ISSN:
1617-4623
Keywords:
Key words Resistance reaction
;
Powdery mildew
;
Yeast artificial chromosome (YAC)
;
Map-based cloning
;
Cleaved amplified polymorphic sequence (CAPS)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The barley Rar1 gene is an essential component of the race-specific, Mla-12-specified powdery mildew resistance reaction. As part of a map-based cloning strategy designed to isolate Rar1, five barley yeast artificial chromosomes (YACs) have been identified, ranging in size from 300 to 1100 kb. PCR-based YAC end-specific markers have been established and were employed to construct a local YAC contig. Four out of five YAC clones were found to be non-colinear with the source DNA. High-resolution genetic mapping of the YAC ends demonstrated that the set of five overlapping YAC clones encompasses the barley Rar1 gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380050874
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