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  • 1
    Electronic Resource
    Electronic Resource
    Identification of constitutive and herbicide inducible cytochromes P-450 in Streptomyces griseolus (1988)
    Springer
    Archives of microbiology 149 (1988), S. 406-412 
    Details
    ISSN: 1432-072X
    Keywords: Cytochrome P-450 ; Induction ; Herbicide ; Detoxification ; Isozyme ; Streptomyces griseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The elevated soluble cytochrome P-450 content of Streptomyces griseolus cells found after growth in the presence of sulfonylurea herbicides has been shown to be the result of the appearance of one predominant cytochrome P-450 form. This cytochrome P-450 is the major soluble protein found to increase in amount following herbicide treatment, and functions as part of a sulfometuron methyl hydroxylase system. A second minor inducible cytochrome P-450 has been observed only in cells grown in the presence of chlorimuron ethyl, and a third cytochrome P-450 has been found to be present in all cells independent of the presence of sulfonylurea inducers. The three cytochrome P-450 isozymes are distinguishable primarily by their anion exchange properties; however, spectral properties, substrate inducibility, and enzymatic activity provide several further distinguishing features. The recognition of these inducible, xenobiotic metabolizing cytochromes P-450 in S. griseolus provides the only known description of monooxygenase proteins related to herbicide metabolism in bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425579
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and characterization of Streptomyces griseolus deletion mutants affected in cytochrome P-450-mediated herbicide metabolism (1991)
    Springer
    Molecular genetics and genomics 227 (1991), S. 238-244 
    Details
    ISSN: 1617-4623
    Keywords: Streptomyces ; Deletion mutants ; Genetic instability ; Cytochrome P-450 ; Herbicide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Metabolism of sulfonylurea herbicides by Streptomyces griseolus ATCC 11796 is carried out via two cytochromes P-450, P-450SU1 and P-450SU2. Mutants of S. griseolus, selected by their reduced ability to metabolize a fluorescent sulfonylurea, do not synthesize cytochrome P-450SU1 when grown in the presence of sulfonylureas. Genetic evidence indicated that this phenotype was the result of a deletion of 〉 15 kb of DNA, including the structural genes for cytochrome P-450SU1 and an associated ferredoxin Fd-1 (suaC and suaB, respectively). In the absence of this monooxygenase system, the mutants described here respond to the presence of sulfonylureas or phenobarbital in the growth medium with the expression of only the suhC,B gene products (cytochrome P-450SU2 and Fd-2), previously observed only as minor components in wild-type cells treated with sulfonylurea. These strains have enabled an analysis of sulfonylurea metabolism mediated by cytochrome P-450SU2 in the absence of P-450SU1, yielding an in vivo delineation of the roles of the two different cytochrome P-450 systems in herbicide metabolism by S. griseolus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259676
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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