ISSN:
1573-5028
Keywords:
plant gene expression
;
5-methylcytosine
;
5-azacytidine
;
A. tumefaciens
;
T-DNA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have optimized conditions for demethylation of the genome and induction of a silent, hypermethylated T-DNA gene (ipt) by 5-azacytosine (5-azaCyt) derivatives in a suspension culture of tobacco cells. In this system, 5-azacytidine (5-azaC) is more effective in causing genomic demethylation and ipt gene induction than 5-azaCyt or 5-azadeoxycytidine (5-azadC). A single treatment with 2.5 μM 5-azaC resulted in a maximal level of ipt gene induction without inhibiting cell growth. However, we could not reduce the level of genomic methylation below approximately 2/3 of that found in untreated controls, even after extensive 5-azaC treatment. Furthermore, remethylation of the genome occurred after removal of 5-azaC. The use of 5-azaC as an inducer of silent plant genes is discussed, along with differences in the response of plant and animal genomes to demethylating agents.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00017581
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