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  • 1
    ISSN: 0378-1119
    Keywords: DNA and RNA hybridization ; Recombinant DNA ; contractile protein isoforms ; fetal ; nucleotide sequencing ; structure homology ; ventricle ; λgt11 cloning
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters A 194 (1994), S. 93-96 
    ISSN: 0375-9601
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. In leaves of three different cultivars of cowpeas (Vigna unguiculata), the fungal toxin fusi-coccin (FC) induced a plasmalemma depolarization from -175 to -100mV, a value slightly below the N2-determined diffusion potential in the dark, and to a lesser extent in the light. The depolarization was preceded by the usual initial membrane hyperpolarization (up to 18mV). The membrane depolarization was accompanied by considerable K+ efflux and extracellular alkalinization. Primary and secondary leaves as well as stem tissue of plants, grown under long-day conditions or in the dark responded similarly. Dark O2 uptake in leaves and hypocotyls was stimulated by FC by up to 77 and 87%, respectively. In contrast, FC caused a typical Em hyperpolarization, K+ influx, extracellular acidification and smaller stimulation of respiration (50%) in leaves of other legumes such as mungbean (Vigna radiata), or soybean (Glycine max). Leaves of navy beans (Phaseolus vulgaris) revealed an intermediate response to FC. The unusual effect of FC in Vigna might be related to the production of toxic catabolites during degradation and fermentation of storage products necessary to meet the strong energy requirement of the pm-H+ ATPase.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1435-1463
    Keywords: Keywords: Alzheimer's disease ; amyloid ; muscarinic receptors ; acetylcholine ; brain slices.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary. To examine the effects of the combined muscarinic m1-agonist/m2-antagonist Lu 25-109 on regulated processing of the amyloid protein precursor (APP), we used both transfected cells expressing human muscarinic m1 or m2 acetylcholine receptors, and fresh rat hippocampal slices. Lu 25-109 readily stimulated APPs secretion from HEK 293 cells overexpressing m1, but not m2, receptors, as well as from the hippocampal brain slices. Time-course analyses revealed a rapid (5–35 minutes), and a delayed (55–75 minutes) secretory response to Lu 25-109 with distinct concentration profiles suggesting two distinct cell biological mechanisms. Both responses appeared to reflect post-translational mechanisms because levels of APP message were unchanged after 60 minutes of stimulation with Lu 25-109. In comparison to carbachol, Lu 25-109 had a significantly lower intrinsic activity at muscarinic m1 receptors, compatible with a pharmacological profile as a partial agonist at recombinantly expressed m1 receptors. In as much as stimulation of APPs secretion is associated with reduced formation of Aβ peptides, Lu 25-109 may be useful to reduce Aβ generation, and thus, slow amyloid plaque formation. Moreover, Lu 25-109 may be useful in promoting the known neurotrophic and neuroprotective biological functions of secreted APPs.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Intensivmedizin und Notfallmedizin 35 (1998), S. 630-640 
    ISSN: 1435-1420
    Keywords: Key words Telemedicine ; transtelefonic ECG transmission ; emergency medicine ; arrhythmia ; acute coronary syndrome ; Schlüsselwörter Telemedizin ; telefonische EKG-Übertragung ; Notfallmedizin ; Arrhythmie ; Ischämie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In dieser Arbeit wird über Erfahrungen der telefonischen Mehrkanal-EKG-Übertragung in einem städtischen Krankenhaus berichtet. Patienten erhielten ein EKG-Ableite- und Übertragungsgerät, nachdem Ausgangsdaten im PC des Überwachungszentrum erfaßt waren. Die Übertragung aktueller EKG-Aufzeichnungen erweist sich als zuverlässig und akzeptabel nach einer Modifizierung der Ableitung. Mit hoher Sensitivität, Spezifität und Vorhersagegenauigkeit lassen sich Arrhythmen und Ischämien erfassen. Die Mehrzahl der betreuten Patienten übermittelten EKG-Aufzeichnungen in den ersten 6 Monaten. Im Überwachungszentrum war rund um die Uhr ein Arzt erreichbar, der unter Kenntnis von Vor-EKG, Anamnese und Befunden den anrufenden Patient beraten konnte. Zahlreiche Notarzteinsätze und Notfalleinweisungen konnten so vermieden werden. Akute Ischämien und Arrhythmien wurden häufiger beobachtet. Diesen Patienten wurde zur sofortigen Arztkonsultation oder stationären Aufnahme geraten. Weitere Möglichkeiten zur EKG-Überwachung sind derzeit in Erprobung: Übertragung aus abgelegenen Urlaubsorten, aus dem Flugzeug, aus dem Notarztwagen. Ziel ist dabei eine rasche Erkennung bedrohlicher Krankheitsbilder. In Zukunft ist auch eine Übertragung verschiedener physiologischer Parameter am Notfallort möglich, ergänzt durch anamnestische und klinische Daten aus einer elektronischen Patientenakte (Patienten Card). Die telefonische EKG- und Biosignalübertragung könnte eine wichtige Ergänzung der Notfallmedizin werden, sofern die Kostenträger den kostensparenden Wert dieser Methode erkennen.
    Notes: Summary In this paper experiences with transtelephonic ECG transmission are reported. After patients have been instructed, they received a simple ECG recorder and transmitted the 9 or 12 lead ECG to the Central Monitoring Unit situated at the intensive care unit (ICU) of the community hospital. This hospital-based transtelephonic ECG surveillance center is served by experienced physicians on a 24 h basis. According to previous studies, this system proved reliable with a high predictive value. This analysis is concerned with patients monitored about one year. Most of the ECG calls were done within the first sixth months. As patients could be immediately advised by a physician of the ICU after sending their ECG and reporting their symptoms, a lot of emergency treatments (emergency car equipped with an emergency physician) could be avoided. Acute ischemic events and arrhythmias could reliably detected, these patients had been referred to their general practitioner or, if necessary, recommended to see the emergency room at the hospital. To date, there are some more projects with telephonic ECG transmission, such as transmission from airplane or from remote tourist places or from the mobile emergency unit. New development will take place in the near future such as transmission of several more biological signals (ECG, oxygen saturation, blood pressure) in combination with the patients medical file via a small card. This then would reduce costs in emergency medicine, if health care providers will acknowledge and accept these procedures.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Der Chirurg 70 (1999), S. 1156-1162 
    ISSN: 1433-0385
    Keywords: Key words: Wound management ; War surgery ; Antibiotic prophylaxis ; Piperacillin. ; Schlüsselwörter: Wundmanagement ; Einsatzchirurgie ; Antibiotikaprophylaxe ; Piperacillin.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Internationale Einsätze von Hilfsorganisationen in Krisengebieten und Auslandseinsätze der Bundeswehr, aber auch nicht auszuschließende Katastrophen im Inland mit einem Massenanfall von Verwundeten stellen bezüglich der medizinischen Versorgung besondere Herausforderungen dar. Diese sind oftmals geprägt durch logistische Engpässe, verlängerte Transportzeiten sowie besondere Verletzungsmuster mit hohem Keimeintrag und konsekutiv gesteigertem Infektionsrisiko. Abweichend von den traumatologischen Therapierichtlinien im zivilen Bereich muß daher für die Chirurgie im Einsatz häufig ein aggressiveres Wundmanagement gefordert werden. Parallel dazu sollte die Indikation für eine möglichst sofortige hochdosierte intravenöse ungezielte Antibiotikaprophylaxe sehr großzügig gestellt werden. Mit Substanzen wie Piperacillin/Tazobactam oder Ceftriaxon stehen heute wirksame Medikamente für solch eine frühzeitige, ungezielte Prophylaxe, wie sie im Einsatz zu fordern ist, zur Verfügung. Diese Prophylaxe kann ihre Wirksamkeit nur in Kombination mit einer schnellstmöglichen chirurgischen Intervention entfalten. Bei fehlenden lokalen oder systemischen Infektionszeichen sollte die Prophylaxedauer bis zum Ende des operativen Eingriffs, in der Regel nicht länger als 24 Std, fortgeführt werden. Bei auftretenden Infektionszeichen greifen Antibiotikaprophylaxe und -therapie ineinander, letztere muß nach Antibiogramm ggf. adaptiert werden.
    Notes: Summary. Medical treatment of injured patients by international missions of non-governmental organisations in crisis areas and out-of-area operations by troops, and also national disasters require special trauma management. Deviations from peacetime surgical guidelines are obligatory because of long-distance medical evacuation, the possibility of gaps in supply and the typical pattern of war injuries. Massive contamination combined with the high risk of infection is one typical attribute of wounds inflicted during a war or a disaster. In contrast to peacetime surgical guidelines, aggressive wound débridement is often needed. Timely and prophylactically prescription of a broad-spectrum antibiotic with a long half-life has great importance in the treatment of these wounds. Suitable antibiotics for these indications are: piperacillin/tazobactam and ceftriazon. Nevertheless the use of antimicrobial agents will only be effective with early surgical débridement.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    World journal of urology 14 (1996), S. S55 
    ISSN: 1433-8726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ultrasound examination and biopsy of the nonaffected testis was performed in 78 men with a unilateral testicular cancer. Each testis was measured in three planes and the volume was calculated using the formula of an ellipsoid. The ultrasonic texture of each testis was given a score ranging from 1 to 5 as follows: 1, very regular; 2, slightly irregular; 3, irregular with small echogenic points; 4, very irregular or with coarse echogenic points; and 5, irregular with demarcated areas raising suspicion of tumor. Biopsies were examined for the presence of tubules with carcinoma in situ (CIS), germinative epithelium, Sertoli cell only, and obliterations; the thickness of tubular membranes and the amount of Leydig cells were registered. The mean ultrasonic testicular volume was 12.88 ml (range 3–24 ml), which was smaller than that previously reported for normal men and larger than that previously reported for infertile men. The ultrasonic testicular volume was inversely correlated to the score. Score 4 was given to 46% of the testes (median score, 4), and the score distribution was different from that reported in normal men (median, 2) and in infertile men (median, 3). In all, 9 testes contained CIS tubules, and 8 of these were given score 4; 1 testis with CIS in only 5% of the tubules was given score 3. The predictive value of score 4 for the testis to contain CIS was 22.2%, and the predictive value of a score different from 4 that the testis would not contain CIS was 97.6%. We conclude that a large percentage of contralateral testes in men with unilateral testicular cancer have an abnormal echotexture and that CIS is most likely found in testes given score 4 by ultrasound.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In clinical practice arises an increasing need for bone substitute materials. The maininorganic part of bone is the hydroxyapatite (HA). A new hydroxyapatite formula was created by a sol-gel-process at low temperature level [4]. The aim of this investigation was to test the biodegradation and the induction of bone formation by this new material and to compare these versus conventional fabricated HA and ß-TCP. 30 one-year-old Goettingen minipigs were divided into five groups. Critical size defect (〉5 m3) in the mandible was treated differently in all 5 groups:-group I- filling with pure HA, which was fabricated by sol-gel-technique, group II- control, only gelatinous material was given, group III- conventional ß-TCP [Cerasorb®], in group IV- conventional HA [Endobone®] and in group V [Targobone®], a non denatureted bovine collagen matrix was used. Macroscopical and microscopicalinvestigations of the former defects were made eight months postoperatively. The bone formation was superior in the sol-gel-HA-group (group I) in comparison with the control groups (group II) and the conventional fabricated ceramics groups (III and IV). In the sol-gel-HA group, the biodegradation of this new biomaterial was considered to very good with a resorption rate of more than 98%; eight months postoperatively. In this group complete bone formation was seen in former defects. In the control group, only an incomplete bone formation with 48.4% of the defect area was noted. This difference was significant (p〈0,001). A less bone formation was also observed in group III and IV with 57.6% and 56.9%. The bovine non-denaturated collagen matrix (group V) leads to only 20% of new formed bone.The new calcium phosphate formula made by a sol-gel method seems to be superior and suitable for filling bone defects
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 8 (1994), S. 748-752 
    ISSN: 1432-2218
    Keywords: Economic considerations ; High-tech medicine ; Cost/benefit ; Video laparoscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract There has been increasing concern in the medical, business, and insurance communities and government agencies about the rising cost of health care. Since 1980 the cost of medical care has increased from $280 billion dollars per year to $670 billion dollars in 1990, and was estimated at $900 billion in 1993. Several factors have contributed to this increase, including the high cost of hospitalization and new expensive technology, such as laparoscopic cholecystectomy. This present cost analysis was undertaken to determine the cost variables in laparoscopic cholecystectomy to see if changes in physician, nursing, and administration work activities could decrease the cost of hospitalization. Fifty-four patients who had an uncomplicated elective cholecystectomy during a five-month period in 1993 were assessed in terms of cost components of hospitalization, including nursing unit labor costs, surgery personnel labor costs, supply/instrument cost, and ancillary costs. Nine general surgeons participated in this study, four of whom accounted for 71% of the annual volume of cases. This analysis was performed with the help of Baxter Corporate Consulting, a Division of Baxter Healthcare Corporation. From this study, it was found that the average cost for uncomplicated laparoscopic cholecystectomy patients was $1589±$223. The operating room and supply/instrument component costs were the two largest expenses, accounting for 42% of the total cost. Reimbursement from various insurance agencies were also evaluated, and it was determined that a contract made with a local HMO caused the hospital to lose an average of $443.00 per patient. Instrument cost was evaluated, showing that the surgeons who used disposable instruments had an average instrument cost of $806±$63 per case, whereas those using reusable products had an average cost of $303 per case. (A reprocessing cost of $200 was also added when reusable instruments were used. This included the cost of cleaning, repairs, and labor costs for this equipment.) The cost of disposable and reusable products was evaluated in terms of the initial purchase price, replacement charges, equipment maintenance charges, and labor costs for cleaning, repackaging and inspection, sterilization, and quality control. A second factor identified was that patients directed to an inpatient nursing unit incurred an average direct nursing labor cost of $124, whereas the cost was only $54 when the patient went to an out-patient unit. A third observation demonstrated that the operating room time averaged 203 min±31 min, and the length of the operative procedure accounted for only 96 min±20 min of the total operating room time. From this data it appears that, in hospitals with more than 300 laparoscopic cholecystectomy cases per year, the costs of using reusable and disposable instruments is similar. Time/motion studies in the operating room have also been instituted, along with changes in postoperative physician orders, to decrease the cost of the hospitalization.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1173-1174 
    ISSN: 1420-9071
    Keywords: Individual specific pheromone trails ; Leptothorax ; nest emigration ; ant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Leptothorax affinis lays chemical trails during nest emigration. Workers which carried colony members during nest movement refused trails of nest mates and searched for their own trails. The origin of the individual specific trail substance could not be localized.
    Type of Medium: Electronic Resource
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