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  • 1
    Electronic Resource
    Electronic Resource
    The role of the crystal rotation axis in experimental three- and four-beam phase determination (1986)
    [S.l.] : International Union of Crystallography (IUCr)
    Acta crystallographica 42 (1986), S. 178-184 
    Details
    ISSN: 1600-5724
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The geometry of four-beam diffraction and procedures for generating it systematically are described. These utilize relatively simple Renninger-type experimental arrangements. The four reciprocal-lattice points involved in each four-beam interaction are located at the comers of rectangles or symmetrical trapezoids in reciprocal space. One of the sides, or a diagonal, of each such quadrilateral serves as the axis of the azimuthal rotation of the crystal. Experiments designed to compare the relative merits of different types of rotation axes have been carried out. It is found that axes of twofold (or higher) symmetry provide advantages over alternate arrangements for experimental phase determination. Four-beam interactions are then generated systematically and in greater abundance than in all other n-beam interactions combined (n 〉 2). Such interactions usually provide stronger phase indications than comparable three-beam interactions. The experiments also showed that, although the phase of an 'invariant' quartet is clearly invariant to the choice of unit-cell origin, it is not necessarily invariant to a change of rotation axis from one two-fold axis to another.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108767386099579
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  • 2
    Electronic Resource
    Electronic Resource
    The FUR1 gene of Saccharomyces cerevisiae: cloning, structure and expression of wild-type and mutant alleles
    Amsterdam : Elsevier
    Gene 88 (1990), S. 149-157 
    Details
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; nucleotide sequence enzymatic activities ; uracil phosphoribosyltransferase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(90)90026-N
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  • 3
    Electronic Resource
    Electronic Resource
    Calcium-independent phospholipid / diolein-dependent phosphorylation of a soluble ovarian M"r 80 000 substrate protein: Biochemical characteristics
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 1054 (1990), S. 285-296 
    Details
    ISSN: 0167-4889
    Keywords: (Ovaria) ; Effector ; Protein kinase ; Protein phosphorylation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4889(90)90099-Y
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  • 4
    Electronic Resource
    Electronic Resource
    Regulation of the pyrimidine salvage pathway by the FUR1 gene product of Saccharomyces cerevisiae (1991)
    Springer
    Current genetics 19 (1991), S. 333-337 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Pyrimidine salvage pathway ; Semi-dominant mutants ; FUR1 ; Uracil phosphoribosyl transferase ; Regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Saccharomyces cerevisiae, the protein encoded by the FUR1 gene is absolutely required for the expression of uracil phosphoribosyl transferase activity. The occurrence of semi-dominant mutations for 5-fluorouracil-(5FU)-resistance at this locus led us to clone and sequence the semi-dominant fur 1–5 allele. A single point mutation, resulting in the substitution of arginine 134 for serine, is responsible for this mutant phenotype. The fur 1–5 allele is transcribed and expressed at the same level as the wild-type allele. But, in contrast with the wild-type, the UPR Tase activity of the fur 1–5 mutant strain is stimulated in vitro by UTP and does not, therefore, correspond to a loss of feedback of UPR Tase activity. We found that uracil, as a free base, induces a significative increase in transcription and UPR Tase activity in a wild-type strain as well as in uracil-overproducing mutants which principally explains the high efficiency of the pyrimidine salvage pathway in S. cerevisiae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00309592
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  • 5
    Electronic Resource
    Electronic Resource
    Cloning and sequencing of URA10, a second gene encoding orotate phosphoribosyl transferase in Saccharomyces cerevisiae (1990)
    Springer
    Current genetics 17 (1990), S. 105-111 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Orotate phosphoribosyl transferase ; Nucleotide sequence-5-phosphoribosyl 1-pyrophosphate (5PRPP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Orotate phosphoribosyl transferase (OPRTase) catalyses the transformation of orotate to OMP in the pyrimidine pathway. In the yeast Saccharomyces cerevisiae, the URA5 gene is known to encode this enzyme activity. In this paper we present the cloning and sequencing of a yeast gene, named URA10, encoding a second OPRTase enzyme. Comparison of the predicted amino acid sequences between URA5 and URA10 genes shows more than 75% similarity. These sequences have also been compared to those of Escherichia coli, Podospora anserina, Sordaria macrospora and Dictyostelium discoideum. Remarkable similarities in the primary structure of these proteins have been found. Gene disruption experiments revealed that URA10 gene expression is responsible for the leaky phenotype of a ura5 mutant. Assays of OPRTase activity in extracts from ura5 and ura10 mutants indicate that the URA10 product contributes only 20% of the total activity found in wild type cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312853
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  • 6
    Electronic Resource
    Electronic Resource
    Regulation of gene expression and biological activity of rainbow trout estrogen receptor (1997)
    Springer
    Fish physiology and biochemistry 17 (1997), S. 123-133 
    Details
    ISSN: 1573-5168
    Keywords: reproduction ; steroid receptors ; estradiol ; estrogen receptor ; vitellogenin ; liver ; hepatocyte ; gene transcription ; mRNA stability ; fish ; rainbow trout
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rainbow trout estrogen receptor (rtER) concentration was highly induced in the liver after in vivo estradiol (E2) treatment or in vitro, in hepatocyte aggregate culture. Determination of transcription rate and mRNA half-life demonstrated that E2-induction of hepatic rtER level is caused essentially by an increase in the transcriptional and post-transcriptional activity of rtER gene. However, the expression of rtER gene in the liver seems to be down-regulated by glucocorticoids. We have used transient transfection assays with reporter plasmids linked to 5′ flanking regions of the rtER gene promoter, to identify cis-elements responsible for E2 inducibility. Deletion analysis localized a functional estrogen-responsive-element (ERE), near the transcription start site, with one mutation on the first base compared to the consensus sequence. This element and 200 bp fragment of the rtER promoter encompassing the ERE appear to be the major cis-acting element involved in the regulation of the gene. Data obtained from transfection experiments and footprinting analysis, suggested that the receptor is one of the major trans-factors implicated in the regulation of its own gene. However, interaction of ER with other transcription factors is required for maximal E2-stimulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007706207857
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