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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für Herz-, Thorax- und Gefässchirurgie 12 (1998), S. 221-225 
    ISSN: 0930-9225
    Keywords: Schlüsselwörter Vorhofseptumdefekt – Interventioneller Verschluß– Echokardiographie ; Key words Atrial septal defect – transcatheter closure – echocardiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Surgical closure of atrial septal defects can nowadays be performed without preoperative cardiac catheterization. For a transcatheter closure, however, x-ray exposure has been indispensable. We report a closure of an atrial septal defect under transesophageal echocardiography without fluoroscopy. A nine year old girl with an 11 mm atrial septal defect centrally located in the oval fossa was elected for transcatheter closure. Doppler-echocardiographically estimated Qp/Qs was 1.7:1. Oxymetric Qp/Qs was 1.6:1. After sedation with midazolam and propofol a diagnostic and interventional catheterization was performed without fluoroscopy. Under transesophageal echocardiography, the defect was sized over the wire with a 5F balloon catheter. The distance to the right pulmonary veins was 10 mm, to the coronary sinus and to the mitral valve 8 mm, respectively. Under transesophageal echocardiography, an 11 mm Amplatzer Septal Occluder was placed into the defect. Complete closure was achieved and no complications were encountered. We conclude that in selected cases with an atrial septal defect located in the oval fossa and clear-cut echocardiographic findings, an Amplatzer Septal Occluder can be safely deployed under echocardiographic guidance alone.
    Notes: Zusammenfassung Der operative Verschluß eines Vorhofseptumdefekts (ASD) kann heutzutage ohne präoperative Katheteruntersuchung durchgeführt werden. Für den interventionellen Verschluß unter Durchleuchtung ist jedoch eine Strahlenexposition unvermeidbar. Wir berichten über einen interventionellen ASD-Verschluß allein unter transösophagealer Ultraschallkontrolle. Ein neun Jahre altes Mädchen mit einem 11 mm großen ASD vom Secundum-Typ wurde zum interventionellen ASD-Verschluß vorgestellt. Das Shuntvolumen über den Defekt wurde Doppler-echokardiographisch mit Qp/Qs=1,7:1 und oxymetrisch mit Qp/Qs=1,6:1 bestimmt. Unter Sedierung mit Midazolam und Propofol wurde sowohl die Katheterdiagnostik als auch die Intervention ohne Durchleuchtung durchgeführt. Die Defektgröße wurde unter transösophagealer Echokardiographie mit einem 5F Ballonkatheter ermittelt: Der Defekt war 11 mm groß, der Abstand zu den rechten Lungenvenen betrug 10 mm, zum Koronarsinus und zur Mitralklappe jeweils 8 mm. Unter transösophagealer Echokardiographie konnte komplikationslos ein 11 mm großer Amplatzer Septal Occluder in den Defekt plaziert werden. Es bestand kein Restshunt. Der hier berichtete Fall demonstriert, daß die Technik des interventionellen ASD-Verschlusses inzwischen so ausgereift ist, daß sowohl Diagnostik, Sizing als auch Implantation eines transvenös eingebrachten Amplatzer Septal Occluders ganz ohne Röntgenstrahlung möglich ist.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 77 (2000), S. 3057-3059 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We prepared Josephson junctions made of YBa2Cu3O7 on symmetric 24° LaAlO3 bicrystal substrates. For better coupling of high-frequency radiation, we structured the contacts with logarithmic-periodic antennas made of YBa2Cu3O7. Characterization was done by measuring the voltage–current characteristics to determine the critical current IC, the normal-state resistance RN, the noise parameter Γ, and the Stewart–McCumber parameter βC as predicted by the resistively and capactively shunted junction model (RCSJ). The junctions showed excellent RCSJ-like behavior with ICRN products of up to 2.0 mV at 10 K and 220 μV at 77 K. To demonstrate the possibility to detect high-frequency radiation up to the THz regime, we used the Josephson junction as a detector for radiation from a far-infrared laser and we reconstructed the spectra by Hilbert spectroscopy. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1246
    Keywords: Comet assay ; Reproducibility ; DNA single-strand breaks ; Peripheral mononuclear leukocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The aim of the reported study was to investigate the reproducibility of the single-cell gel electrophoresis (SCGE) assay in the determination of DNA single-strand breaks (SSBs) and to estimate the statistical requirements when the SCGE assay is used for the detection of genotoxicity in humans. In human peripheral mononuclear leukocytes (PMLs), we repeatedly measured the rate of SSBs after in vitro incubation of cells for 1 h at 4°C in phosphate buffered saline (PBS, basal) or 10 μM or 50 μM H2O2 (induced). Intra-assay variation was determined from cryopreserved PMLs of a single donor. To assess intrasubject and intersubject variation, PMLs of ten healthy, nonsmoking subjects (aged 19–37 years) were tested 5–9 times. Cryopreserved cells revealed a mean coefficient of variation of 18% (PBS) and 7%–9% (H2O2). There were statistically significant differences between individuals in the rate of SSBs after incubation in PBS (P 〈 0.01), 10 μM H2O2 (P 〈 0.001), and 50 μM H2O2 (P 〈 0.001). The range of interindividual variability was 26% for basal and 12%–13% for induced SSBs, and the coefficient of intraindividual variation was 18%–72% (PBS) and 7%–23% (H2O2). Neither basal nor induced rates of DNA damage were related to gender or age. Estimates of the minimum detectable effects were based on these observed sources of variability (power 90%, level of significance 5%, assumed sample size 50). With two different groups, a difference of 31% in basal SSBs or 12% in induced SSBs would be detectable. Repeated measurement within one group could detect a difference of 26% in basal and 9% in induced SSBs. In summary, the SCGE assay appears to be suitable for the detection of single-strand breaks, e.g., in biomonitoring or environmental medicine, and the statistical requirements could be derived from our analysis of the sources of variability.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 53 (1966), S. 592-592 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-234X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 36 (1966), S. 381-384 
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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