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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 35 (1987), S. 758-760 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Analytica Chimica Acta 271 (1993), S. 31-38 
    ISSN: 0003-2670
    Keywords: Carbon-13 ; Collaborative studies ; Fruit juices ; Mass spectrometry ; Sugars ; Vegetable juices
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 11 (1984), S. 583-588 
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In recent years stable isotopes have begun to replace radioisotopes as tracers in metabolic studies. However, these are relatively expensive in the enrichments and quantities required by either conventional gas chromatography/mass spectrometry (GC/MS) or stable isotope ratio analysis (SIRA), the former having good sensitivity but low precision and the latter low sensitivity with more than adequate precision. We describe here a technique for 13C which achieves an appropriate balance between precision and sensitivity for such studies. A combustion interface is placed between the output from a capillary gas chromatograph and a dual collector SIRA mass spectrometer. Compounds eluting from the gas chromatograph are converted into discrete pulses of CO2 gas and intensities of 13CO2 and 12CO2 are measured simultaneously. Typically, 13C relative abundances can be obtained from 0.8 nmol of a C10 compound (8 nmol CO2) with a precision (1SD) of better than 1‰ (0.0011 at.%13C). This permits a reduction by at least a factor of 10 in the quantity of tracer required compared with conventional methods.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Gas chromatography/isotope ratio mass spectrometry (GC/IRMS) coupling was used to evaluate (13C)ethanol incorporation into plasmatic very-low-density lipoprotein (VLDL) triglycerides of three healthy human volunteers. After the perfusion of 13C-enriched alcohol, VLDL triglyceride fractions were extracted from plasma samples and prepared for the analysis of (13C)fatty acid methyl esters. The GC/IRMS coupling line, the analytical procedure and the data collection are described. The results show that ethanol itself may be used as a substrate for lipogenesis, though to a small extent: less than 10% of VLDL triglycerides may be derived from this metabolic pathway. Ethanol incorporates predominantly into myristic and palmitic acid. The small amount of sample material required for analysis, which minimizes the amount of isotope-labelled substrate required, makes this technique a valuable tool for metabolic investigations in human subjects.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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