Springer Online Journal Archives 1860-2000
Abstract In order to evaluate the effects ofw-3 polyunsaturated fatty acid on BSA nephritis in female NZB/W F1 mice, animals were fed a diet containing fish oil (FO), safflower oil (SO) or beef tallow (BT) as a lipid source. After preimmunization 4 times with BSA, 50 mg/kg of BSA was given intraperitoneally every day for 4 weeks. Proteinuria was demonstrated in 50% of FO (n=18), in 33% of BT (n=21), but only in 5% of SO (n=19,p〈0.005 vs. FO,p〈0.05 vs. BT) animals. Anti-BSA antibodies did not differ among the three groups. Circulating BSA-anti BSA immune complexes (log2 of the ratio to pooled standard sera) were −1.3±0.3 in FO, −2.2±0.4 in SO and −2.7±0.5 in BT (p〈0.05 vs. FO) at sacrifice at 18 weeks of age. Prostaglandin E2 and thromboxane B2 production by mashed renal cortex was markedly suppressed in FO (p〈0.001, vs. SO and BT). By light microscopy, FO showed more glomerular hypercellularity and mesangial increase than SO (p〈0.001). By immunofluorescence and electron microscopy, heavier mesangial and loop or subepithelial deposits were seen in FO than in SO (p〈0.005). Fish oil may be involved in immune complex formation and its clearance by suppressed prostanoid production, resulting in enhanced BSA nephritis.
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