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  • 1
    Electronic Resource
    Electronic Resource
    The size of small polydisperse circular DNA (spcDNA) in angiofibroma-derived cell cultures from patients with tuberous sclerosis (TSC) differs from that in fibroblasts (1991)
    Springer
    Human genetics 〈Berlin〉 87 (1991), S. 6-10 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell cultures were derived from angiofibromas of three patients with tuberous sclerosis (TSC), from the unaffected skin of these patients, and from the skin of five healthy donors. The length distributions of the small polydisperse circular DNA (spcDNA) fraction of these cell cultures were then analyzed. Nearly half the spcDNA molecules from the angiofibroma cultures were longer than 0.4 μm, whereas only about 7% exceeded this threshold in the spcDNA preparations from the skin fibroblast cultures. The percentage of the larger size class of spcDNA showed an increase at higher numbers of in vitro passages in all three types of cultures, but this effect was much more conspicuous in the angiofibroma-derived cultures than in those from the skin fibroblasts. An age-dependent increase in the overall amount of spcDNA was only seen in the angiofibroma-derived cultures. Our earlier finding of elevated amounts of spcDNA in angiofibroma cultures was confirmed in cultures from an additional TSC patient.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01213083
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  • 2
    Electronic Resource
    Electronic Resource
    Viroid Structure (1981)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Biophysics and Biomolecular Structure 10 (1981), S. 115-132 
    Details
    ISSN: 0084-6589
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.bb.10.060181.000555
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  • 3
    Electronic Resource
    Electronic Resource
    Buchbesprechungen (1970)
    Springer
    Naturwissenschaften 57 (1970), S. 314-320 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00609398
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  • 4
    Electronic Resource
    Electronic Resource
    Elektronenmikroskopische Darstellung, Längenverteilung und Konfiguration einsträngiger Desoxyribonucleinsäure im Cytochrom-c-Film (1964)
    Springer
    Radiation and environmental biophysics 2 (1964), S. 73-78 
    Details
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Description / Table of Contents: Summary Single-stranded DNA is visualized by electron microscopy after the denaturation procedure by alkali (Freifelder andDavison) and by spreading to a mixed DNA-protein film. The configuration is measured by the mean squares of end-to-end distances as a function of contour lengths. Single-stranded DNA is more flexible than double-stranded DNA under the same conditions but like native DNA differs markedly from the random coil, probably because of electrostatic interactions along the polyelectrolyte molecule. The exponential distribution of the contour lengths is explained as a random distribution of breaks in single strands similar to these of native DNA.
    Notes: Zusammenfassung Durch Alkali nachFreifelder undDavison einsträngig gemachte DNA aus Forellengonaden wird mittels Spreitung einer DNA/Protein-Lösung elektronenmikroskopisch dargestellt. Ihre Konfiguration, gemessen durch das mittlere Quadrat der Abstände der Molekülenden als Funktion der Konturenlänge, ist flexibler als bei zweisträngiger DNA unter gleichen Bedingungen, weicht aber wie diese infolge ihres Polyelektrolytcharakters vom statistischen Knäuel merklich ab. Die exponentielle Verteilung der Konturenlängen wird als statistische Verteilung der Bruchstellen gedeutet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01225499
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  • 5
    Electronic Resource
    Electronic Resource
    Herpes simplex virus type 1 long-term persistence, latency, and reactivation in infected Burkitt lymphoma cells (1991)
    Springer
    Archives of virology 117 (1991), S. 251-267 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The two herpes simplex virus type 1 (HSV-1) strains F and AK which differ in virus-cell interaction and in DNA organization, were used to establish persistently productive infections in Burkitt lymphoma-derived cell lines BJAB and Raji. Four such lines could be maintained over a period of three years. Like the uninfected parental lines, the persistently infected cells display a cyclic pattern of cell proliferation. The expression of HSV-1-specific antigens proved to be variable. As a consequence, virus yields also vary within a subcultivation period. Pooled human HSV antisera, when continuously present, suppress virus production (inducible latency) and support cell proliferation to higher rates. By contrast, removal of the antiserum after a certain period of cultivation leads to virus reactivation with a delay of 8 to 20 days. After cultivation periods of more than 3 to 12 weeks, replacement of HSV antiserum does no longer result in virus reactivation and even inducers fail to reactivate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310769
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  • 6
    Electronic Resource
    Electronic Resource
    HSV type 1 genome variants from persistently productive infections in Raji and BJAB cell lines (1995)
    Springer
    Archives of virology 140 (1995), S. 1195-1213 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We studied possible genomic changes occurring in herpes simplex virus type 1 (HSV-1) during long-term cell culture which served as a model system for persistence and latency studies as introduced earlier. Sixteen HSV-1 reisolates were isolated from persistently productive HSV-1 (strains F and AK)-infected Burkitt lymphoma cell lines Raji and BJAB at four different times. They were roughly characterized in plaque morphology, plaque size, and infectivity. The viral reisolate DNAs revealed deletions and insertions of up to 1,150 base pairs in fragments BamHI-B,-E,-F,-J,-V,-X, and in the L-terminal and junction fragments S and K. Results were confirmed by additional restriction enzyme analyses and DNA sequencing of selected genomic regions between map units 0.642–0.650, 0.763–0.778 and 0.887–0.934. There was a progressive increase in genomic variability over a three-year period. However, changes in DNA fragment size occurred at different rates, with some reisolates showing stability over several months. The selective pressure for HSV-1 (F and AK) genomic changes was stronger in Raji than in BJAB cells, and stronger for F than for AK strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01322746
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  • 7
    Electronic Resource
    Electronic Resource
    Physical properties of the DNA of bacteriophage SP50 (1967)
    Springer
    Molecular genetics and genomics 100 (1967), S. 39-55 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The following properties of the DNA of B. subtilis phage SP50 were established: Molecular weight (in Daltons) 102×106 (sedimentation velocity) 97×106 (viscosity) 97×106 (contour lengths of electron micrographs) Base Composition (in % GC) 41.7 (chemical analysis) 44 (melting point) 44 (buoyant density) No unusual bases were observed. The complementary strands of the DNA can be separated. The phage DNA has genuine single strand breaks. The number and distribution of such breaks appears to be determined by the host on which phages were grown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425774
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