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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular evolution 44 (1997), S. 207 -213 
    ISSN: 1432-1432
    Keywords: Key words: Bamboo mosaic virus — Satellite RNA — Satellite-encoded protein — Sequence variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Satellite RNA of bamboo mosaic potexvirus (satBaMV) is a linear RNA molecule which encodes a 20-kDa nonstructural protein. Sequences of seven different satBaMV isolates from bamboo hosts in three genera showed 0.7% to 7.5% base variation which spanned the whole RNA molecule. However, the putative 20-kDa open reading frame was all preserved in these isolates. The phylogenetic relationship based on the nucleotide sequence did not show particular grouping of satBaMV from the host in one genus; neither was the grouping of satBaMV evident by location of sampling. Putative secondary structures of the 3′ untranslated regions showed a basic pattern with conserved hexanucleotides (ACCUAA) and polyadenylation signal (AAUAAA) located in the loop regions. Although the satBaMV-encoded 20-kDa protein is a nonstructural protein, its predicted secondary structure contains eight-stranded β-sheets which may form ``jelly-roll'' structure similar to that found in capsid protein encoded by satellite virus of panicum mosaic virus.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 26 (1994), S. 775-778 
    ISSN: 1573-5028
    Keywords: ribosomal RNA gene ; RNA processing ; secondary structure ; molecular phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A highly conserved sequence was found in rRNA gene internal transcribed spacer 1 (ITS1) among flowering plant species. The sequence, GGCRY-(4 to 7 n)-GYGYCAAGGAA (where Y=C or T; R=G or A) is located in the central region of ITS1, and is present in published sequences from a wide range of flowering plants. The rest of ITS1 is highly variable in sequence. Therefore, the conserved motif within ITS1 may have a key function in the processing of rRNA gene transcripts. Furthermore, identification of such a conserved motif will help facilitate alignment of sequences for phylogenetic analysis.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1615-6110
    Keywords: Ascomycetes ; Clavicipitaceae ; Balansieae ; Epichloë typhina ; Acremonium spp ; Grass endophytes ; coevolution ; ribosomal RNA gene ; DNA sequence polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Acremonium sect.Albo-lanosa (Fungi Imperfecti) includes beneficial, endophytic mycosymbionts of various grasses of the subfamilyPooideae, and also the anamorph of the grass choke pathogen,Epichloë typhina (Clavicipitaceae, Ascomycotina). These fungi are seed-disseminated, thus stably maintained for many host generations. To investigate the possibility of long-term coevolution, isolates ofE. typhina and anamorphs were obtained from eight grass species, sequences of their rRNA gene internal transcribed spacers were aligned with those from otherClavicipitaceae, and cladograms were generated by maximum parsimony. The results indicated that the nonpathogenic endophytes have not necessarily coevolved with their host species and that they arose fromE. typhina on multiple occasions.
    Type of Medium: Electronic Resource
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