ISSN:
1573-8272
Keywords:
β-1
;
4-xylanase
;
Bacillus amyloliquefaciens
;
cation exchanger
;
fluidized bed adsorption
;
low bed expansion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract β-1,4-Xylanase, produced extracellularly by Bacillus amyloliquefaciens MIR 32, was isolated directly from the culture broth by adsorption on a cation exchanger, Amberlite IRC-50, in fluidized bed with a low degree of expansion. The enzyme was eluted from the adsorbent by increase in pH, with a recovery of 82.3% and purification of 5.3 fold. About 99.99% of the colony forming units, 82% of the contaminating neutral protease activity, and 100% of the reducing sugars present in the crude feedstock were removed at the end of the purification cycle.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008174513826
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