Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Growth inhibition of human keratinocytes by MC903 (calcipotriol) is linked to dephosphorylation of retinoblastoma gene product (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the European Academy of Dermatology and Venereology 5 (1995), S. 0 
    Details
    ISSN: 1468-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim This paper compares the effects of MC903 (calcipotriol) and 1,25-dihydroxyvitamin D3[1,25(OH)2D3; calcitriol] on differentiation and proliferation of normal human keratinocytes cultured in serum-free medium. In order to understand the inhibitory mechanism of MC903, we examined its effect on cell cycle kinetics and the phosphorylation of retinoblastoma gene product (pRB), a tumor suppressor gene products, in normal human keratinocytes.Background The hormonally active form of vitamin D3, 1,25-dihydroxy vitamin D3 [1,25(OH)2D3], regulates the differentiation and proliferation of epidermal keratinocytes in vitro. MC903 is a novel vitamin D3 analogue which is at least 100 times less potent than 1,25(OH)2D3 in its effects on calcium homeostasis.Methods We analyzed cell differentiation and cell cycle by flow cytometry using a FACScan, and pRB phosphorylation by Western blotting and densitometer.Results MC903 induced growth inhibition and differentiation of human keratinocytes. Cell cycle analysis demonstrated that 10-6 M of MC903 induced cell cycle arrest in both G1/G0 (62.4 ± 0.7% versus 56.5 ± 1.7% in control, p 〈 0.01) and G2 + M (19.2 ± 0.3% versus 14.0 ± 0.9% in control, p 〈 0.01) phase. 10-6 M of MC903 also increased the depnosphorylated pRB from 25% at 0 h to 84% at 48 h, as well as 1,25(OH)2D3.Conclusions Since pRB phosphorylation is supposed to be essential for the progression from G1 to S phase, the inhibition of pRB phosphorylation could be responsible for the G1/G0 growth arrest induced by MC903 in normal human keratinocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1468-3083.1995.tb00532.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Influence of a circadian-stage-dependent dosing schedule on the pharmacokinetics of isepamicin in humans (1996)
    Springer
    Journal of infection and chemotherapy 2 (1996), S. 106-109 
    Details
    ISSN: 1437-7780
    Keywords: Isepamicin ; chronopharmacokinetics ; human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract These experiments were conducted in order to determine the influence of the time of day of drug administration on the pharmacokinetics of isepamicin. Six healthy volunteers were given 400 mg isepamicin IM, on 2 separate occasions, either in the morning (8 AM) or in the evening (8 PM). Within-subject differences in the pharmacokinetic parameters between the morning and evening dosing regimens were evaluated. The plasma concentrations of isepamicin were not significantly different between the morning and evening trials, but significant time-dependent changes were found with a lower elimination rate constant and a longer elimination half-life in patients administered isepamicin at night. Our finding suggests that isepamicin may have the same clinical effects irrespective of whether dosing takes place in the morning or in the evening, but its clearance tends to be depressed when taken in the evening. Therefore, morning therapy is desirable because of possible interference from aminoglycoside toxicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02350851
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Characterization of Na ion-sensitive solvent polymeric membranes based on a neutral carrier (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 30 (1985), S. 487-496 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The states in the surface and the bulk phases of Na ion-sensitive polymeric membranes based on a synthetic carrier were investigated using SEM, IR, 13C-NMR, and GC. The 13C-NMR study revealed that conformation change of the carrier took place when the carrier was incorporated into the membrane phase. From SEM, IR, and 13C-NMR experiments with a deteriorated membrane, the conformation change of the carrier was proposed as one of the deterioration factors other than the decrease in the diffusion coefficient of the carrier in the membrane phase.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1985.070300204
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...