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  • 1
    Electronic Resource
    Electronic Resource
    Translational mobility of Concanavalin A receptors in normal and neoplastic glial cells (1982)
    Springer
    Acta neuropathologica 58 (1982), S. 215-223 
    Details
    ISSN: 1432-0533
    Keywords: Normal glia ; Neoplastic glia ; Concanavalin A receptors ; Colchicine ; Cytochalasin B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The dynamics of cell-associated Concanavalin A (Con A) in astrocytes of the newborn rat (RNA), the rat glioma (AC), and the human glioblastoma (GB) were studied in vitro by fluorescence and electron microscopy. Con A receptors on the cell surface were seen usually as a continuous thin layer, and Con A accumulations in fluorescence microscopy were actually Con A receptors on complicatedly infolded cell membrane and collection of Con A pinosomes. No capping occurred in the three types of glial cells. The translational movement of Con A receptors on the cell surface was rapid in the AC, slow in the RNA, and intermediate in the GB, and partly associated with Con A internalization. Con A pinosomes were more numerous in the RNA compared with those in the AC and the GB. Colchicine accelerated the translational mobility of surface Con A receptors more markedly in the AC and the GB than in the RNA. The translational movement Con A receptors, when treated with cytochalasin B, was retarded in the RNA and the GB and rather accelerated in the AC. Con A pinosomes were decreased in the three types of glial cells by treatment with colchicine or cytochalasin B.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690804
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  • 2
    Electronic Resource
    Electronic Resource
    Development of stroma in malignant lymphomas of the brain compared with epidural lymphomas (1986)
    Springer
    Acta neuropathologica 71 (1986), S. 125-129 
    Details
    ISSN: 1432-0533
    Keywords: Malignant lymphoma ; Fibronectin ; Factor VIII-related antigen ; Glial fibrillary acidic protein ; Amyloid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The relation of lymphoma cells to gliomesenchymal stroma within nervous tissue was studied by peroxidase-antiperoxidase immunostaining of formalin-fixed and paraffin-embedded surgical specimens for fibronectin (FN), factor VIII-related antigen and glial fibrillary acidic protein in 17 malignant non-Hodgkin lymphomas of the brain. For comparison, 9 non-Hodgkin lymphomas, 6 Hodgkin lymphomas, and 19 plasmacytomas of the spinal or cranial epidural spaces were studied with the same methods. Lymphoma cells were consistently negative for all markers. All lymphomas of the brain showed conspicuous concentric perivascular circles of immunoreactivity for FN in parts infiltrating brain tissue. Such structures are considered to derive from splitting of basal laminae of preexisting brain vessels; they were not seen in tumors of the epidural space. Cells with conspicuous FN content were found in brain as well as in epidural lymphomas. A monohistiocytic origin of those cells was confirmed by presence of monohistiocytic markers lysozyme and α-1-antichymotrypsin. Thus, additional immunostaining for FN seems to be useful for detecting monohistiocytes/macrophages in brain tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687973
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  • 3
    Electronic Resource
    Electronic Resource
    Lectin histochemistry of human gliomas (1989)
    Springer
    Acta neuropathologica 79 (1989), S. 176-182 
    Details
    ISSN: 1432-0533
    Keywords: Histochemistry ; Lectins ; Gliomas ; Macrophages
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Formalin-fixed and paraffin-embedded specimens of 24 human gliomas were examined histochemically with five lectins: concanavalin A (Con A), wheat germ agglutinin (WGA), Ricinus communis agglutinin 1 (RCA-1), peanut agglutinin (PNA), and Ulex europaeus agglutinin 1 (UEA-1). Although the staining intensity with lectins was variable, tumor cells in five astrocytomas, three oligodendrogliomas, six ependymomas, and one gliosarcoma, were generally positive for Con A, WGA, and RCA-1, and negative for PNA and UEA-1, whereas those in nine glioblastomas were usually positive for Con A and WGA and negative for RCA-1 and PNA as well as UEA-1. The malignancy in neoplastic astrocytes was correlated with the decrease in binding with lectins, especially RCA-1. Blood vessels, particularly the endothelial layers, in all gliomas were stained intensely with all lectins used. Macrophages showed two staining features with lectins; stippled and granular. The former macroplìages were positive for Con A, WGA, RCA-1, and PNA, and negative for UEA-1, whereas the latter macrophages were positive for all lectins used. Thus, the staining characteristics with lectins of macrophages were different from those of any glioma cells and very useful for identification of macrophages in gliomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294376
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  • 4
    Electronic Resource
    Electronic Resource
    Immunohistochemical study of fibronectin in human glioma and meningioma (1983)
    Springer
    Acta neuropathologica 59 (1983), S. 119-126 
    Details
    ISSN: 1432-0533
    Keywords: Fibronectin ; Glioma ; Meningioma ; Microscopic angiogenesis grading system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The presence of fibronectin (FN) and glial fibrillary acidic protein (GFAP) in two astrocytomas, 17 glioblastomas, and five meningiomas was studied by indirect immunoproxidase staining of formalin-fixed and paraffin-embedded surgical specimens. Angiogenesis in tumor was scored by the microscopic angiogenesis grading system (MAGS), and plasma FN levels were measured by single radial immunodiffusion. In astrocytomas and glioblastomas, GFAP-positive tumor cells had no FN expression and FN was confined to proliferating vessel walls and the leptomeninges, showing a mutually exclusive FN and GFAP expression. GFAP-positive tumor cells were occasionally surrounded by a network of FN-positive matrix produced by cells derived from the leptomeninges or blood vessels. In meningiomas, FN expression was found in vessel walls and meningioma cells including whorl formations and psammoma bodies. In general, deep immunoperoxidase staining for FN was shown in the endothelial cells and the psammoma bodies. Plasma FN levels were correlated significantly not to the degree of leptomeningeal proliferation but to the MAGS scores in gliomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691597
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  • 5
    Electronic Resource
    Electronic Resource
    Immunohistochemical study of fibronectin in hemangioblastomas and hemangiopericytomas (1984)
    Springer
    Acta neuropathologica 64 (1984), S. 229-233 
    Details
    ISSN: 1432-0533
    Keywords: Fibronectin ; Glial fibrillary acidic protein ; Hemangioblastoma ; Hemangiopericytoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Eight hemangioblastomas and two hemangiopericytomas were studied using indirect immunoperoxidase stains for fibronectin (FN) and glial fibrillary acidic protein (GFAP) in formalin-fixed, paraffinembedded surgical specimens. Stromal cells in hemangioblastomas were GFAP-negative and showed variable FN expression, while GFAP-positive cells were FN-negative, thus suggesting that the stromal cells are not derived from astrocytes. Hemangiopericytoma cells were poorly to intermediately FN-positive. The origin of stromal cells is discussed in the light of their fine structure and the immunohistochemical stains with other cell markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688113
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  • 6
    Electronic Resource
    Electronic Resource
    Interspecies comparison of c-myc gene in human and rat glioma cell lines (1991)
    Springer
    Acta neuropathologica 81 (1991), S. 382-387 
    Details
    ISSN: 1432-0533
    Keywords: Gliòma ; c-myc ; gene ; c-myc transcript ; c-myc protein ; In situ hybridization ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Interspecies difference in expression of the c-myc gene between two human and three rat glioma cell lines was studied with use of a human c-myc probe. The c-myc deoxyribonucleic acid (DNA) fragments detected at higher stringency in Southern blotting, showed a difference in size and gene copy number between human and rat glioma cells. The c-myc transcript was detected at both higher and lower stringencies in Northern blotting in human glioma cells, whereas it was demonstrated only at lower stringency in rat glioma cells, and the c-myc transcript was seen in cytoplasms of both glioma cells by in situ hybridization. The c-myc protein, if examined with anti-human c-myc protein monoclonal antibody, was observed as two separated components in Western blotting and localized immunocytochemically in nuclei in human glioma cells, whereas it was detected as three separate forms in Western blotting and shown in both nuclei and cytoplasm in rat glioma cells. The above discrepancy in manifestation of c-myc DNA fragments, transcript and protein could be due to the difference in nucleotide sequence of c-myc gene between human and rat glioma cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293458
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  • 7
    Electronic Resource
    Electronic Resource
    Contribution of histiocytic cells to sarcomatous development of the gliosarcoma (1987)
    Springer
    Acta neuropathologica 73 (1987), S. 124-130 
    Details
    ISSN: 1432-0533
    Keywords: Gliosarcoma ; Glioblastoma ; Fibronectin ; Histiocyte ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The expression of glial fibrillary acidic protein, fibronectin (FN), factor VIII-related antigen (FVIII/RAG), and of three monohistiocytic markers, lysozyme, α-1-antitrypsin and α-1-antichymotrypsin was examined in five gliosarcomas (GS) by peroxidase-antiperoxidase immunostaining of formalinfixed and paraffin-embedded specimens, and compared with vascular changes in 16 glioblastomas (GB). In contrast to GB, endothelial proliferations of GS were sheathed by sarcomatous tissue (perivascular sarcoma), which was contiguous with fibrosarcomatous areas. Cells with conspicuous intracytoplasmic FN content (FN+ cells) were seen in the vascular stroma of GB and dominated in the sarcomatous parts of GS. Most FN+ cells of GS were of varying size and shape and clearly neoplastic. Monohistiocytic markers were demonstrable in small infiltrating mononuclear cells as well as in many sarcomatous cells including FN+ cells. FVIII/RAG was restricted to lumen-lining endothelium and was not found in sarcomatous cells. These results suggest that a major part of sarcoma in GS is less likely to develop from proliferated endothelial cells than from histiocytic cells in the perivascular spaces of GB. By FN mediation, histiocytic cells might also guide and promote sarcomatous proliferations of other mesenchymal cells, leading to fibrosarcomatous development. Prominent monstrous giant cells of one GS seemed to be degenerating glioma cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00693777
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