ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
To evaluate the effectiveness of the cell-material in situ on joint resurfacing, a wovenfabric polyglycolic acid (PGA) treated with fresh chondrocytes was used for repairing cartilagedefects. Full-thickness defects were created in the weight-bearing surfaces of the femoralintercondylar fossa in a rabbit model. The defect was filled with and without PGA under surgicalcondition. Before implantation, chondrocytes were co-cultured with PGA for one day. The animalswere sacrificed at eight weeks after implantation and evaluated grossly and histological score.Morphological examination showed that for PGA/chondrocytes group, the repaired tissue appearedsimilar in color and texture to the surrounding articular surface. While for the untreated control, nocartilage-like tissue was observed at all defects, but connective fibrous tissue. Histological analysisrevealed neochondrogenesis and clusters of cartilage matrix with specific safranin-O staining for thePGA/cell group. The Gross and histological evaluation indicated a significantly higher score forPGA/cell group than for PGA and control group. These results suggest that the woven fabric PGAmay facilitate the formation of cartilage tissues by providing a biodegradable and good-handlevehicle for the delivery to and retention of organized cell matrix constructs in vivo site. It mighttherefore enhance neochondrogenesis because of the superior biodegradable and biocompatible ofPGA scaffold sheet, while the more suitable biological environment might sustain cell growth andin situ cell function, suggesting a promising candidate for functional tissue engineering of clinicalenvironment
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1197.pdf
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