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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 166 (1996), S. 116-121 
    ISSN: 1432-072X
    Keywords: Key wordsStreptococcus equisimilis ; skc gene ; DNA ; bending ; Circular permutation analysis ; Computer ; modeling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA sequences upstream of the core promoter region of the streptokinase gene (skc) from Streptococcus equisimilis H46A increase skc transcription more than tenfold in vivo. This promoter upstream region contains a segment of intrinsically bent DNA, the precise location of which was determined experimentally by circular permutation analysis and theoretically by computer prediction. Electrophoretic analysis of circularly permuted upstream DNA fragments placed the bend center approximately at position –100 with respect to the major transcription initiation site of skc. This position was in excellent agreement with the center of maximum curvature predicted theoretically. Knowledge of the precise location of the bend center will be useful for future studies of the possible effect of DNA bending on skc transcription.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 53 (1966), S. 17-32 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary 1. The gut, fat body and the haemolymph of cockroaches contain a lysozyme. The lytic activity of the organs displays considerable differences not only in separate species but also in various animals of the same species. In particular, there is no positive correlation between the lysozyme content of guts and fat bodies within one species. 2. It is shown that the intracellular symbionts of cockroaches are sensitive to host-lysozyme. As soon as these organisms are liberated and contact organ-debris or haemolymph, they are lysed. Within the bacteriocytes of the fat body, however, symbionts are fully protected against the action of the host enzyme. 3. Lysozyme content of the hosts and lysozyme sensitivity of the symbionts lead to the conclusion that symbionts cultivated by other authors are dubious isolations. Inactivation or elimination of the host lysozyme is a prerequisite for further attempts to isolate the symbionts. Up to now the symbionts of blattides have not been successfully cultured.
    Notes: Zusammenfassung 1. Die Blattiden enthalten im Darm, Fettkörper und in der Hämolymphe ein Lysozym. Die auf Lochplatten mit Micrococcus lysodeikticus bestimmte lytische Aktivität der Organe (ausgedrückt in Wirkungseinheiten je Gramm Frischgewicht und bezogen auf kristallines Lysozym aus Hühnereiweiß) zeigt beim Vergleich der einzelnen Arten wie auch bei verschiedenen Individuen derselben Art erhebliche Unterschiede. Insbesondere läßt sich innerhalb einer Art keine positive Korrelation zwischen den Lysozymgehalten der Därme und Fettkörper erkennen. 2. Es wird demonstriert, daß die intracellulären Symbionten gegen das Wirtslysozym empfindlich sind. Sobald sie aus den Bacteriocyten befreit werden und dabei in Kontakt mit lysozymhaltigen Organresten oder Hämolymphe bleiben, fallen sie der Lysis anheim, die zum Teil ihren Weg über die Bildung sphärischer Körper nimmt. In den Bacteriocyten des Fettkörpers sind die Symbionten dagegen weitgehend vor der Wirkung des Wirtsenzyms geschützt. 3. Der Nachweis von Lysozym in den Blattiden und der Lysozymempfindlichkeit der Blattidensymbioten zwingen neben anderen Umständen dazu, alle positiven Kulturversuche als Fehlisolierungen zu bewerten. Die Blattidensymbionten müssen dem Kreis der bis jetzt nicht kultivierbaren Mikroorganismen zugerechnet werden. Vorbedingung für Kulturversuche ist die Entfernung oder Inaktivierung des Wirtslysozyms im Kulturansatz.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0147-619X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Gene 34 (1985), S. 357-362 
    ISSN: 0378-1119
    Keywords: Dideoxy sequencing ; homology ; promoter ; signal peptide ; staphylokinase ; transcription terminator
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 29 (1967), S. 400-405 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Bioelectrochemistry and Bioenergetics 7 (1980), S. 513-516 
    ISSN: 0302-4598
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Bioelectrochemistry and Bioenergetics 29 (1993), S. 373-374 
    ISSN: 0302-4598
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Electroanalytical Chemistry 116 (1980), S. 513-516 
    ISSN: 0022-0728
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 3 (1989), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The gene specifying the group A streptokinase (ska) gene was cloned from an M type 49 strain of Streptococcus pyogenes and shown to express in Escherichia coli. The nucleotide sequence of the DNA fragment carrying ska was determined and compared to that of the group C streptokinase gene (skc). There is 90% sequence identity between the two genes, with highly conserved transcription and translation control regions. The deduced amino acid sequence of the group A streptokinase (SKA) contains the same number of amino acids as that of group C streptokinase, with 85% sequence identity between the two proteins. Among 440 amino acid residues specified by the coding sequence, there are 62 non-identical residues with 45 conserved and 17 non-conserved residues. The non-identical residues are located in two major regions, spanning residues 174 to 244 and 270 to 290, with 40 and 10 amino acid changes, respectively. The sequence differences provide an explanation at the molecular level for the previous findings of immunological and chemical heterogeneity among streptokinases produced by pathogenic streptococci.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 29 (1970), S. 44-49 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The virulent, transducing, DNA-containing group A streptococcal bacteriophages A5 and A25 are 58 mμ in head size and have a flexible, noncontractile tail with a length of 180 mμ. The morphologic outline of the head is hexagonal. Treatment of the bacteriophages with potentially lethal ultraviolet radiation produced damages which were repairable by the Hcr+ streptococcal host strain K56, the host-cell reactivable sector being 0.9. These properties together with a demonstrable serological cross reaction show a close relationship between the two phage strains.
    Type of Medium: Electronic Resource
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