343 gesunde Erwachsene wurden mit fünf verschiedenen Chargen einer rekombinanten Hepatitis-B-Vakzine geimpft. 340 (99,1%) Impflinge bildeten Antikörper gegen Hepatitis B Oberflächenantigen (anti-HBs). Frauen und jüngere Personen wiesen höhere Anti-HBs-Werte auf. Alle Anti-HBs-positiven Personen entwickelten Antikörper gegen die allen Hepatitis-B-Virus-Subtypen gemeinsame Determinante „a“. Die Verträglichkeit des Impfstoffs war sehr gut, schwerere Nebenwirkungen wurden nicht beobachtet. Die Persistenz von Anti-HBs konnte bei 130 Personen drei Jahre und bei 15 Personen vier Jahre lang nach der ersten Impfung verfolgt werden. 21,7% bzw. 32,3% der Impflinge hatten nach dieser Zeit keine schützenden Anti-HBs-Titer mehr. Die Persistenz von Anti-HBs erwies sich als deutlich abhängig von der maximalen Anti-HBs-Konzentration, wobei die Kinetik, mit der Anti-HBs absank, relativ konstant war. Wiederimpfung von Personen, deren Anti-HBs-Spiegel auf Werte von 10 IU/l oder darunter abgesunken waren, führte zu einer sofortigen Immunantwort. Ein Vergleich dieser Befunde mit den Ergebnissen, die mit dem aus Plasma hergestellten Impfstoff erzielt wurden, zeigte keine größeren Unterschiede zwischen beiden Impfstoffen.
    Notes: Summary Three hundred and forty-three healthy adults were vaccinated with five different lots of recombinant hepatitis B vaccine. Three hundred and forty (99.1%) individuals produced antibodies against hepatitis B surface antigen (anti-HBs). Peak anti-HBs concentrations were significantly higher in females and younger individuals. All anti-HBs positive individuals developed antibodies to the common determinant “a” of HBsAg. The vaccine was well tolerated, without severe side reactions. Persistence of anti-HBs was followed in 130 individuals for 3, and in 15 for 4 years after the first vaccination of these two groups. 21.7% and 32.3%, respectively, no longer had protective levels of anti-HBs after this time. The persistence of anti-HBs was dependent on peak anti-HBs levels, with consistent kinetics of anti-HBs decline. Revaccination of individuals whose specific antibody levels had fallen below 10 IU/l led to a prompt anti-HBs response. Comparison with individuals vaccinated with plasma-derived hepatitis B vaccine revealed no substantial differences between the two vaccines.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Infection 14 (1986), S. 100-101 
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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