ISSN:
1432-1750
Keywords:
Alveolar macrophages
;
Air pollution
;
Nitrogen dioxide
;
Cytokines
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Activated alveolar macrophages (AMs) secrete interleukine (IL)1β, IL-6, IL-8, tumor necrosis factor-α (TNF-α), and transforming growth factor-β (TGF-β), whose inflammatory and fibroblast-activating characteristics may play a role in the maintenance of pulmonary inflammatory processes and subsequent fibrosis. Human AMs were transferred to a gas cylinder and exposed to NO2 in concentrations ranging from 0.1 to 0.5 ppm in synthetic air for 30 min at 37°C. AMs were fixed on a polycarbonate membrane and placed on culture medium. A culture was established, with the exposed AM (nonstimulated or stimulated with 1 μg/ml lipopolysaccharide [LPS]), and the remaining cells were used to determine the cytokines. IL-1β, IL-6, and IL-8 were quantified by commercial enzyme-linked immunosorbent assay kits (ELISA kits). TNF-α was determined with a “sandwich” ELISA, using the biotin-streptavidin system. NO2 exposure of nonstimulated AM did not result in changes in IL-1β, IL-6, TNF-α, and TGF-β release, compared to the situation with control experiments. Exposure for 30 min to NO2 induced a significant decrease of LPS-stimulated IL-1β, IL-6, IL-8, and TNF-α (p 〈 .05). The release of TGF-β was not significantly affected by NO2 exposure. Cytotoxicity of AM was checked by trypan blue exclusion, with values ranging from 1.3 to 3.0%. NO2 exposure of LPS-stimulated AM resulted in a functional impairment of AM after NO2 exposure regarding IL-1β, IL-6, IL-8, and TNF-α. Neither the spontaneous nor the stimulated release of TGF-β were influenced by NO2.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00167951
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