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  • 1
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Expression of MMP-2 is associated with progression and lymph node metastasis of gastric carcinoma Aims: One important step in tumour invasion is the penetration of the basement membrane. Matrix metalloproteinases (MMPs) play a key role in the migration of normal and malignant cells through the basement membrane. The aim of this study was to investigate correlations between matrix metalloproteinase 2 (MMP-2) immunoreactivity and currently used classification systems and possible relationships between lymph node metastasis and MMP-2 expression. Methods and results: This prospective study analysed specimens obtained from 114 gastric cancer patients (mean age 64 years; range 33–86 years) who underwent gastrectomy with extended lymphadenectomy. All specimens were categorized according to UICC classification, WHO classification, tumour differentiation, Laurén classification, Ming classification and Goseki classification. Formalin-fixed paraffin-embedded tumour specimens were stained using an avidin–biotin complex peroxidase assay. MMP-2 expression in the tumour epithelium was studied by immunohistochemistry with semiquantitative (score 0–3) evaluation. The MMP-2 staining pattern was positive (score 1–3) in 93 (81.6%) specimens and negative (score 0) in 21 (18.4%) samples. No significant correlations were found between MMP-2 expression and other variables such as age, tumour differentiation, WHO, Lauren, Goseki, and Ming classifications. In contrast, the intensity of MMP-2 staining in tumour cells correlated significantly with depth of tumour infiltration (T-stage), lymph node metastasis (N-stage), distant metastasis (M-stage), and UICC stage. Conclusions: Expression of MMP-2 is strongly associated with tumour progression and lymph node metastasis in gastric cancer. Therefore MMP-2 staining may be clinically useful as predictor of tumour progression, especially for lymph node metastasis.
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  • 2
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Comparative evaluation of the prognostic value of MUC1, MUC2, sialyl-Lewisa and sialyl-Lewisx antigens in colorectal adenocarcinoma Aims: The significance of MUC1, MUC2 and sialylated Lewis blood group antigens as prognostic markers in colorectal adenocarcinoma was investigated in a large series of patients because previous investigations revealed inconsistent results due to unrelated tumour samples from different patient groups and methodological differences. Methods and results: Tissues from 243 patients with colorectal adenocarcinoma were stained immunohistochemically. MUC1 showed a strong immunoreactivity (in more than 35% of the tumour area) in 32.5%, MUC2 in 51.0%, sialyl-Lewisx in 67.9% and sialyl-Lewisa in 73.7% of the cases, respectively. MUC1 immunoreactivity displayed a significant correlation with tumour progression as reflected by advancing pTNM staging and poor differentiation. MUC2 expression was significantly stronger in mucinous adenocarcinomas. Sialyl-Lewisx immunostaining correlated with the extent of lymph node metastasis as well as low cytological differentiation. According to univariate and multivariate analysis (P 〈 0.0001) only MUC1 reactivity represented a marker of worse survival probability, opposed to the sialylated Lewis antigens that did not exert a predictive value. Conclusions: According to our data, MUC1 and sialyl-Lewisx immunoreactivity exhibit statistically significant correlations with established markers of tumour progression. However, only MUC1 presents as an independent prognostic factor of colorectal adenocarcinoma.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aims:  Adenocarcinomas of the distal oesophagus and especially the oesophago–gastric junction have shown an increasing incidence during the last decade. Definition of subgroups according to different sites of development, histogenesis or aetiology may prove to be valuable for clinical diagnosis and treatment. Previous studies have shown differences in cytokeratin patterns between Barrett's metaplasia of the oesophagus and intestinal metaplasia in the stomach. The aim of our study was to investigate whether the expression of certain cytokeratins (CK7, CK20) and mucins (MUC1, MUC2, MUC5AC) exhibit clear-cut patterns, thus allowing a subclassification of adenocarcinomas of the oesophago–gastric junction. The possibility of a relationship between antigen expression and the presence or absence of Barrett's metaplastic epithelium was also studied.Methods and results:  CK7, CK20, MUC1, MUC2 and MUC5AC were visualized in six adenocarcinomas of the distal oesophagus, 29 adenocarcinomas of the oesophago–gastric junction and eight adenocarcinomas of the proximal stomach. CK7, CK20 and MUC1 were strongly expressed in the great majority of all neoplasms under study, whereas MUC2 and MUC5AC were absent or only faintly detectable. CK20 exhibited a significantly stronger expression in poorly differentiated tumours (G3) and MUC1 immunoreactivity correlated with tubular and papillary versus signet-ring cell histopathology. Other statistically significant correlations between antigens and histopathological features (pTNM stage, grading, histopathological subtype, presence/absence of Barrett's epithelium) were not observed.Conclusions:  According to our results, most adenocarcinomas of the oesophago–gastric junction show a CK7+, CK20+, MUC1+ phenotype irrespective of the presence or absence of Barrett's epithelium. The immunohistochemical data suggest a similar histogenesis of these tumours.
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  • 4
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study evaluates potential regulating factors in primary pulmonary carcinoid tumours, 16 typical and four atypical samples, with special emphasis on apoptosis and the bcl-2 gene family. Furthermore, p53-related oncogenes were analysed in a search for associated biological parameters.〈section xml:id="abs1-2"〉〈title type="main"〉Methods and resultsThe in-situ end-labelling technique (ISEL) was used to determine apoptotic cells, in addition to immunohistochemical methods, which were used to investigate the expression of the Ki67 antigen (avidin–biotin complex (ABC) method) and bcl-2, bcl-x, p53, p21/waf1, p27 and mdm-2 proteins (catalysed reporter deposition (CARD) technique). The incidence of apoptotic tumour cells was significantly enhanced in typical carcinoids. The bcl-2 protein was expressed to a higher degree in atypical carcinoids, which displayed a higher proliferative capacity as well. In contrast, bcl-x was observed predominantly in so-called typical carcinoids. The tumour cell turnover index was the most distinguishing parameter between both entities. All carcinoid tumours failed to show a staining for p53, p21/waf, p27 and mdm-2 proteins.〈section xml:id="abs1-3"〉〈title type="main"〉ConclusionsThe different biological behaviour of the carcinoid tumours under study seems to be influenced by the bcl-2 gene family preventing programmed cell death. We speculate that this results in a more aggressive course in atypical carcinoid tumours.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Der Chirurg 68 (1997), S. 196-198 
    ISSN: 1433-0385
    Keywords: Key words: Hans von Haberer ; Reconstructive vascular surgery ; Carotid surgery. ; Schlüsselwörter: Hans von Haberer ; rekonstruktive Gefäßchirurgie ; Carotischirurgie.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Hans von Haberer (1875–1958) konnte als Ordinarius für Chirurgie an den Chirurgischen Universitätskliniken Innsbruck, Graz, Düsseldorf und Köln umfangreiche Erfahrungen in der rekonstruktiven Chirurgie der Gefäßaneurysmen gewinnen. So operierte er zwischen 1914 und 1948 insgesamt 421 Gefäßaneurysmen, wobei vorwiegend die direkte, zirkuläre Gefäßnaht zum Einsatz kam. Bereits 1914 berichtete von Haberer von der ersten Rekonstruktion eines Carotisaneurysmas. Insgesamt operierte er 30 Carotisaneurysmen und ist somit der Pionier der rekonstruktiven Carotischirurgie. Anhand der ausführlichen Operations- und Krankenakten, die Hans von Haberer von 1904 bis 1949 bei annähernd 16 000 Eingriffen geführt hat, sowie der zeitgenössischen Literatur, werden die gefäßchirurgischen Erfahrungen von Haberers dargestellt.
    Notes: Summary. Hans von Haberer (1875–1958) gained wide experience in the reconstructive surgery of vascular aneurysms at the universities of Innsbruck, Graz, Düsseldorf and Cologne. In this period he operated on 421 vascular aneurysms – including 30 carotid aneurysms – mainly by means of direct circular vascular suture. In 1914 von Haberer described the first repair of a carotid aneurysm. Therefore he is the pioneer of reconstructive carotid surgery. Based on detailed clinical and operation reports on approximately 16 000 cases, written by Hans von Haberer between 1904 and 1949, and on the contemporary literature, we describe his experience in vascular surgery.
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  • 6
    ISSN: 1433-0385
    Keywords: Key words: Primary and secundary form of Baker's cyst ; Extirpation. ; Schlüsselwörter: Primäre und sekundäre Baker-Cyste ; Exstirpation.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. In der Unfallchirurgischen Universitätsklinik Köln wurden von 1988 bis 1997 19 Patienten an einer Baker-Cyste operiert. Die subjektiven und objektiven Ergebnisse wurden mit Hilfe eines Fragebogens sowie einer klinischen und sonographischen Nachuntersuchung erfaßt (Rücklauf: 95 %). Der präoperative Status wurde durch klinische Untersuchung, Sonographie und Anfertigung von Röntgenaufnahmen des Kniegelenks ermittelt. Wir differenzierten zwischen der angeborenen primären Cyste (39 %) und der sekundären Form, die immer mit einer Kniebinnenpathologie assoziiert ist (61 %). Bei allen sekundären Cysten wurde eine Arthroskopie durchgeführt. Als postoperative Komplikationen traten in einem Fall ein revisionspflichtiges Hämatom und in einem anderen Fall ein punktionspflichtiger Erguß auf. Das Operationsergebnis wurde von 61 % der Patienten mit „sehr gut“ und von 39 % mit „gut“ bewertet. Alle nachuntersuchten Kniegelenke waren passiv und aktiv frei beweglich. In einem Fall kam es zu einem Rezidiv. Nach unserer klinischen Erfahrung sollten primäre Baker-Cysten immer entfernt werden. Bei der sekundären Cyste muß neben der Exstirpation die Bedeutung der arthroskopischen Diagnostik und Therapie sowie der Sanierung der Grundkrankheit diskutiert werden.
    Notes: Summary. Baker's cysts were treated operatively in 19 patients in the Department of Traumatology of the University of Cologne from 1988 to 1997. The subjective and objective results were evaluated with a questionnaire and a clinical examination and sonography (follow-up: 95 %). All patients were examinated before surgery, sonography and X-ray of the knee were performed. We differentiated between the congenital primary cyst (39 %), and the secondary form, which was always associated with an intraarticular lesion (61 %). Arthroscopy was performed in all secondary forms of Baker's cyst. Postoperative complications were two reinterventions due to one hematoma and one effusion. Patient's evaluation of operation result was “excellent” in 61 % and “good” in 39 % of cases. All knee joints had a full range of motion. There was only one case of a recurrent cyst. The primary form of Baker's cyst has always to be extirpated, according to our clinical experience. The extirpation of the secondary Baker's cyst and the relevance of arthroscopy and treatment of the basic disease have to be discussed.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 382 (1997), S. 19-24 
    ISSN: 1435-2451
    Keywords: Key words Abdominal surgery ; Long-term results ; Handicap ; Disabled person's pass ; Schlüsselwörter Abdominalchirurgie ; Langzeit-ergebnisse ; Behinderung ; Behindertenausweis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die unterschiedliche Bewertung von Langzeitergebnissen nach abdominalchirurgischen Operationen war für uns Anlaß, den postoperativen Verlauf an 3 exemplarischen Operationsgruppen aus der Sicht des Patienten, des Operateurs und des Versorgungsamts zu untersuchen. Hierbei wurden 2 Gruppen mit benignen Erkrankungen einer Gruppe mit maligner Erkrankung gegenübergestellt. Die klinischen Daten wurden retrospektiv erhoben. Daten zur postoperativen Befindlichkeit und zum Grad der Behinderung konnten durch eine schriftliche Patientenbefragung im März 1995 gewonnen werden. Bei der Auswertung standen 59 Patienten mit Sigmaresektionen bei Divertikulitis (30 weiblich, 29 männlich, Alter im Median 61,5 Jahre) und 347 Patienten mit selektiv proximaler Vagotomie (SPV) bei Ulcera duodeni (72 weiblich, 275 männlich, Alter im Median 46 Jahre) 158 Patienten mit Magenresektion bei Karzinom (56 weiblich, 102 männlich, Alter im Median 61 Jahre) gegenüber. Die Gruppenauswahl erfolgte aufgrund des jeweils großen Krankenguts und bereits vorliegender postoperativer Verlaufsstudien. Das postoperative Intervall der Befragung betrug in der Divertikulitisgruppe im Median 5 Jahre (Spannbreite: 2–7 Jahre), in der SPV-Gruppe im Median 12 Jahre (Spannbreite: 1–24 Jahre) und in der Magenkarzinomgruppe im Median 7 Jahre (Spannbreite: 1–17 Jahre). Nach SPV besaßen 34,6% und nach Sigmaresektion 35,6% der Patienten einen Grad der Behinderung (GdB). Nach Magenresektion bei Karzinom hatten 77,2% der Patienten einen Behinderungsgrad. In Kombination mit zusätzlichen Erkrankungen waren die Patienten laut Versorgungsamt häufig schwerbehindert. Einen Schwerbehindertenausweis besaßen 27,1% der Patienten mit Sigmaresektion, 27,4% der Patienten mit SPV und 75,9% der Patienten mit Magenresektion bei Karzinom. Das postoperative Ergebnis fiel nach subjektiven Kriterien der Patienten und aus der Sicht des Operateurs in den Gruppen mit amtlich anerkannter Behinderung nicht wesentlich schlechter aus als bei Patienten ohne Behinderung. In vielen Fällen muß daher die definitive Graduierung unserer Patienten durch das Versorgungsamt in Frage gestellt werden.
    Notes: Abstract The patient, the surgeon and the public health officer (Versorgungsamt) assess the long-term results of abdominal operations differently. We tried to objectivize these different assessments. The clinical data were collected retrospectively. Data on postoperative subjective state and degree of handicap were obtained in a written patient survey (March 1995). Two groups with benign diseases and one group with malignant disease were examined: 59 patients who had undergone sigmoidectomy for diverticulitis (30 female and 29 male patients, median age: 61.5 years), 347 patients subjected to proximal gastric vagotomy for duodenal ulcers (72 female and 275 male patients, median age: 46 years) and 158 patients who had undergone gastrectomy for gastric carcinoma (56 patients female, 102 patients male, median age: 61 years). The public health officer, classed 35.6% of the patients who had undergone sigmoidectomy and 34.6% of the patients who underwent vagotomy as officially handicapped with a stated grade of disablement, 77.2% of the patients with gastrectomy were officially classed as handicapped. When other diseases were taken into account in addition, 27.1% of the patients who had undergone sigmoidectomy, 27.4% of those who had undergone vagotomy and 75.9% of the patients with gastrectomy had received passes officially identifying them as severely handicapped persons. In the vast majority of cases, the combination of several different ailments had resulted in recognition of a graded handicap and severely disabled person's pass, skeletal, cardiac and circulatory ailments being the most frequent. In spite of this, the evaluation of post-operative course by our patients, the surgeon and the public health officer (as reflected in the official state classification) in terms of the degree of handicap clearly differed.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 381 (1996), S. 82-87 
    ISSN: 1435-2451
    Keywords: Bone distraction ; Segmental transport ; Corticotomy ; Bone defect ; Open fracture ; Complications ; Limb lengthening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei Unterschenkeldefekten 〉4 cm ist eine Spongiosaplastik oder Rippenspananlage zur Überbrückung nicht mehr ausreichend, so daß wir in diesen Fällen seit 1988 eine Kallusdistraktion nach Ilisarov mit externer Fixierung durch Implantate der AO/ASIF durchführen. Bis zum 1. B. 1995 wurden 15 Patienten mit Unterschenkeldefektfrakturen mit der Kallusdistraktion behandelt. Die durchschnittliche Defektgröße betrug 7 cm, so daß über 1 m Röhrenknochen erzeugt werden konnte. Es handelte sich um 11 Männer und 4 Frauen im Durchschnittsalter von 21,3 Jahren. Bei den Defekten war 3mal eine Knochenresektion bei Tumoren erfolgt und 12mal eine zweit- oder drittgradig offene Fraktur vorausgegangen, davon 6mal mit begleitender Osteomyelitis. Im Mittel waren 5,3 Operationen zum Aufbau erforderlich. Die Komplikationsrate betrug 53% bei einer Behandlungszeit von durchschnittlich 1 Jahr. Bei allen abgeschlossenen Behandlungen waren die Endergebnisse exzellent und gut. Eine Amputation konnte in allen Fällen vermieden werden. Kontraindikation bei Anwendung dieses Verfahrens ist eine fehlende Mitarbeit des Patienten. Durch die Kallusdistraktion besteht eine realistische Chance des Extremitätenerhalts bei schwerem Weichteil- und Knochenschaden. Durch die komplette körperliche Reintegrationsmöglichkeit bei erhaltener Extremität kann eine hohe Motivation bei den Patienten erreicht werden.
    Notes: Abstract In open tibial fractures with defects over 4 cm, spongiosaplasty is considered to be insufficient. Since 1988 we have tried to apply Ilisarov's ideas of callus distraction in combination with modern external fixation devices and AO/ASIF implants. By August 1995, 15 patients with severe tibial fractures had been treated. The bone defect averaged 7 cm. Thus, more than 1 m of tubular bone was produced. Eleven male and 4 female patients, averaging 21.3 years in age, were given this treatment. The defect was caused by resection of a malignancy in 3 cases and a second- or third-degree open fracture in 12 cases, accompanied by osteomyelitis in 6 cases. Reconstruction required an average of 5.3 operations. The complication rate was 53%, and the median duration of treatment was about 1 year. The final results were excellent or good. Amputation could be avoided in all instances. This treatment is contra indicated if the patient exhibits a lack of compliance. There is a realistic chance of salvaging the limb in cases of severe soft tissue and bone defects. In terms of economical considerations, this treatment is cost effective. Physical integrity and mobility without aid is the important motivation for these patients.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 41 (1996), S. 1238-1240 
    ISSN: 1573-2568
    Keywords: heterotopic pancreas ; gastric tumor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Heterotopic pancreas is seen in one of every 500 laparotomies or 0.55–13% of autopsies (4–6). Despite modern diagnostic procedures (endoscopy, endoscopic ultrasound) it is difficult to diagnose preoperatively. A 51-year-old patient with a 30-year history of recurrent gastric ulcers was diagnosed with a gastric wall tumor. Endoscopic biopsy sample showed normal gastric mucosa. Endoscopic ultrasound and contrast radiography were not able to specify the gastric wall tumor. The local excision and histologic preparation of the tumor showed heterotopic pancreatic tissue within the gastric submucosa without any signs of malignancy. Three years postoperatively the patient is without any complaints. There have been no further signs of gastric ulcers.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    European journal of epidemiology 12 (1996), S. 657-658 
    ISSN: 1573-7284
    Keywords: Epidemiology ; Incidence ; Peptic ulcer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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